Characterization of molecular mechanisms of sporophytic self-incompatibility in Ipomoea trifida

三裂蕹孢子体自交不亲和的分子机制表征

基本信息

批准号：
18580004
负责人：
TSUCHIYA Tohru
金额：
$ 2.49万
依托单位：
Mie University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18580004/
关键词：
Self-incomoatibility Wild type Sweet potato Ioomoea trifida Ipomoea trrfrda

项目摘要

To identify genes correlated to self-incompatibility(S-genes)of wild type sweet potato(Ipomoea trifida), genomic sequences of S-locus from S1, 53, S10, S29 self-incompatible lines and Sc, the self-compatible line, were isolated and sequenced.By the expression analysis and sequence polymorphism, several S-gene candidates were identified ; AB1 to AB4 as male S-gene candidates and SE1 to SE2 and SEA as female S-gene candidates. Among these genes, AB2, A83, SE2 and SEA were found from all analyzed S-locus sequence. In the Sc S-haplotype, however, some mutations were observed in the promoter sequence and gene itself in some of the S-gene candidates. These mutations may contribute the Sc-haplotype as self-compatible.To determine spatial and temporal expression of these four S-gene candidates, in situ hybridization analyses were carried out. In situ analyses indicated that all of the male part S-candidate genes were expressed in the tapetum at early developmental stage, and all of the female … More part S-candidate genes were expressed in the papilla cells of stigma at mature stage. These results indicated that all S-candidate genes share a necessary and sufficient condition for S-genes.Comparison between the genomic sequences of five S-haplotypes indicated that the existence of a regions which have highly sequence polymorphisms, designated as S-locus diversed regions(SDRs). Within this SDRs, three S-candidate genes were located, which are AB2, AB3 and SEA, therefore, these gene products might play key roles in the self-incompatibility reaction.Furthermore, comparison of the length of the SDRs from self-incompatible lines indicated that SDR length is longest in the most dominant S-haplotype and is shortest in the most recessive line, and the order of the SDR length is consistent with the dominance-recessive hierarchy of the self-incompatibility. In the dominance line have additional sequence to recessive line, such as transposon-like sequences, repetitive sequences, and fragments of organelle genome-like sequences. From this observation, it was considered that the lines at the higher hierarchy in the self-incompatibility of Ipomoea trifida was developed from recessive line. Less

鉴定与野生型红薯（ipomoea trifida）的自我兼容性（S-Genes）相关的基因，来自S1、53，S10，S10，S10，S10，S10，S10，S10，S10，S10，S10，S10，S10，S10，S10，S10，S29的自我不相容线的基因组序列，自我兼容线，自我兼容线，分离且序列。通过表达分析和序列polymorphistation，几个S-GELES，几个是鉴定的，是鉴定的; AB1至AB4作为男性S基因候选人，SE1至SE2和SE2和SEA作为女性S-Gene候选者。在这些基因中，从所有分析的S-Ocus序列中发现了AB2，A83，SE2和SEA。然而，在SC S-Haplotype中，在启动子序列中观察到了一些突变，并且在某些S-Gene候选物中观察到了基因本身。这些突变可能将SC-HAPLOTY型作为自兼容。为了确定这四个S基因候选物的空间和临时表达，进行了原位杂交分析。原位分析表明，所有雄性部分s-候选基因均在早期发育阶段的tapetum中表达，所有女性……更多的s-候选基因在成熟阶段的柱头乳头细胞中表达。这些结果表明，所有S候选基因具有S-genes.com的必要条件。在该SDR中，位于AB2，AB3和SEA的三个S候选基因，因此，这些基因产品可能在自兼容性反应中起关键作用。Furthermore，比较自我不合别线的SDR长度的比较表明，SDR的长度表明SDR的长度在最优势的S-Haplotype和最短时间内是最大的，并且SDRE的长度是一行，并且是SDR的长度，并且是SDR的长度，并且是SDR的长度，并且是SDR的长度，并且是SDR的长度，并且是SDR的长度，并且SDR的长度是一行，并且是SDR的长度，并且是SDR的长度。自我兼容性的统治层次结构。在优势线中，有其他序列对隐性线的序列，例如类似转座子的序列，重复序列和细胞器基因组样序列的片段。从这一观察结果，可以从隐性线发展依赖于ipomoea trifida的自我兼容性的较高层次结构的线。较少的

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Physical size of the S locus region defined by genetic recombination and genome sequencing in Ipomoea trifida, Convolvulaceae

DOI：
10.1007/s00497-007-0044-x
发表时间：
2007-06-01
期刊：
SEXUAL PLANT REPRODUCTION
影响因子：
0
作者：
Rahman, Md. Habibur;Tsuchiya, Tohru;Kowyama, Yasuo
通讯作者：
Kowyama, Yasuo

Genomic sequence polymorphism and dominance-recessive relationship between several S haplotype of Ipomoea trifida

三裂蕹几种S单倍型的基因组序列多态性及显性-隐性关系

DOI：
发表时间：
2007
期刊：
影响因子：
0
作者：
Kowyama;Y.;Rahman;H.;Takakita;H.;Taniguchi;O.;Kakeda;K.;Tsuchiya;T
通讯作者：
T

サツマイモ野生種のSハプロタイプ間におけるゲノム配列多型性と優劣性関係

野生甘薯S单倍型基因组序列多态性及优劣关系

DOI：
发表时间：
2007
期刊：
影响因子：
0
作者：
Md.Habibur Rahman;Mina Uchiyama;Masashi Kuno;Natsuko Hirashima;Keita Suwabe;Tohru Tsuchiya;Yasuaki Kagaya;Issei Kobayashi;Katsuyuki Kakeda;Yasuo Kowyama;神山康夫・M. Rahman・高北英治・谷口理・掛田克行・土屋亨
通讯作者：
神山康夫・M. Rahman・高北英治・谷口理・掛田克行・土屋亨

Molecular genetics of sporophytic self-incompatibility in Ipomoea, a member of the Convolvulaceae

旋花科植物蕹菜孢子体自交不亲和性的分子遗传学