Molecular pathologic analysis of Niban gene expression and mitochondrial alterations in thyroid cancer and oxyphilic cell tumors

甲状腺癌和嗜酸细胞肿瘤中 Niban 基因表达和线粒体改变的分子病理学分析

• 批准号: 18590347
• 负责人: FUJII Hiroaki
• 金额: $ 2.57万
• 依托单位: Juntendo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590347/
• 关键词: Niban; Cancer-related gene; Thyroid tumor; Oxyphilic cell tumor; Gene expression; Mitochondria; 甲状腺癌

Niban is a newly identified cancer-related gene, which was first reported in multi-step renal carcinogenes is model of Eker rat. Although its function is unknown, it is assumed to belong to a stress protein family. Little is know about Niban expression in human tumors. In the present study, we investigated Niban expression and its regulations in various human tissues and tumors including thyroid, oxyphilic and other tumors. Up-regulation of Niban was frequently detected in thyroid tumors including papillary and oxyphilic tumors, head & neck squamous cell carcinomas and head & neck squamous dysplasias. Immunohistochemical results correlated with RT-PCR results and Western blot analysis. No mutations of Niban were detected in the exons and in the promoter. Methylation of Niban promoter CpG island was not detected in tumors studied. Niban expression was not correlated with BRAF and GRIM mutations, which were frequently reported in poapillary and oxyphilic tumors. In summary, up-regulation of Niban may play an important role in the carcinogenic process of some tumors. Although relationship of Niban expression and altered mitochondrial functions were speculated, no direct evidence for such relationship was found. Because we did not detect any mutations or methylations in Niban gene, it is unlikely that Niban belongs to classical groups of oncogenes and/or tumor suppressor genes.

尼班是一个新发现的肿瘤相关基因，在Eker大鼠多步肾癌模型中首次报道。虽然其功能尚不清楚，但被认为属于应激蛋白家族。关于Niban在人类肿瘤中的表达知之甚少。在本研究中，我们研究了Niban在各种人体组织和肿瘤（包括甲状腺、嗜酸细胞和其他肿瘤）中的表达及其调控。尼班的上调在甲状腺肿瘤中经常检测到，包括乳头状和嗜酸性肿瘤、头颈部鳞状细胞癌和头颈部鳞状发育不良。免疫组化结果与RT-PCR结果和Western blot分析相关。在外显子和启动子中未检测到尼班突变。Niban启动子CpG岛甲基化在肿瘤中未检测到。Niban表达与BRAF和GRIM突变无关，这些突变在乳头状和嗜酸性肿瘤中经常报告。综上所述，Niban的上调可能在某些肿瘤的致癌过程中发挥重要作用。虽然推测了Niban的表达与线粒体功能改变的关系，但没有发现这种关系的直接证据，因为我们没有检测到Niban基因的任何突变或甲基化，Niban不太可能属于经典的癌基因和/或肿瘤抑制基因。

项目成果

Mesothelin expression and its methylation in human gynecological tumors

人妇科肿瘤中间皮素的表达及其甲基化

• 发表时间: 2008
• 作者: Gulanbar;U.;Fujii;H.;et. al.
• 通讯作者: et. al.

Expression analysis of Niban, a new cancer-related gene in various human tumors

新型癌症相关基因Niban在多种人类肿瘤中的表达分析

• 发表时间: 2007
• 作者: Fujii;H.;et. al.
• 通讯作者: et. al.

Expression analysis of Niban, a new cancer-related molecular marker in thyroid tumors

甲状腺肿瘤中新的癌症相关分子标志物Niban的表达分析

• 发表时间: 2006
• 作者: Matsumoto;F.;Fujii;H.;et. al.
• 通讯作者: et. al.

Birt–Hogg–Dubé gene mutations in human endometrial carcinomas with microsatellite instability

• DOI: 10.1002/path.1992
• 发表时间: 2006-07
• 期刊: The Journal of Pathology
• 作者: H. Fujii;W. Jiang;Toshiharu Matsumoto;K. Miyai;K. Sashara;N. Ohtsuji;O. Hino

Allelic loss analysis of early-stage flat -type color-ectal tumors.

早期扁平型结直肠肿瘤的等位基因丢失分析。

• 发表时间: 2006
• 期刊: Ann Pathol 17
• 作者: Orita H;Sakamoto T;Kamano T;Tsurumaru M;Fujii H
• 通讯作者: Fujii H