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Risk of Listeria monocytogenes in seafood products

海鲜产品中单核细胞增多性李斯特菌的风险

基本信息

批准号：
18580203
负责人：
KIMURA Bon
金额：
$ 2.49万
依托单位：
Tokyo University of Marine Science and Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18580203/
关键词：
Listeria monocytogenes ready-to-eat seafood Listeria monocytogenes

项目摘要

Listeria monocytogenes contamination of certain marine food products that are eaten raw, such as cod ovum and salmon roe, has recently been reported in Japan and has also been confirmed in our recent research. In the present study, we aimed to gain a basic understanding of the risk of L. monocytogenes contamination of marine food products by investigating its distribution and detection in marine food products that are eaten raw and by investigating the properties of L. monocytogenes isolates from food products, as well as its proliferation and viability in marine food products.In our 2006 study, We conducted a comprehensive investigation of the distribution of L. monocytogenes and isolated strains in commercially available marine food products, including cod ovum, salmon roe, and negitoro (tuna and green onion). Furthermore, for positive samples detected using the miniVIDAS method (an official AOAC method based on immunology methods) we performed a smear test for the detection of L. monocytogenes using differential media followed by identification of typical colonies(confirmed by API and PCR. In addition, we determined the serotypes and basic genetic properties of isolates.In our 2007 research, we continued our detailed investigation of the virulence genes of strains. Along with developing a method for typing and identifying strains, such as the development of a simple genetic typing method for L. monocytogenes based on the investigation of virulence gene inIA, we obtained basic data on the proliferation of L. monocytogenes in marine food products and under conditions for food production, such as a proliferation test for negitoro at a storage temperature of 5°C, and .evaluation of viability through evaluation of biofilm-forming ability in the food processing factory.Based on our previous results, we were gain basic knowledge on the risk of L. monocytogenes contamination in marine food products.

日本最近报告了某些生吃的海洋食品（如鳕鱼卵和鲑鱼卵）受到单核细胞增生李斯特菌污染的情况，我们最近的研究也证实了这一点。在本研究中，我们的目的是获得一个基本的了解的风险L。通过调查海洋食品中单核细胞增生性李斯特菌的分布和检测情况以及调查其特性，在2006年的研究中，我们对食品中的单核细胞增生李斯特菌的分布进行了全面的调查。在市售海洋食品中，包括鳕鱼卵、鲑鱼卵和金枪鱼（金枪鱼和绿色洋葱）中的单核细胞增多症和分离菌株。此外，对于使用miniVIDAS方法（一种基于免疫学方法的官方AOAC方法）检测到的阳性样本，我们进行了涂片检测以检测L。单核细胞增多症，随后鉴定典型菌落（通过API和PCR确认）。此外，我们确定了分离株的血清型和基本遗传特性。在2007年的研究中，我们继续对菌株的毒力基因进行了详细的调查。沿着发展一种用于分型和鉴定菌株的方法，例如发展一种用于L.通过对单核细胞增生李斯特菌毒力基因的研究，获得了该菌增殖的基础数据。在海洋食品和食品生产条件下，例如在5°C的储存温度下对L.i.toro进行增殖试验，以及通过评价食品加工厂的生物膜形成能力来评价存活力。海产食品中的单核细胞增多症。