Studies on the properties of toxins containedin boxfish as the causative agents in food poisonings

这样做的目的是为了研究方头鱼中所含毒素的性质，这些毒素是由于摄取这些毒素而导致食物中毒的病原体。利用层析技术，从方头鱼和甲藻标本中分离出可能为其毒素来源的毒素。在用我的雄性小鼠检测了它们的毒性后，主要用LC/MS对它们的化学性质进行了检测和清除。从箱鱼中提取了粗毒素，并进行了品尝。2005年6月从长崎县分离的亚洲稻瘟病菌对小鼠有一定的毒性。注射1-丁醇层的小鼠出现惊厥、嗜睡和虚脱的常见症状，48h内死亡。分离的毒素与小鼠和人的红细胞具有延迟的溶血活性，哇巴因对此有抑制作用。注射毒素后，小鼠血清肌酸磷酸激酶水平升高。经LC/MS分析，该菌株的毒素含量为1.7pg/cell为PTX。由此推测，甲藻毒素类物质是拟孢子虫毒素物质的来源，这种物质是箱鱼通过食物网隔离的。

The purpose of this is to daffy the properties of the toxins contained in boxfish Ostracion cubicus as the causative agents in food poisonings due to ingestion of them. The toxins as the causative agents was isolated from the boxfish and the specimens of dinoflagellate Ostreopsis siamensis apossible origin of their toxins using some chromatography. After checking their toxicities of them using MY male mice, their chemical properties was examined and cleared using mainly LC/MS. The crude toxin from the box fish O.cubicus and O.siamensis was extracted, and was tasted. The strains of O.siamensis isolated in June, 2005, Nagasaki Prefecture showed toxicity to mice. The mice injected with 1-butanol layer from them showed the common symptoms of convulsion, drowsiness and collapse, and died within 48 h. Toxins isolated showed delayed haemolytic activity with mouse and human erythrocytes, which was inhibited by ouabain. After being injected with toxins, the serum creatine phosphokinase levels of mice were found to be elevated. By LC/MS analysis, the toxin content of this strain of O.siamensis contain was estimated 1.7 pg/cell as PTX.From these results, it can be strongly postulated that the dinoflagellate O.siamensis is the origin of palytoxin like substance which is sequestered by boxfish through food web.