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An analysis of the response of the plastid division genes to the internal and external environments

质体分裂基因对内外环境的响应分析

基本信息

批准号：
18570037
负责人：
YOSHIOKA Yasushi
金额：
$ 2.63万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

It is essential for cell functionality that plastids are replicated and allocated to each daughter cell during cell division. However, plastid division seems to have little effect on plant development because most mutants that affect chloroplast numbers grow normally. The crumpled leaf (crl) of Arabidopsis thaliana is one of the exceptions that display severe abnormalities in plastid division and plant development. Our results indicated that ftsZ1-1, a plastid division mutation of A. thaliana enhanced all of the abnormality in crl. These results suggested that inhibition of plastid division is one of the causes of morphological abnormalities in crl Our results also indicated that CRL is not required for the formation of FtsZ ring because FtsZ1-1: GFP was localized at plastid equator in crl. We showed that crl mutant contains cells lacking detectable plastids in cotyledon of mature embryos. Such cells were probably generated by an uneven partition of plastids to each daughter cell, because of the presence of the dividing cells in which plastids were allocated to one of the daughter cells in crl embryos. The absence of cells lacking detectable plastids in the accumulation and replication of chloroplasts 6 (arc6), another plastid division mutant of A. thaliana having no significant defects in plant morphology, suggests that the generation of cells without detectable plastids is one of the causes of developmental abnormalities in crl.

质体在细胞分裂过程中复制并分配给每个子细胞对于细胞功能至关重要。然而，质体分裂似乎对植物发育影响不大，因为大多数影响叶绿体数量的突变体都正常生长。拟南芥的皱叶（crl）是质体分裂和植物发育中表现出严重异常的例外之一。我们的结果表明，拟南芥质体分裂突变ftsZ1-1增强了crl的所有异常。这些结果表明，质体分裂的抑制是 crl 中形态异常的原因之一。我们的结果还表明，CRL 不是 FtsZ 环形成所必需的，因为 FtsZ1-1: GFP 位于 crl 中的质体赤道处。我们发现 crl 突变体含有在成熟胚胎子叶中缺乏可检测质体的细胞。这种细胞可能是由质体不均匀地分配给每个子细胞而产生的，因为在 crl 胚胎中存在分裂细胞，其中质体被分配给其中一个子细胞。叶绿体 6 (arc6) 是拟南芥的另一种质体分裂突变体，在植物形态上没有明显缺陷，在积累和复制过程中不存在缺乏可检测质体的细胞，这表明缺乏可检测质体的细胞的产生是 crl 发育异常的原因之一。