Regulation of proteoglycan metabolism by adiponectin in vascular smooth muscle cells

脂联素对血管平滑肌细胞蛋白多糖代谢的调节

• 批准号: 18590124
• 负责人: YAMAMOTO Chika
• 金额: $ 2.55万
• 依托单位: Hokuriku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590124/
• 关键词: vascular smooth muscle cells; proteoglycan; adiponectin; homocysteine; TNF-α; versican; decorin; atherosclerosis; 血管

Adiponectin is an adipose-specific plasma protein that exhibits antiatherogenic activities. Proteoglycans macromolecules comprising a core protein and glycosaminoglycan side chains are postulated to be the key molecules in atherosclerosis progression. In the present study, proteoglycans obtained from cultured human coronary artery smooth muscle cells after treatment with adiponectin, were characterized by biochemical techniques. The results indicated that adiponectin induces the synthesis of decorin, a small dermatan sulfate proteoglycan, in the smooth muscle cells. Furthermore, the length of dermatan sulfate chains of the decorin molecule appeared to remain unchanged, but the epimerization of glucuronic acid in the chains decreased. Although the significance of adiponectin regulation of decorin synthesis remains to be determined, the present study, for the first time, demonstrates that adiponectin regulates vascular smooth muscle cell proteoglycan synthesis.

脂联素是一种脂肪特异性血浆蛋白，具有抗动脉粥样硬化活性。包含核心蛋白和糖胺聚糖侧链的蛋白聚糖大分子被认为是动脉粥样硬化进展中的关键分子。在本研究中，通过生化技术对从培养的人冠状动脉平滑肌细胞经脂联素处理后获得的蛋白多糖进行了表征。结果表明，脂联素诱导平滑肌细胞中核心蛋白聚糖（一种小的硫酸皮肤素蛋白聚糖）的合成。此外，核心蛋白聚糖分子的硫酸皮肤素链的长度似乎保持不变，但链中葡萄糖醛酸的差向异构化减少。尽管脂联素调节核心蛋白聚糖合成的重要性仍有待确定，但本研究首次证明脂联素调节血管平滑肌细胞蛋白聚糖的合成。

项目成果

Biological Activities of Exogenous Polysaccharides via Controlling Endogenous Proteoglycan Metabolism in Vascular Endothelial Cells

外源多糖通过控制血管内皮细胞内源蛋白多糖代谢的生物学活性

• DOI: 10.1002/chin.200839270
• 发表时间: 2008
• 期刊: ChemInform
• 作者: Tomoko Sato;C. Yamamoto;Y. Fujiwara;T. Kaji
• 通讯作者: T. Kaji

アディポネクチンによる冠動脈平滑筋細胞プロテオグリカン合成の制御

脂联素对冠状动脉平滑肌细胞蛋白多糖合成的调节

• 发表时间: 2006
• 作者: Yamamoto C;Morita Y;Yamaguchi S;Hayashi T;Kaji T.;浦野 晶子;佐藤 友子;山本 千夏;藤原 泰之;Akiko Urano.;Tomoko Sato.;Yasuyuki Fujiwara;Akiko Urano;Tomoko Sato;Akiko Urano;Tomoko Sato;Tomoko Sato;浦野 晶子
• 通讯作者: 浦野 晶子

Proteoglycan as a Key Molecule in Atherosclerosis Progression: Characteristics of the Structure and Regulation of the Synthesis

蛋白多糖作为动脉粥样硬化进展的关键分子：结构特征和合成调控

• DOI: 10.1002/chin.200831254
• 发表时间: 2008
• 作者: Akiko Urano;C. Yamamoto;Y. Fujiwara;T. Kaji
• 通讯作者: T. Kaji

TNF-αによる血管平滑筋細胞プロテオグリカン合成の調節

TNF-α对血管平滑肌细胞蛋白多糖合成的调节

• 发表时间: 2007
• 作者: Yamamoto C;Morita Y;Yamaguchi S;Hayashi T;Kaji T.;浦野 晶子;佐藤 友子
• 通讯作者: 佐藤 友子

Regulation of proteoglycan synthesis by adiponectin in cultured human coronary smooth muscle cells.

在培养的人冠状动脉平滑肌细胞中脂联素对蛋白聚糖合成的调节。

• 发表时间: 2007
• 作者: Yamamoto C;Morita Y;Yamaguchi S;Hayashi T;Kaji T.;浦野 晶子;佐藤 友子;山本 千夏;藤原 泰之;Akiko Urano.;Tomoko Sato.;Yasuyuki Fujiwara;Akiko Urano;Tomoko Sato;Akiko Urano
• 通讯作者: Akiko Urano