Identification of activating factors for signal transduction released during ischemia in heart

我们从缺血心肌细胞中纯化释放的存活因子。取培养心肌细胞上清100升，硫酸铵浓缩。用柱层析法分离样品，检测心肌细胞的存活活性。用质谱法鉴定了两种具有存活活性的蛋白。这些蛋白的分子质量分别为33 kDa和41 kDa。这些蛋白诱导30kda蛋白丝氨酸磷酸化，纯化后的蛋白表现出存活活性。我们推测这些蛋白在了解心脏缺血和药物开发中发挥作用。

We purify survival factors released from cadiomyocytes exposed to ischemia. The supernatant of 100 liter was collected from cultured cardiomyocytes, and concentrated by ammonium sulfate. The sample was separated by column chromatography, and tested survival activity for cardiomyocytes. Two proteins having survival activity were identified using mass spectrometry. The molecular masses of these proteins are 33 kDa and 41 kDa, respectively. These proteins induce serine phosphorylation of 30 kDa protein, and the purified proteins show survival activity. We speculate that these proteins play a role for understanding of ischemia in heart and for development of drug discovery.