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Characterization of molecular mechanisms of sporophytic self-incompatibility in Ipomoea trifida

三裂蕹孢子体自交不亲和的分子机制表征

基本信息

批准号：
18580004
负责人：
TSUCHIYA Tohru
金额：
$ 2.49万
依托单位：
Mie University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18580004/
关键词：
Self-incomoatibility Wild type Sweet potato Ioomoea trifida Ipomoea trrfrda

项目摘要

To identify genes correlated to self-incompatibility(S-genes)of wild type sweet potato(Ipomoea trifida), genomic sequences of S-locus from S1, 53, S10, S29 self-incompatible lines and Sc, the self-compatible line, were isolated and sequenced.By the expression analysis and sequence polymorphism, several S-gene candidates were identified ; AB1 to AB4 as male S-gene candidates and SE1 to SE2 and SEA as female S-gene candidates. Among these genes, AB2, A83, SE2 and SEA were found from all analyzed S-locus sequence. In the Sc S-haplotype, however, some mutations were observed in the promoter sequence and gene itself in some of the S-gene candidates. These mutations may contribute the Sc-haplotype as self-compatible.To determine spatial and temporal expression of these four S-gene candidates, in situ hybridization analyses were carried out. In situ analyses indicated that all of the male part S-candidate genes were expressed in the tapetum at early developmental stage, and all of the female … More part S-candidate genes were expressed in the papilla cells of stigma at mature stage. These results indicated that all S-candidate genes share a necessary and sufficient condition for S-genes.Comparison between the genomic sequences of five S-haplotypes indicated that the existence of a regions which have highly sequence polymorphisms, designated as S-locus diversed regions(SDRs). Within this SDRs, three S-candidate genes were located, which are AB2, AB3 and SEA, therefore, these gene products might play key roles in the self-incompatibility reaction.Furthermore, comparison of the length of the SDRs from self-incompatible lines indicated that SDR length is longest in the most dominant S-haplotype and is shortest in the most recessive line, and the order of the SDR length is consistent with the dominance-recessive hierarchy of the self-incompatibility. In the dominance line have additional sequence to recessive line, such as transposon-like sequences, repetitive sequences, and fragments of organelle genome-like sequences. From this observation, it was considered that the lines at the higher hierarchy in the self-incompatibility of Ipomoea trifida was developed from recessive line. Less

为了寻找甘薯自交不亲和相关基因(S基因)，对甘薯自交不亲和系S1、53、S10、S29和自交不亲和系SC的S基因座的基因组序列进行了分离和测序，通过表达分析和序列多态性分析，确定了几个S基因的候选基因，其中ab1到ab4为雄性S基因候选，se1到se2和SEA为雌性S基因候选。在这些基因中，AB2、A83、SE2和SEA是从所有分析的S基因座序列中发现的。然而，在SC S单倍型中，观察到一些S候选基因的启动子序列和基因本身发生了一些突变。这些突变可能导致了SC单倍型的自交亲和。为了确定这四个S候选基因的时空表达，进行了原位杂交分析。原位分析表明，所有雄性部分的S候选基因在早期发育阶段都在绒毡层中表达，雌性…都在绒毡层中表达成熟时，S候选基因在柱头乳突细胞中表达较多。这些结果表明，所有S候选基因都具有S基因的充要条件。5种S单倍型的基因组序列比较表明，存在一个高度序列多态的区域，称为S基因座多态区域(SDR)。在该序列中定位了3个S候选基因ab2、ab3和SEA，因此这些基因产物可能在自交不亲和反应中起关键作用。此外，通过比较自交不亲和系的SDR长度，发现最显性的S单倍型的SDR长度最长，而最隐性的S单倍型的SDR长度最短，其大小顺序与自交不亲和系的显性-隐性层次一致。在显性系中存在隐性系以外的附加序列，如类转座子序列、重复序列和细胞器基因组类序列片段。由此可见，三裂甘蓝自交不亲和性较高层级的品系是由隐性品系发展而来。较少

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Physical size of the S locus region defined by genetic recombination and genome sequencing in Ipomoea trifida, Convolvulaceae

DOI：
10.1007/s00497-007-0044-x
发表时间：
2007-06-01
期刊：
SEXUAL PLANT REPRODUCTION
影响因子：
0
作者：
Rahman, Md. Habibur;Tsuchiya, Tohru;Kowyama, Yasuo
通讯作者：
Kowyama, Yasuo

Genomic sequence polymorphism and dominance-recessive relationship between several S haplotype of Ipomoea trifida

三裂蕹几种S单倍型的基因组序列多态性及显性-隐性关系

DOI：
发表时间：
2007
期刊：
影响因子：
0
作者：
Kowyama;Y.;Rahman;H.;Takakita;H.;Taniguchi;O.;Kakeda;K.;Tsuchiya;T
通讯作者：
T

Expression of stigma- and anther-specific genes located in the S locus region of Ipomoea trifida.

位于三裂薯 S 基因座区域的柱头和花药特异性基因的表达。

DOI：
发表时间：
2007
期刊：
Sexual Plant Reproduction 20・2(online published)(印刷中)
影响因子：
0
作者：
Rahman;M.H.;et al.
通讯作者：
et al.

サツマイモ野生種のSハプロタイプ間におけるゲノム配列多型性と優劣性関係

野生甘薯S单倍型基因组序列多态性及优劣关系

DOI：
发表时间：
2007
期刊：
影响因子：
0
作者：
Md.Habibur Rahman;Mina Uchiyama;Masashi Kuno;Natsuko Hirashima;Keita Suwabe;Tohru Tsuchiya;Yasuaki Kagaya;Issei Kobayashi;Katsuyuki Kakeda;Yasuo Kowyama;神山康夫・M. Rahman・高北英治・谷口理・掛田克行・土屋亨
通讯作者：
神山康夫・M. Rahman・高北英治・谷口理・掛田克行・土屋亨

Molecular genetics of sporophytic self-incompatibility in Ipomoea, a member of the Convolvulaceae

旋花科植物蕹菜孢子体自交不亲和性的分子遗传学