Studies on the properties of toxins containedin boxfish as the causative agents in food poisonings

箱鲀所含毒素作为食物中毒致病因子的特性研究

• 批准号: 18580204
• 负责人: ASAKAWA Manabu
• 金额: $ 2.47万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18580204/
• 关键词: boxfish; Ostreopsis siamensis; dinoflagellate; myoglobinuria; palytoxin; delayed haemolytic activity; parrotfish; LC; MS; g-ストロファンチン; ESM培地; シガテラ毒

The purpose of this is to daffy the properties of the toxins contained in boxfish Ostracion cubicus as the causative agents in food poisonings due to ingestion of them. The toxins as the causative agents was isolated from the boxfish and the specimens of dinoflagellate Ostreopsis siamensis apossible origin of their toxins using some chromatography. After checking their toxicities of them using MY male mice, their chemical properties was examined and cleared using mainly LC/MS. The crude toxin from the box fish O.cubicus and O.siamensis was extracted, and was tasted. The strains of O.siamensis isolated in June, 2005, Nagasaki Prefecture showed toxicity to mice. The mice injected with 1-butanol layer from them showed the common symptoms of convulsion, drowsiness and collapse, and died within 48 h. Toxins isolated showed delayed haemolytic activity with mouse and human erythrocytes, which was inhibited by ouabain. After being injected with toxins, the serum creatine phosphokinase levels of mice were found to be elevated. By LC/MS analysis, the toxin content of this strain of O.siamensis contain was estimated 1.7 pg/cell as PTX.From these results, it can be strongly postulated that the dinoflagellate O.siamensis is the origin of palytoxin like substance which is sequestered by boxfish through food web.

其目的是为了确定黄箱鲀中所含毒素的性质，因为它们是由于摄入黄箱鲀而引起食物中毒的病原体。从箱鲀和甲藻Ostreopsis siamensis标本中分离出致病毒素，并对其可能来源进行了初步鉴定。在对MY雄性小鼠进行毒性试验后，主要采用LC/MS法对毒素的化学性质进行了检测和分析。2005年6月在长崎县分离的暹罗圆线虫对小鼠有毒性。注射正丁醇层的小鼠出现惊厥、嗜睡、虚脱等常见症状，48 h内死亡。分离的毒素表现出延迟溶血活性与小鼠和人的红细胞，这是抑制哇巴因。毒素注射后，小鼠血清肌酸磷酸激酶水平升高。经LC/MS分析，该菌株的毒素含量为1.7pg/cell（PTX），推测该毒素可能来源于甲藻O.siamensis。

项目成果

セブ島オウギガニ科Demania属カニの毒性について

关于宿务岛 Demania 科 Demania 属螃蟹的毒性

• 发表时间: 2007
• 作者: Manabu;Asakawa;浅川 学
• 通讯作者: 浅川 学

Analysis method of palytoxin using high performance liquid chromatography

海藻毒素的高效液相色谱分析方法

• 发表时间: 2007
• 期刊: Bull.Shikoku Univ. (B) 24
• 作者: Takefumi;Sagara
• 通讯作者: Sagara

Accumulation of tetrodotoxin (TTX) in Pseudocal igus fugu, a parasitic copepod from panther Takifugu pardalis but without vertical transmission - using an immunoenzymatic technique

河豚毒素 (TTX) 在拟东方鲀（一种来自美洲豹 Takifugu pardalis 的寄生桡足类）中的积累，但没有垂直传播 - 使用免疫酶技术

• 发表时间: 2006
• 期刊: Toxicon 48
• 作者: Katsutoshi;Ito;Koichi Ikeda
• 通讯作者: Koichi Ikeda

フィリピン産オウギガニ科カニDemania cultripesの毒性と毒成分ついて

菲律宾苋菜的毒性及有毒成分

• 发表时间: 2008
• 作者: Katsutoshi;Ito;Koichi Ikeda;Katsutoshi Ito;鶴田慎太郎
• 通讯作者: 鶴田慎太郎

Toxicity of Xanthid crab from Sebu Island, Philippines

菲律宾塞布岛的 Xanthid 蟹的毒性

• 发表时间: 2007
• 作者: Manabu;Asakawa
• 通讯作者: Asakawa