Function of LIM homeodomain protein Lhx2 in odorant receptor gene regulation

LIM同源域蛋白Lhx2在气味受体基因调控中的功能

• 批准号: 18570206
• 负责人: HIROTA Junji
• 金额: $ 2.5万
• 依托单位: Osaka Prefecture University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18570206/
• 关键词: olfactory; odorant receptor; gene expression; transgenic; transcription factor; Lhx2; 神経分化

Odorant receptor (OR) genes can be classified into two types: fish-like class I OR genes and terrestrial-specific class II OR genes. We have previously shown that Lhx2, a LIM-homeodomain protein, binds to the homeodomain site in the promoter region of mouse M71, a class II OR, and that a knockout mutation in Lhx2 precludes expression of all tested class II OR genes including M71. Here, we report that most class I OR genes, which are expressed in a dorsal region of the olfactory epithelium, are still expressed in Lhx2-deficient embryos. There are two exceptions: two class I OR genes, which are normally expressed in a more ventral region, are no longer expressed in Lhx2 mutant mice. Lhx2 is transcribed in olfactory sensory neurons irrespective of expression of class I or class II OR genes. Thus, a deficiency of Lhx2has a differential impact on class I and class II OR gene expression, suggesting that two distinct pathways underlie OR gene expression in mouse.

气味受体（OR）基因可分为两类：鱼类类I类OR基因和陆地特异性II类OR基因。我们之前已经证明，Lhx2，一种lim同源结构域蛋白，结合到小鼠II类OR M71的启动子区域的同源结构域位点，并且Lhx2的敲除突变阻止了包括M71在内的所有II类OR基因的表达。在这里，我们报告了在嗅觉上皮背侧表达的大多数I类OR基因在lhx2缺陷胚胎中仍然表达。有两个例外：两个通常在更腹侧区域表达的I类OR基因在Lhx2突变小鼠中不再表达。Lhx2在嗅觉感觉神经元中转录，与I类或II类or基因的表达无关。因此，缺乏lhx2对I类和II类OR基因表达有不同的影响，表明小鼠OR基因表达有两种不同的途径。

项目成果

Differential impact of Lhx2 deficiency on expression of class I and class II odorant receptor genes in mouse

• DOI: 10.1016/j.mcn.2007.01.014
• 发表时间: 2007-04-01
• 期刊: MOLECULAR AND CELLULAR NEUROSCIENCE
• 影响因子: 3.5
• 作者: Hirota, Junji;Omura, Masayo;Mombaerts, Peter
• 通讯作者: Mombaerts, Peter

Class dependent expression of odorant receptor genes in Lhx2 deficient mice

Lhx2 缺陷小鼠中气味受体基因的类别依赖性表达

• 发表时间: 2007
• 作者: Junji;Hirota;Masayo;Omura;Peter;Mombaerts
• 通讯作者: Mombaerts

Differential impact of Lhx2 deficiency on expression of class I and class II odorant receptor genes.〜Evolution and regulation of odorant receptor genes〜

Lhx2缺陷对I类和II类气味受体基因表达的差异影响〜气味受体基因的进化和调控〜

• 发表时间: 2007
• 作者: Junji;Hirota;Junji Hirota
• 通讯作者: Junji Hirota

Differential impact of Lhx2 deficiency on expression of class I and class II odorant receptor genes

Lhx2 缺陷对 I 类和 II 类气味受体基因表达的差异影响

• 发表时间: 2007
• 作者: Junji;Hirota;Junji Hirota;廣田 順二
• 通讯作者: 廣田 順二

鋤鼻神経系におけるBcl11bの機能解析

Bcl11b在犁鼻神经系统中的功能分析

• 发表时间: 2007
• 作者: Junji;Hirota;Masayo;Omura;Peter;Mombaerts;廣田 順二;廣田 順二
• 通讯作者: 廣田 順二