权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Concurrent regulation ofcholesterol sulfotransferase and sulfatase

胆固醇磺基转移酶和硫酸酯酶的同时调节

基本信息

批准号：
05680519
负责人：
IWAMORI Masao
金额：
$ 1.28万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

项目摘要

Cholesterol sulfatein the endometrium of rabbit uterus was increasedin the concentration per gram dry weight by pregnancy, and the concentration in the pregnant endometrium was 140-fold than that in the nonpregnant endometrium. In the endometrium of pregnant rabbit, one tenth of the total cholesterol was sulfated at the maxmum level and both of cholesterol sulfotransferase and cholesterol sulfate sulfatase were simultaneously regulated to elevate the concentration of cholesterol sulfate. Synthesis of cholesterol sulfate was found to occure in the epithelial cells of uterine endometrium, but not in the stromal cells. Concerning on the mechanism of induction of cholesterol sulfate in the pregnant endometrium, the cell density was found to be important. A stimulation with progesterone to the sparce culture of the endometrial cells was unable to induce cholesterol sulfate, but activation of cholesterol sulfotransferase occurred to the confluent culture with progesterone, indicating that responsiveness of the endometrial cells to steroid hormones requires for the cells to differentiate in the sufficient stage. This findings coincided that the sulfotransferase appeared in the endometrium of rabbits 4 days after administration of gonadtropin to secrete progesterone, though the endometrium initiated the proliferation. the same activation was observed in The confluent stage of keratinocytes. On the other hand, cholesterol sulfate was found tobe selectively distributed in the epithelial cells in the digestive organs and kidney. Although the spspecific activities of cholesterol sulfotransferase and cholesterol sulfate sulfatase and the concentration of metabolic precurson cholesterol were significantly high in liver, a programd expression of cholesterol sulfate and transglutaminase was observed during the development of skin. In fact, cholesterol sulfate characteristically activated protein kinase n and was related to the induction of transglutaminase.

妊娠使兔子宫内膜中胆固醇硫酸酯每克干重浓度升高，妊娠子宫内膜中胆固醇硫酸酯浓度是未妊娠子宫内膜浓度的140倍。在孕兔子宫内膜中，将总胆固醇的1 / 10磺化至最高水平，同时调节胆固醇硫代转移酶和胆固醇硫酸盐硫酸酯酶，使硫酸胆固醇浓度升高。硫酸胆固醇的合成在子宫内膜上皮细胞中发生，而在基质细胞中不发生。关于妊娠子宫内膜诱导硫酸胆固醇的机制，发现细胞密度是重要的。孕酮刺激子宫内膜细胞空培养不能诱导硫酸胆固醇，但在孕酮融合培养中出现了胆固醇磺酸转移酶的激活，这表明子宫内膜细胞对类固醇激素的反应需要细胞在足够的阶段分化。这一发现与使用促性腺激素分泌黄体酮4天后，子宫内膜出现硫转移酶相吻合，尽管子宫内膜开始增殖。在角化细胞融合期观察到同样的激活。另一方面，硫酸胆固醇在消化器官和肾脏的上皮细胞中选择性分布。虽然肝脏中胆固醇硫转移酶和胆固醇硫酸酯酶的特异性活性以及代谢前期胆固醇的浓度显著升高，但在皮肤发育过程中观察到硫酸胆固醇和转谷氨酰胺酶的程序性表达。事实上，硫酸胆固醇特征性地激活了蛋白激酶n，并与谷氨酰胺转酶的诱导有关。