Search for the specific gene region in Silkie chicken

乌骨鸡(Gallus Gallus)在中医学中发挥了重要作用，但与黑色素的特定外观和沉积相关的基因尚不清楚。以乌骨鸡和白来航鸡为材料，采用聚合酶链式反应消减技术构建了3个基因组消减文库。我们对这些文库中的178个克隆进行了测序，发现了86个独特的克隆。以丝羽乌骨鸡和白来航鸡为材料，对PCR-RFLP体系中的引物进行了检测。在13对引物中，乌骨鸡与白来航鸡之间存在多态性。本研究以53只乌骨鸡、19只日本土鸡和6只商品鸡为材料，研究了丝羽乌骨鸡催乳素基因启动子区24个碱基的插入情况。46只乌骨鸡具有Del/Del基因，7只乌骨鸡具有In/Del基因，但…有更多的乌骨鸡具有In/In基因。另有11只鸡、8只鸡和6只鸡分别为Del/Del、In/Del和In/In。用2×3表进行卡方检验，乌骨鸡与其他鸡的基因频率差异极显著(P&lt；0.01)。这些结果表明，乌骨鸡催乳素基因启动子区24个碱基插入的基因型频率与其他鸡有显著差异。乌骨鸡在中医学中起着重要的作用，但乌骨鸡多产蛋，多产蛋。催乳素基因被认为是母鸡就巢的开始和维持所必需的。与红色丛林鸡相比，白色来航鸡的催乳素基因启动子区域存在24bp的插入，缺失24bp的插入将维持催乳素启动子的正常功能。本研究以产蛋用丝羽乌骨鸡第四代群体为材料，对其24个碱基的插入进行了基因分型，并探讨了该插入基因与生产性能的关系。从大分县农林水产科学研究中心畜牧研究所选育的第四代乌骨鸡群体中采集69只雄性乌骨鸡和242只雌性乌骨鸡的血样。从血样中提取基因组DNA，用聚合酶链式反应扩增催乳素基因启动子片段。将扩增产物在3.0%琼脂糖凝胶上进行基因分型。本研究共发现216只德尔/德尔乌骨鸡，26只印度/戴尔乌骨鸡，未发现印度/印度乌骨鸡。用方差分析分析了该基因插入位点的基因型与生产性能的关系，而与生长性状和产蛋量的关系不显著。In/Del乌骨鸡的151d-450d产蛋率分布峰值高于De/Del乌骨鸡。较少

Silkie chicken (Gallus gallus) have played an important role in traditional Chinese Medicine, however the genes associated with the specific appearance and deposition of the melanin pigment are unknown. The three genomic subtraction libraries were constructed using PCR subtraction method between a Silkie chicken and a White Leghorn chicken. We sequenced 178 clones of these libraries and found the 86 unique clones. The primer pairs for PCR-RFLP system were examined for the Silkie chicken and the White Leghorn chicken. The polymorphism between Silkie chicken and White Leghorn was found in the thirteen primer pairs. The 5% level significant differences of allele frequency between Silkie chicken and the other breeds were found in two PCR-RFLP primer pairs.The 24-bp insertion of the promoter region of prolactin gene was studied for 53 Silkie fowls, 19 Japanese native chickens and 6 commercial chickens. Forty-six Silkie fowls have genotype Del/Del, 7 Silkie fowls have genotype In/Del, howeve … More r no Silkie fowls have genotype In/In. On the other hand, 11, 8 and 6 birds of other chickens showed genotypes Del/Del, In/Del and In/In, respectively. The chi-squared test using 2x3 table showed the significant difference (P<0.01) of the genotype frequencies between Silkie fowls and other chickens. These results show the significant difference of the genotype frequencies for the 24-bp insertion of the promoter region of prolactin gene between Silkie fowls and other chickens.Silkie chicken (Gallus gallus) have played an important role in traditional Chinese Medicine, however broodiness is often observed and egg production ratio is very small in Silkie chicken. It is thought that prolactin gene is an essential for the start and maintain of broodiness in hens. The 24-bp insertion is found in the promoter region of prolactin gene of White Leghorn as compare with Red Jungle fowl and the absence of the 24-bp insertion would maintain normal function of the prolactin promoter. In this study, the 24-bp insertion was genotyped in the fourth generation of the Silkie population selected for egg production and the relationship between the genotype of the insertion locus and performance traits was investigated. Blood samples of 69 male Silkies and 242 female Silkies were collected form the fourth generation of Silkie population selected for egg production in Livestock Research Institute of Oita Prefectural Agriculture, Forestry and Fisheries Research Center. Genomic DNA was isolated from blood samples and the fragment of the promoter of prolactin gene was amplified using PCR. The PCR products were electrophoresed on 3.0% agarose gels for genotyping. The 216 Del/Del Silkies, 26 In/Del Silkies and no In/In Silkie were found in this study. The relationship between the genotype of the insertion locus and the performance traits was analyzed by analysis of variance, however there was no relationship for growth traits and egg production. The In/Del Silkies have higher peak of the distribution of 151d-450d egg production ratio than the De/Del Silkies. Less