Study of sel/non-selfrecognition system in sel/cross incompatibility of wild type and cultivated sweet potato

野生型与栽培甘薯自/杂交不亲和性中的自/非自识别系统研究

基本信息

  • 批准号:
    20580004
  • 负责人:
  • 金额:
    $ 3.08万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2008
  • 资助国家:
    日本
  • 起止时间:
    2008 至 2010
  • 项目状态:
    已结题

项目摘要

Wild type sweet potato (Ipomoea trifida) has sporophytic self-incompatibility (SSI) system, but this system is not based on the SPII/SCR and SRK system, which is observed in Brassica. To identify S genes of Ipomoea SSI system, S-locus genomic regions from several S-haplotypes, including self-compatible (Sc) mutant, were isolated and analyzed. According to the shotgun sequencing of S-locus genomic regions from four S-haplotypes, the length of the S-locus genomic region was parallel to the dominant-recessive hierarchy of S-genes ; longer in dominants, shorter in recessives. In the Sc mutant line, all S-candidate genes ; AB2 for male, and SE2 and SEA for female ; were located in the Sc-S-locus, however, AB2 and SEA genes were duplicated, and two SEA genes indicated ectopic expression in the male reproductive organs. It is possible to consider that this ectopic expression of SEA genes make Sc line as self-compatible. S-candidate gene analyses of wild type (I.trifida) and cultivated (I.batatas) indicated that S_3 haplotype ofLtrifzda is conserved in the I.batatas. This result indicates that the self/cross-incompatibility in cultivated sweet potato is based on the same molecular mechanism as in the self-incompatibility of I.trifida.
野生型红薯(ipomoea trifida)具有孢子型自我兼容性(SSI)系统,但该系统并不基于SPII/SCR和SRK系统,该系统在Brassica中观察到。为了鉴定依属SSI系统的S基因,分离和分析了来自几种S-型型型(包括自兼容(SC)突变体)的S-Ocus基因组区域。根据来自四种S-型型的S-核心基因组区域的shot弹枪测序,S-Ocus基因组区域的长度与S-Genes的主要不必要的层次平行。占主导地位的时间更长,在隐性中较短。在SC突变线中,所有S candidate基因; AB2为男性,女性的SE2和SEA;然而,位于SC-S-cocus中,AB2和SEA基因被复制,并且两个海基因表明男性生殖器官的异位表达。可以考虑,这种海基因的异位表达使SC线成为自我兼容。野生型(i.trifida)和培养(i.batatas)的S候选基因分析表明,在i.batatas中保守了ltrifzda的S_3单倍型。该结果表明,栽培红薯中的自我/跨兼容性基于与i.trifida的自我不相容性相同的分子机制。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Family 17 and 28 Carbohydrate-Binding Modules Discriminate Different Cell Wall Sites in Sweet Potato Roots
家族 17 和 28 碳水化合物结合模块区分甘薯根中的不同细胞壁位点
Sexual reproduction is the default mode in apomictic Hieracium subgenus Pilosella where two dominant loci function to enable apomixes.
有性生殖是无融合生殖的 Hieracium 亚属 Pilosella 的默认模式,其中两个显性基因座发挥作用以实现无融合生殖。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Koltunow A.;Johnson S.;Rodrigues J.;Okada T.;Yingkao H.;Tsuchiya T.;Wilson S.;Fletcher P.;Ito K.;Suzuki G.;Mukai Y.;Fehrer J.;Bicknell R.
  • 通讯作者:
    Bicknell R.
Self-Incompatibility in Flowering Plants-Evolution, Diversity and Mechanisms (V. E. Franklin-Tong ed. ) Chapter 12 "Molecular Genetics of Sporophytic Self-Incompatibility in Ipomoea, a Member of the Convolvulaceae"
开花植物的自交不亲和性 - 进化、多样性和机制(V. E. Franklin-Tong 编辑)第 12 章“旋花科植物甘薯孢子体自交不亲和性的分子遗传学”
  • DOI:
  • 发表时间:
    2008
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Y. Kowyama;T. Tsuchiya;K. Kakeda
  • 通讯作者:
    K. Kakeda
Bicknell R.Sexual reproduction is the default mode in apomictic Hieracium subgenus Pilosella where two dominant loci function to enable apomixes.
Bicknell R.有性生殖是无融合生殖的 Hieracium 亚属 Pilosella 的默认模式,其中两个显性基因座发挥无融合生殖的功能。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Koltunow A.;Johnson S.;Rodrigues J.;Okada T.;Yingkao H.;Tsuchiya T.;Wilson S.;Fletcher P.;Ito K.;Suzuki G.;Mukai Y.;Fehrer J.
  • 通讯作者:
    Fehrer J.
Handbook of New Technologies for Genetic Improvement of Legumes (P. B. Kirtied. ). Chapter 31 "Gene Expression Analysis with DNA Array Technology in Legumes"
豆类遗传改良新技术手册(P. B. Kirtied.)。
  • DOI:
  • 发表时间:
    2008
  • 期刊:
  • 影响因子:
    0
  • 作者:
    M. Watanabe;M. Endo;H. Hakozaki;H. Masuko;J-I. Park;M. Kawagishi-Kobayashi;T. Tsuchiya
  • 通讯作者:
    T. Tsuchiya
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TSUCHIYA Tohru其他文献

