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Function of Mugineic acid in absorption and translocation of micro elements in graminaceous plants

麦根酸在禾本科植物微量元素吸收和转运中的作用

基本信息

批准号：
18580058
负责人：
KAWAI Shigenao
金额：
$ 2.49万
依托单位：
Iwate University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18580058/
关键词：
Arsenic Nickel Mugineic acid Hydroponical Culture Barley Xylem Sap Formation of Chelate Vesicle of Plasma Membrane 吸収原子吸光光度計シオチガヤ

项目摘要

1. Relationship between release of mugineic acid (MA) and toxicity of metal micronutrient (Ni) Radioactive ^<63>Ni were fed together with MA to the barley grown hydroponically and activation of Ni absorption by MA was concluded. Absorption of ^<59>Fe was repressed by coexisting Ni in the presence of MA. Furthermore, Absorption of ^<63>Ni was not repressed by coexisting Fe. The results indicated that Ni is preferentially absorbed to Fe in the form of MA complex.2. Relationship release of MA and harmful elements (Cd, As, Co) In order to confirm that the cause of As-induced chlorosis is Fe-deficiency in barley grown hydroponically, plants were grown in the medium with the elevated Fe concentration. As a result, As-induced chlorosis was alleviated. The main cause of As-induced chlorosis was concluded to be Fe-deficiency.3. Amount of translocated micronutrient in xylem tube and effect of fed MA Manganese, Zn, Cu and with excess concentrations were fed to the barley grown hydroponically with … More or without MA for 3 hours in the afternoon, and in subsequent hours the concentrations of the metals in xylems sap was measured. The results showed that in normal plants only the concentration of Fe was increased by feeding of MA, but in Mn, Zn, or Cu toxic plants MA could increase of Cu or Zn concentration of xylem sap. It was suggested that in the Cu or Zn toxic plants released MA might aggravate Cu or Zn-induced Fe-deficiency.4. Clarification of the form of MA-metals chelates By the analysis of NMR, it was concluded that molar ratio of MA and metals, such as Fe^<3+>, Cu^<2+>, Zn^<2+>, Mn^<2+>, Ni^2, when chelates were formed, was 1:1. Furthermore, the order of strength of formation was Cu^<2+>>Fe^<3+>>Ni^<2+>>Mn^<2+>>Zn^<2+>.5. Examination of absorption of trace elements in the isolated vesicles of plasma membrane In the experiment with isolated vesicles of plasma membrane prepared from roots of Fe-deficient barely, absorption of Cd was repressed by the presence of Cu or Fe. The repression effect of Cu was larger than that of Fe. It was considered that it may be attributed to characteristic of the vesicles of plasma membrane to Fe and Cu. Less

1.麦根酸(MA)的释放与金属微量营养素(Ni)的毒性之间的关系将放射性^ 63 Ni与MA一起喂入水培大麦中，并得出MA对Ni吸收的激活。在MA存在下， 59 Fe的吸收被共存的Ni抑制。此外，共存的Fe不会抑制 63 Ni的吸收。结果表明，Ni以MA配合物的形式优先被Fe吸附。 2． MA 释放与有害元素（Cd、As、Co）的关系为了证实水培大麦中砷引起的失绿的原因是缺铁，将植物培养在铁浓度升高的培养基中。结果，As引起的萎黄病得到缓解。推测As引起失绿的主要原因是缺铁。 3．木质部管中转位微量营养素的量以及饲喂MA的影响将锰、锌、铜和过量浓度的锰、锌、铜饲喂到水培大麦中，在下午3小时内添加或不添加MA，并在随后的几个小时内测量木质部汁液中金属的浓度。结果表明，在正常植物中，饲喂MA仅增加了Fe的浓度，但在Mn、Zn或Cu有毒植物中，MA可以增加木质部汁液中的Cu或Zn浓度。提示Cu、Zn有毒植物释放的MA可能会加重Cu、Zn引起的铁缺乏症。 4． MA-金属螯合物形态的澄清通过NMR分析，得出MA与Fe^<3+>、Cu^<2+>、Zn^<2+>、Mn^<2+>、Ni^2等金属形成螯合物时的摩尔比为1:1。此外，形成强度的顺序为Cu^<2+>>Fe^<3+>>Ni^<2+>>Mn^<2+>>Zn^<2+>.5。质膜分离囊泡中微量元素吸收的检测在用几乎不缺铁的根部制备的质膜分离囊泡的实验中，Cd 的吸收因Cu 或Fe 的存在而受到抑制。 Cu的抑制作用大于Fe的抑制作用。认为这可能归因于质膜囊泡对Fe和Cu的特性。较少的