A study on the mechanism of interaction of bone marrow-derived immature thymocytes with thymic dendritic cells
骨髓源性未成熟胸腺细胞与胸腺树突状细胞相互作用机制的研究
基本信息
- 批准号:03680140
- 负责人:
- 金额:$ 1.09万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (C)
- 财政年份:1991
- 资助国家:日本
- 起止时间:1991 至 1992
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
A characteristic disappearance of thymocytes from the cortex of thymus was observed 48 hrs after intraperitoneal injection of cortisone acetate and the same alteration was also observed by radiation with 500rad of X-ray. However, immnosuppressive agents, cyclosporin A and FK 506 did not cause the reduction of the cortical thymocytes, with a slight loss of medullary thymocytes by FK 506. Reduction of the cortical thymocytes resulted in a reduced concentration of IV^3NeuGc-nLc_4Cer and VI^3NeuGc-nLc_6Cer, both of which were the marker gangliosides of cortisone-sensitive cortical thymocytes. At the same time, a significant increase in II^3NeuAc-LacCer, in both total amount and concentartion, was observed in both rabbit and mouse thymuses after cortisone administration. The specific activity of sialyltransferase, with LacCer and CMP-^<14>C-NeuAc as substrates, was the same in the thymus homogenates of cortisone-administered and control mice. Moreover, no difference was observed in the capacity to synthesize II^3NeuAc-LacCer from ^<14>C-ManNAc in the loose thymuses of cortisone-administered and control mice. However, sialyltransferase involved in the synthesis of II^3NeuAc-LacCer with CMP-^<14>C-NeuAc was only detected in the loose thymuses after cortisone-administration, that is, not in the controls, indicating that LacCer on the cell surface is characteristically sialylated by the plasma membrane-associated sialyltransferase only after cortisone administration. Since the cortisone-induced synthesis of II^3NeuAc-LacCer and activation of the sialyltransferase seemed to occur in thymus matrix cells, these observations might be related to the mechanism underlying detachment of cortisone-sensitive lymphocytes from the thymus matrix cells on modification of the lymphocyte receptor molecule.The analogical hormone-dependent regulation of a negative charge was observed in rabbit uterine endometrium after steroid administration.
腹腔注射醋酸可的松48小时后,胸腺皮质出现特征性的胸腺细胞消失,500 rad X线照射也出现同样的变化。然而,免疫抑制剂,环孢素A和FK 506没有引起皮质胸腺细胞的减少,与FK 506的髓质胸腺细胞的轻微损失。皮质胸腺细胞的减少导致皮质皮质胸腺细胞的标记神经节苷脂IV^3 NeuGc-nLc_4Cer和VI^3 NeuGc-nLc_6Cer浓度降低。与此同时,在给予可的松后,在兔和小鼠胸腺中均观察到II^3 NeuAc-LacCer的总量和浓度显著增加。以LacCer和CMP-1-C-NeuAc为底物的唾液酸转移酶的比活性<14>在可的松给药小鼠和对照小鼠的胸腺匀浆中相同。此外,在给予可的松的小鼠和对照小鼠的疏松胸腺中,从^C-ManNAc合成II^3 NeuAc-LacCer的能力没有观察到差异<14>。然而,参与II^3 NeuAc-LacCer与CMP-^C-NeuAc合成的唾液酸转移酶<14>仅在可的松给药后的疏松胸腺中检测到,也就是说,在对照中未检测到,这表明细胞表面上的LacCer仅在可的松给药后才被质膜相关的唾液酸转移酶特征性地唾液酸化。由于可的松诱导的II^3 NeuAc-LacCer合成和唾液酸转移酶的激活似乎发生在胸腺基质细胞中,这些观察结果可能与可的松敏感的淋巴细胞从胸腺基质细胞上脱落的机制有关,在类固醇给药后的兔子宫内膜中观察到类似的负电荷依赖性调节。
项目成果
期刊论文数量(24)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Kubushiro K, Tsukazaki K, Tanaka J, Kiguchi K, Iwamori M: "Human uterine endometrial adenocarcinoma-characteristic acquirement of synthetic potential for II3S03-LacCer and ganglio-series sulfoglycosphingolipids." Cancer Research. 52. 803-809 (1992)
