An anaysis of the gene of Arabidopsis thaliana that is involved in the cell division, the cell differentiation, and the plastid division

拟南芥参与细胞分裂、细胞分化和质体分裂的基因分析

• 批准号: 14540591
• 负责人: YOSHIOKA Yasushi
• 金额: $ 2.3万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14540591/
• 关键词: Chloroplasts; Plastid division; Arabidopsis; Protein import; Cell division; Cell differentiation; Plastids; オルガネラ; タンパク質シャペロン

We identified a novel mutation of a nuclear-encoded gene, designated crumpled leaf (crl), of Arabidopsis thaliana that affects the morphogenesis of all plant organs and division of plastids. Histological analysis revealed that planes of cell division were distorted in shoot apical meristems, root tips, and embryos in plants that possess the crl mutation. Furthermore, we observed that differentiation patterns of cortex and endodermis cells in inflorescence stems, and root endodermis cells were disturbed in the cr1 mutant. These results suggest that morphological abnormalities observed in the crl mutant were due to aberrant cell division and differentiation. In addition, cells of the crl mutant contained a reduced number of enlarged plastids, indicating that the division of plastids was inhibited in the crl. The CRL gene encodes a novel protein with a molecular mass of 30 kDa that is localized in the plastid envelope. The CRL protein is conserved in various plant species including a fern, and in cyanobacteria, but not in other organisms. This data suggests that the CRL protein is required for plastid division, and also plays an important role in cell differentiation and the regulation of the cell division plane in plants.

我们发现了一个新的突变的核编码基因，命名为皱叶（crl），影响所有植物器官的形态建成和质体分裂的拟南芥。组织学分析显示，在具有crl突变的植物中，茎尖分生组织、根尖和胚的细胞分裂平面被扭曲。此外，我们观察到花序茎中皮层和内皮层细胞的分化模式以及根内皮层细胞在cr1突变体中的分化模式受到干扰。这些结果表明，在crl突变体中观察到的形态异常是由于异常的细胞分裂和分化。此外，crl突变体细胞中增大的质体数量减少，表明crl中质体分裂受到抑制。CRL基因编码一种新的蛋白质，分子量为30 kDa，定位于质体包膜中。CRL蛋白在包括蕨类植物在内的各种植物物种和蓝细菌中是保守的，但在其他生物体中不是。这些数据表明CRL蛋白是质体分裂所必需的，并且在植物细胞分化和细胞分裂平面的调节中也起重要作用。

项目成果

Tomoya Asano: "A mutation of the CRUMPLED LEAF gene that encodes a protein localized in the outer envelope membrane of plastids affects the pattern of cell division, cell differentiation, and plastid division in Arabidopsis"The Plant Journal. (印刷中). (2004

Tomoya Asano：“编码位于质体外膜的蛋白质的 CRUMPLED LEAF 基因的突变影响拟南芥中的细胞分裂、细胞分化和质体分裂的模式”（2004 年出版）。

Tomoya Asano: "A mutation of the CRUMPLED LEAF gene that encodes a protein localized in the outer envelope membrane of plastids affects the pattern of cell division, cell differentiation, and plastid division in Arabidopsis"The Plant Journal. 38. 448-459

Tomoya Asano：“CRUMPLED LEAF 基因的突变编码位于质体外膜的蛋白质，影响拟南芥细胞分裂、细胞分化和质体分裂的模式”《植物杂志》。

Takaaki Ishikawa: "The GLOBULAR ARREST1 gene, which is involved in the biosynthesis of folates, is essential for late embryogenesis in Arabidopsis thaliana"The Plant Journal. 33. 235-244 (2002)

Takaaki Ishikawa：“参与叶酸生物合成的 GLOBULAR ARREST1 基因对于拟南芥晚期胚胎发生至关重要”《植物杂志》。

Asano T, Yoshioka Y, Kurei S, Sakamoto W, Sodmergen, Machida Y.: "A mutation of the CRUMPLED LEAF gene that encodes a protein localized in the outer envelope membrane of plastids affects the pattern of cell division, cell differentiation, and plastid divi

Asano T、Yoshioka Y、Kurei S、Sakamoto W、Sodmergen、Machida Y.：“CRUMPLED LEAF 基因的突变编码位于质体外膜的蛋白质，影响细胞分裂、细胞分化和质体的模式

Ishikawa T, Machida C, Yoshioka Y, Machida Y.: "The GLOBULAR ARREST1 gene, which is involved in the biosynthesis of folates, is essential for late embryogenesis in Arabidopsis thaliana"The Plant Journal. vol.33. 235-244 (2002)

Ishikawa T、Machida C、Yoshioka Y、Machida Y.：“参与叶酸生物合成的 GLOBULAR ARREST1 基因对于拟南芥晚期胚胎发生至关重要”《植物杂志》。