Molecular pathologic analysis of Niban gene expression and mitochondrial alterations in thyroid cancer and oxyphilic cell tumors

甲状腺癌和嗜酸细胞肿瘤中 Niban 基因表达和线粒体改变的分子病理学分析

• 批准号: 18590347
• 负责人: FUJII Hiroaki
• 金额: $ 2.57万
• 依托单位: Juntendo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590347/
• 关键词: Niban; Cancer-related gene; Thyroid tumor; Oxyphilic cell tumor; Gene expression; Mitochondria; 甲状腺癌

Niban is a newly identified cancer-related gene, which was first reported in multi-step renal carcinogenes is model of Eker rat. Although its function is unknown, it is assumed to belong to a stress protein family. Little is know about Niban expression in human tumors. In the present study, we investigated Niban expression and its regulations in various human tissues and tumors including thyroid, oxyphilic and other tumors. Up-regulation of Niban was frequently detected in thyroid tumors including papillary and oxyphilic tumors, head & neck squamous cell carcinomas and head & neck squamous dysplasias. Immunohistochemical results correlated with RT-PCR results and Western blot analysis. No mutations of Niban were detected in the exons and in the promoter. Methylation of Niban promoter CpG island was not detected in tumors studied. Niban expression was not correlated with BRAF and GRIM mutations, which were frequently reported in poapillary and oxyphilic tumors. In summary, up-regulation of Niban may play an important role in the carcinogenic process of some tumors. Although relationship of Niban expression and altered mitochondrial functions were speculated, no direct evidence for such relationship was found. Because we did not detect any mutations or methylations in Niban gene, it is unlikely that Niban belongs to classical groups of oncogenes and/or tumor suppressor genes.

Niban是一种新确定的癌症相关基因，在多步肾癌中首次报道了Eker大鼠的模型。尽管其功能尚不清楚，但假定它属于应激蛋白家族。对人类肿瘤中尼巴的表达知之甚少。在本研究中，我们研究了Niban的表达及其在包括甲状腺，氧气和其他肿瘤在内的各种人体组织和肿瘤中的调节。在甲状腺肿瘤，包括乳头状肿瘤，头颈部鳞状细胞癌以及头部和颈部鳞状发育不良的甲状腺肿瘤中经常检测到尼巴的上调。免疫组织化学结果与RT-PCR结果和蛋白质印迹分析有关。在外显子和启动子中未检测到尼巴的突变。在研究的肿瘤中未检测到Niban启动子CpG岛的甲基化。 Niban的表达与BRAF和Grim突变无关，这些突变经常在毛皮和氧气肿瘤中报道。总而言之，尼巴的上调可能在某些肿瘤的致癌过程中起重要作用。尽管猜测了尼巴族表达和线粒体功能改变的关系，但未直接证明这种关系。因为我们没有检测到尼巴尼基因中的任何突变或甲基化，所以尼巴恩不太可能属于癌基因和/或肿瘤抑制基因的经典组。

项目成果

Expression analysis of Niban, a new cancer-related molecular marker in thyroid tumors

甲状腺肿瘤中新的癌症相关分子标志物Niban的表达分析

• 发表时间: 2006
• 作者: Matsumoto;F.;Fujii;H.;et. al.
• 通讯作者: et. al.

Mesothelin expression and its methylation in human gynecological tumors

人妇科肿瘤中间皮素的表达及其甲基化

• 发表时间: 2008
• 作者: Gulanbar;U.;Fujii;H.;et. al.
• 通讯作者: et. al.

Expression analysis of Niban, a new cancer-related gene in various human tumors

新型癌症相关基因Niban在多种人类肿瘤中的表达分析

• 发表时间: 2007
• 作者: Fujii;H.;et. al.
• 通讯作者: et. al.

Expression analysis of Mesothelin, a mesothelioma related gene in combined hepatocelluar and chlangiocarcinoma, and intrahepatic cholangiocarcinoma

间皮瘤相关基因间皮素在肝细胞胆管癌及肝内胆管癌中的表达分析

• 发表时间: 2007
• 作者: Nomura;R.;Fujii;H.;et. al.
• 通讯作者: et. al.

Expression of Niban, a novel cancer-related gene in head & neck squamous cell carcinoma and dyplasia

头部新型癌症相关基因Niban的表达

• 发表时间: 2008
• 作者: Ito;S.;Fujii;H.;et. al.
• 通讯作者: et. al.