Studies on the properties of toxins containedin boxfish as the causative agents in food poisonings

箱鲀所含毒素作为食物中毒致病因子的特性研究

• 批准号: 18580204
• 负责人: ASAKAWA Manabu
• 金额: $ 2.47万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18580204/
• 关键词: boxfish; Ostreopsis siamensis; dinoflagellate; myoglobinuria; palytoxin; delayed haemolytic activity; parrotfish; LC; MS; g-ストロファンチン; ESM培地; シガテラ毒

The purpose of this is to daffy the properties of the toxins contained in boxfish Ostracion cubicus as the causative agents in food poisonings due to ingestion of them. The toxins as the causative agents was isolated from the boxfish and the specimens of dinoflagellate Ostreopsis siamensis apossible origin of their toxins using some chromatography. After checking their toxicities of them using MY male mice, their chemical properties was examined and cleared using mainly LC/MS. The crude toxin from the box fish O.cubicus and O.siamensis was extracted, and was tasted. The strains of O.siamensis isolated in June, 2005, Nagasaki Prefecture showed toxicity to mice. The mice injected with 1-butanol layer from them showed the common symptoms of convulsion, drowsiness and collapse, and died within 48 h. Toxins isolated showed delayed haemolytic activity with mouse and human erythrocytes, which was inhibited by ouabain. After being injected with toxins, the serum creatine phosphokinase levels of mice were found to be elevated. By LC/MS analysis, the toxin content of this strain of O.siamensis contain was estimated 1.7 pg/cell as PTX.From these results, it can be strongly postulated that the dinoflagellate O.siamensis is the origin of palytoxin like substance which is sequestered by boxfish through food web.

这样做的目的是使Cobicus中包含的毒素的特性作为食物中毒的因果剂。毒素是从盒子中分离出来的因果剂，并使用一些色谱法分离出其毒素的鞭毛藻的标本。使用我的雄性小鼠检查它们的毒性后，使用LC/MS检查并清除其化学特性。提取了盒子中的原油毒素和o.siamensis，并品尝了。 siamensis的菌株于2005年6月分离出来，长崎县对小鼠的毒性显示出毒性。从中注入1-丁醇层的小鼠显示出抽搐，嗜睡和崩溃的常见症状，并在48小时内死亡。分离的毒素显示出用小鼠和人类红细胞延迟溶血活性，瓦巴因抑制了。注射毒素后，发现小鼠的血清肌酸磷酸激酶水平升高。通过LC/MS分析，含有ptx的O.siamensis菌株的毒素含量估计为1.7 pg/cell。从这些结果中，可以强烈假设鞭毛粉O.siamensis是palytoxin类似物质的起源，它是由Boxfish通过Food Web隔离的。

项目成果

セブ島オウギガニ科Demania属カニの毒性について

关于宿务岛 Demania 科 Demania 属螃蟹的毒性

• 发表时间: 2007
• 作者: Manabu;Asakawa;浅川 学
• 通讯作者: 浅川 学

Accumulation of tetrodotoxin (TTX) in Pseudocal igus fugu, a parasitic copepod from panther Takifugu pardalis but without vertical transmission - using an immunoenzymatic technique

河豚毒素 (TTX) 在拟东方鲀（一种来自美洲豹 Takifugu pardalis 的寄生桡足类）中的积累，但没有垂直传播 - 使用免疫酶技术

• 发表时间: 2006
• 期刊: Toxicon 48
• 作者: Katsutoshi;Ito;Koichi Ikeda
• 通讯作者: Koichi Ikeda

Analysis method of palytoxin using high performance liquid chromatography

海藻毒素的高效液相色谱分析方法

• 发表时间: 2007
• 期刊: Bull.Shikoku Univ. (B) 24
• 作者: Takefumi;Sagara
• 通讯作者: Sagara

Isolation and characterization of bacteria from the copepod Pseudocaligus fugu ectoparasitic on the panther puffer Takifugu pardalis with the emphasis on TTX

• DOI: 10.1016/j.toxicon.2007.06.021
• 发表时间: 2007-11-01
• 期刊: TOXICON
• 影响因子: 2.8
• 作者: Maran, B. A. Venmathi;Iwamoto, Emi;Boxshall, Geoffrey A.
• 通讯作者: Boxshall, Geoffrey A.

Toxicity of Xanthid crab from Sebu Island, Philippines

菲律宾塞布岛的 Xanthid 蟹的毒性

• 发表时间: 2007
• 作者: Manabu;Asakawa
• 通讯作者: Asakawa