Profile of the glycolipid carbohydrate chains in the different epithelial regions of digestive tract and their involvement in synbiosis and infection of bacteria

消化道不同上皮区域糖脂碳水化合物链的分布及其与细菌共生和感染的关系

• 批准号: 18570115
• 负责人: IWAMORI Masao
• 金额: $ 2.57万
• 依托单位: Kinki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18570115/
• 关键词: sphingoglycolipid; Gram-positive bacteria; fucosyltransferase; teichoic acid; TLC-immunostaining; Lactobacillus; knock out mice; autoantibody; パーオキシダーゼ標識抗体; 細胞壁; 抗乳酸菌抗体; TCL-免疫染色; 細菌叢

In murine digestive tract, glycolipids were differentially distributed in the different regions, i.e. the globo-series glycolipids including Forssman antigens in the mesenchymal regions and the ganglio-series glycolipids in the epithelial regions. Under germ-free condition, Gg_4Cer was the dominant glycolipid in the epithelia of duodenum, jejunum and ileum, but IV^2Fuα-Gg_4Cer became the major one after transfer of mice to the conventional breeding condition. However, the gastric and colonic epithelia contained GM1, fucosyl GMl, Gg_4cer and IV^2Fucα-Gg_4Cer, whose amounts were not affected by the breeding conditions. The gene responsible for fucosylation of Gg_4Cer in the digestive tract was characterized to be Fut-2 by targeted deletion of Fut-1 and Fut-2 genes. Also, although fucosylated glycolipids were completely eliminated from the stomach, caecum and colon of Fut-2-null mice, they were detected in the small intestine, indicating the alterative utilization of Fut-1 gene in the small intestine.Since Gg_4Cer is known to be the receptor of Lactobacillus, antibodies against L. johnsonii and L .intestinalis were generated to determine the binding ability of glycolipids and the number of bacteria in the different region of digestive tract. The antibodies reacted with teichoic acid in the cell wall of bacteria and with Gg_4Cer in the digestive tract, indicating the possible involvement of bacterial infection in the onset and progress of human disease with anti-Gg_4Cer antibodies, such as Beret disease, and that careful application of antibodies is required for the binding analysis of bacteria with Gg_4Cer.

在小鼠消化道中，糖脂在不同区域的分布存在差异，即间质区为含Forssman抗原的全球型系列糖脂，上皮区为神经节型系列糖脂。无菌条件下，小鼠十二指肠、空肠和回肠上皮中以Gg_4Cer为主，转入常规饲养条件后以IV^2Fuα-Gg_4Cer为主。而胃和结肠上皮细胞中含有GM1、focusyl GMl、Gg_4cer和IV^2Fucα-Gg_4Cer，其含量不受饲养条件的影响。通过靶向缺失Fut-1和Fut-2基因，确定了消化道中负责Gg_4Cer聚焦化的基因为Fut-2。此外，虽然fut -2缺失小鼠的胃、盲肠和结肠中完全消除了集中的糖脂，但在小肠中检测到它们，表明Fut-1基因在小肠中的替代利用。由于已知Gg_4Cer是乳杆菌的受体，因此产生了针对L. johnsonii和L. testinalis的抗体，以确定糖脂的结合能力和消化道不同区域的细菌数量。抗体可与细菌细胞壁的特壁酸和消化道的Gg_4Cer反应，提示抗Gg_4Cer抗体可能参与了人类疾病（如贝雷帽病）的发生和发展，需要谨慎应用抗体对细菌与Gg_4Cer的结合分析。

项目成果

Increase of ceramide monohexoside and dipalmitoyl glycerophospholipids in the brain of Zellweger syndrome

齐薇格综合征大脑中神经酰胺单己糖苷和二棕榈酰甘油磷脂的增加

• 发表时间: 2007
• 期刊: Neuroscience Letter 417
• 作者: Yabe R;Higo R;Sugita K;Iwamori M.;Rie Yabe;Hiroyuki Osawa;Masao Iwamori;Makiko Saito;Mikio Momoeda;Makiko Saito
• 通讯作者: Makiko Saito

Prolactin-dependent Expression of GD la Ganglioside, as a Component of Milk Fat Globule, in the Murine Mammary Glands.

GD la 神经节苷脂（作为乳脂肪球的成分）在小鼠乳腺中的催乳素依赖性表达。

• 发表时间: 2007
• 期刊: J. Biochem.(Tokyo) 142
• 作者: Momoeda M;Fukuta S;Iwamori Y;Taketani Y;Iwamori M.
• 通讯作者: Iwamori M.

Alterations in the molecular species ofpiasmamogen phospholipids and glycomipids dueto peroxisomal dysfunction in Chinese hamster ovary-mutant Z65 cells by FABMS method

FABMS法研究中国仓鼠卵巢突变Z65细胞过氧化物酶体功能障碍引起的磷脂和糖脂分子种类的变化

• 发表时间: 2007
• 期刊: Journal of Chromatography B 852
• 作者: Xu X;Monjusho H;Inagaki M;Hama Y;Yamaguchi K;Sakaguchi K;Iwamori M;Okino N;Ito M.;Masao Iwamori;Makiko Saito
• 通讯作者: Makiko Saito

子宮内膜癌由来細胞の分化関連脂質,トランスポーター,および抗癌剤感受性について

子宫内膜癌来源细胞的分化相关脂质、转运蛋白和抗癌药物敏感性

• 发表时间: 2007
• 作者: Iwamori M;Iwamori Y;Kiguchi K;Kubushiro K;Ishiwata I;岩森 正男
• 通讯作者: 岩森 正男

抗癌剤耐性ヒト卵巣癌細胞に特異的なスフィンゴ糖脂質の分子種変化。

抗癌药物耐药性人卵巢癌细胞特异性鞘糖脂的分子种类变化。

• 发表时间: 2007
• 作者: 岩森 正男;他.
• 通讯作者: 他.