High throughput screening for G protein-coupled receptor(s) activated in response to estrogen by RNAi collection

雌激素通过核受体诱导细胞内Ca^<2+>浓度升高，但除GPR30外的膜受体尚不清楚。我们制作了用于GPCR的RNAi集合，并在RNAi集合存在的情况下检测了Ca^<2+>响应和细胞阻抗对雌激素的响应。由于雌激素反应较弱，在实验条件下未鉴定出膜受体。另一个检测系统将被要求阐明雌激素膜受体。

Estrogen induces the increase of intracellular Ca^<2+> concentrations in addition to transcriptional activity via nuclear receptor, but the membrane receptor except for GPR30 remains unknown. We have produced RNAi collection for GPCR, and examined the Ca^<2+> response and cell impedance in response to estrogen in the presence of RNAi collection. The membrane receptor did not be identified under the experimental conditions due to the weak response of estrogen. The other assay system will be requested to elucidate the estrogen membrane receptor.