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The expression and the molecular network of CSD protein in proliferating disease

CSD蛋白在增殖性疾病中的表达及分子网络

基本信息

批准号：
18590307
负责人：
UCHIUMI Takeshi
金额：
$ 2.57万
依托单位：
Kyushu University (2007)University of Occupational and Environmental Health, Japan (2006)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590307/
关键词：
Cancer Stress Gene regulation 発現抑制

项目摘要

The Y-box protein family is characterized by a highly conserved cold-shock domain that binds nucleic acids and shares homology with the prokaryotic cold-shock proteins. The eukaryotic Y-box-binding protein-1 (YB-1) is involved in the transcriptional and translational control of many biological processes, including cell proliferation. In clinical studies, the cellular level of YB-1 closely correlates with tumor growth and prognosis. To understand the role of YB-1 in vivo, especially in the developmental process, we generated YB-1 knock-out mice, which are embryonic lethal and exhibit exencephaly associated with abnormal patterns of cell proliferation within the neuroepithelium. beta-Actin expression and F-actin formation were reduced in the YB-1 null embryo and YB-1 knockout mouse embryonic fibroblasts, suggesting that the neural tube defect is caused by abnormal cell morphology and actin assembly within the neuroepithelium. Fibroblasts derived from YB-1/ embryos demonstrated reduced growth and cell density. A colony formation assay showed that YB-1/mouse embryonic fibroblasts failed to undergo morphological transformation and remained contact- inhibited in culture. These results demonstrate that YB-1 is involved in early mouse development, including neural tube closure and cell proliferation.

Y-box蛋白家族的特点是具有高度保守的冷休克结构域，该结构域与核酸结合并与原核冷休克蛋白具有同源性。真核 Y 盒结合蛋白 1 (YB-1) 参与许多生物过程的转录和翻译控制，包括细胞增殖。在临床研究中，YB-1的细胞水平与肿瘤生长和预后密切相关。为了了解 YB-1 在体内的作用，特别是在发育过程中的作用，我们培育了 YB-1 敲除小鼠，这种小鼠具有胚胎致死性，并表现出与神经上皮内细胞增殖异常模式相关的脑外畸形。 YB-1无效胚胎和YB-1敲除小鼠胚胎成纤维细胞中β-肌动蛋白表达和F-肌动蛋白形成减少，表明神经管缺陷是由神经上皮内细胞形态和肌动蛋白组装异常引起的。来自 YB-1/胚胎的成纤维细胞表现出生长和细胞密度降低。集落形成测定表明，YB-1/小鼠胚胎成纤维细胞未能经历形态转化，并且在培养物中保持接触抑制。这些结果表明 YB-1 参与小鼠早期发育，包括神经管闭合和细胞增殖。