A novelestrogen receptor in plasma membrane

近年来，GPR30被报道为一种新的雌激素受体，但其生理功能仍存在争议。为了研究GPR30在体内的作用，我们建立了GPR30的条件性基因敲除小鼠。嵌合体小鼠的受胎率在70%以上，而嵌合体小鼠的转基因未在新生小鼠中观察到。在临床患者中，我们试图建立检测GPR30基因组突变的方法。我们已经检测到了GPR30基因组的几个突变。免疫组织化学分析表明，GPR30存在于多种组织中。这些数据表明，雌激素的跨膜受体GPR30可能在多种组织中发挥作用。

Recently, GPR30 was reported to be a novel estrogen receptor; however, its physiological roles has remained controversial. To investigate the roles of GPR30 in vivo, we produced the conditional knocked out mice for GPR30. Chimera mice were bore more than 70 %, but the transgene of the chimera mice were unobserved in the newborn mice. In clinical patients, we try to develop the protocol for detection of the mutation in GPR30 genome. We have detected the a few mutant of GPR30 genome. Immunohistochemical analysis shows that GPR30 exists in various tissues. These data indicate that GPR30, a transmembrane receptor for estrogen, may play a role in various tissues.