TSUCHIYA Tohru的其他文献

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{{ truncateString('TSUCHIYA Tohru', 18)}}的其他基金

New stage in the study of a unique non-model cyanobacterium that can use far-red light for photosynthesis by genome editing
通过基因组编辑利用远红光进行光合作用的独特非模型蓝藻研究进入新阶段
  • 批准号:
    18K05389
  • 财政年份:
    2018
  • 资助金额:
    $ 3.08万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Molecular genetic analysis of the cyanobacterium that can utilize far-red light for photosynthesis using chlorophyll d
利用叶绿素 d 利用远红光进行光合作用的蓝藻的分子遗传学分析
  • 批准号:
    24658080
  • 财政年份:
    2012
  • 资助金额:
    $ 3.08万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Breakdown of Self- and Cross-incompatibility of Sweet Potato
甘薯自交不亲和性和杂交不亲和性的解析
  • 批准号:
    23580005
  • 财政年份:
    2011
  • 资助金额:
    $ 3.08万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Elucidating the fundamental questions "photochemical reaction and its site" using unique cyanobacteria
利用独特的蓝细菌阐明“光化学反应及其场所”的基本问题
  • 批准号:
    22370017
  • 财政年份:
    2010
  • 资助金额:
    $ 3.08万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Characterization of molecular mechanisms of sporophytic self-incompatibility in Ipomoea trifida
三裂蕹孢子体自交不亲和的分子机制表征
  • 批准号:
    18580004
  • 财政年份:
    2006
  • 资助金额:
    $ 3.08万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

相似海外基金

Identification of QTLs involved in root thickness in the wild sweetpotato
野生甘薯根粗度QTL的鉴定
  • 批准号:
    20K15505
  • 财政年份:
    2020
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  • 项目类别:
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Identification of S genes of Ipomoea trifida, a wild relative of sweetpotato
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  • 批准号:
    26450002
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Breakdown of Self- and Cross-incompatibility of Sweet Potato
甘薯自交不亲和性和杂交不亲和性的解析
  • 批准号:
    23580005
  • 财政年份:
    2011
  • 资助金额:
    $ 3.08万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Elucidation of the molecular genetic mechanism involved in theself/non-self recognition of self-incompatibility in a plant family, Convolvulaceae
旋花科植物自交/非自交不亲和性识别的分子遗传机制的阐明
  • 批准号:
    19380005
  • 财政年份:
    2007
  • 资助金额:
    $ 3.08万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
サツマイモ野生種の胞子体型自家不和合性における新規認識機構の分子的解明
野生甘薯孢子体自交不亲和性新识别机制的分子阐明
  • 批准号:
    15780003
  • 财政年份:
    2003
  • 资助金额:
    $ 3.08万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
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