Breakdown of Self- and Cross-incompatibility of Sweet Potato

栽培甘薯（Ipomoea batatas）物种/栽培种具有杂交不亲和性机制，因此不能实现有效的杂交育种。甘薯的这种杂交不亲和性是建立在栽培甘薯二倍体野生种（三裂叶甘薯）自交不亲和系统的基础上的，因此对三裂叶甘薯自交不亲和性的研究，将直接反映甘薯杂交不亲和性的解除。本研究采用生物测定法，将雄性S基因产物涂抹在授粉后的柱头表面，对三裂叶冬青的雄性部分S基因进行了鉴定，并对雌性部分S基因进行了候选基因的鉴定。对这些S基因和S候选基因的功能进行了同源性检索，并与网上的DNA和蛋白质数据库进行了比对。然而，通过转基因和非转基因的方法都不能解决甘薯的杂交和自交不亲和性。

Cultivated sweet potato (Ipomoea batatas) species/cultivars carry cross-incompatibility mechanism; therefore efficient cross breeding is not achieved. This cross-incompatibility of I.batatas is based on the self-incompatibility system of wild type diploid species (I.trifida) which related to cultivated sweet potato, so the research on self-incompatibility of I.trifida, will directly reflect on the breakdown of cross-incompatibility of I.batatas. In this research, we determine the male-part S-gene of I.trifida by bioassay, in which applying male-S-gene product on the surface of stigma upon pollination, and we identified female-S-candidate genes. Function of these S-gene and S-candidate genes were verified by homology search against DNA and protein databases on the net. However, Breakdown the cross- and self-incompatibility of sweet potato is not achieved by GM and non-GM ways.