Kubushiro K、Tsukazaki K、Tanaka J、Kiguchi K、Iwamori M:“人类子宫内膜腺癌 - II3S03-LacCer 和神经节系列磺基鞘糖脂合成潜力的特征性获得。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
MIKAMI M,NOZAWA S,IWAMORI M,NAGAI Y: "Menstrual cycle-associated expression of 2-hydroxy fatty acyl phytosphingosine containing GlcCer,LacCer and Gb3Cer in buman uterine endometrium" Biochimica Biophysica et Acta. 1125. 104-109 (1992)
MIKAMI M、NOZAWA S、IWAMORI M、NAGAI Y:“在布曼子宫内膜中含有 GlcCer、LacCer 和 Gb3Cer 的 2-羟基脂肪酰基植物鞘氨醇的月经周期相关表达”Biochimica Biophysica et Acta。
- DOI:
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- 影响因子:0
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- 通讯作者:
Kusunoki S,Inoue K,Iwamori M,Nagai Y,Mannen T,Kanazawa I: "Developmental changes of fucosylated glycoconjugates in rabbit dorsal root ganglia" Neuroscience Research. 15. 74-80 (1992)
Kusunoki S,Inoue K,Iwamori M,Nagai Y,Mannen T,Kanazawa I:“兔背根神经节中岩藻糖基化糖缀合物的发育变化”神经科学研究。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
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KIGUCHI K,NOZAWA S,IWAMORI M,NAGAI Y: "Glycosphingolipids of human various ovarian tumors:A significantly high expression of I^3SO_3-GalCer and Lewis antigen in musinous cystadenocarcinoma" Cancer Research. 52. 416-421 (1992)
KIGUCHI K、NOZAWA S、IWAMORI M、NAGAI Y:“人类各种卵巢肿瘤的糖鞘脂:I^3SO_3-GalCer 和 Lewis 抗原在鼠性囊腺癌中显着高表达”癌症研究。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Horikawa,K, Nakakuma H, Nagakura S, Iwamori M, Takatsuki K:"Hemolysis of human erythrocytes is a new bioactivity of gangliosides." Journal of Experimental Medicine. 174. 1385-1391 (1992)
Horikawa,K, Nakakuma H, Nagakura S, Iwamori M, Takatsuki K:“人红细胞溶血是神经节苷脂的一种新生物活性。”
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- 影响因子:0
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IWAMORI Masao其他文献
IWAMORI Masao的其他文献
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{{ truncateString('IWAMORI Masao', 18)}}的其他基金
Role of the glycolipid receptor in the digestive tract and bacterial glycolipid antigen in bacterial infection and symbiosis
消化道糖脂受体和细菌糖脂抗原在细菌感染和共生中的作用
- 批准号:
24570141 - 财政年份:2012
- 资助金额:
$ 1.09万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Profile of the glycolipid carbohydrate chains in the different epithelial regions of digestive tract and their involvement in synbiosis and infection of bacteria
消化道不同上皮区域糖脂碳水化合物链的分布及其与细菌共生和感染的关系
- 批准号:
18570115 - 财政年份:2006
- 资助金额:
$ 1.09万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Regulation and roles of the expression of milk fat globule-characteristic gangliosides in murine mammary gland.
小鼠乳腺乳脂肪球特征神经节苷脂表达的调控和作用。
- 批准号:
08680646 - 财政年份:1996
- 资助金额:
$ 1.09万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Concurrent regulation ofcholesterol sulfotransferase and sulfatase
胆固醇磺基转移酶和硫酸酯酶的同时调节
- 批准号:
05680519 - 财政年份:1993
- 资助金额:
$ 1.09万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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