Roles of Monocytes/Macrophage for Acquired Immunity in Helicobacter pylori Infection

单核细胞/巨噬细胞在幽门螺杆菌感染中获得性免疫的作用

• 批准号: 18590425
• 负责人: YOKOTA Kenji
• 金额: $ 2.57万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590425/
• 关键词: Helicobacter pylori; macrophage; DEC205; MAPK; TLR; HSP60; 動脈硬化

We have been studying functions of macrophage in Hefrcobacter pylori inffection. Because we think that acquired immunity may play important roles in the diseases caused by H. pylori infection. To investigate immunity of H.pylori infection, we have shown the H.pylori- HSP60 (heat shock protein 60 kDa) activates macrophage and it is useful antigen.We have shown some results supporting by this Grant.1) HSP60 stimulated and activated a macrophage though TLR2 and TLR4. IL8 was produced from the macrophage depending on MAPK and NE-〓B activation.2) Arteriosclerosis in Apo-E KO mouse was progressed by H. pylori infection. The pathology was associated with immunity to HSP60 and T helper 1.3) Patients with arteriosclerosis possessed antibody to peptides from H. pylori-HSP60. The peptides might be available for diagnosis of arteriosclerosis.4) DNA chip study indicated that DEC205 is an important receptor for macrophage activation in patients with gastritis caused by H pylori infection. We are now studying function of DEC205 on macrophage using NOMO-1 cell differenciated by PMA.

我们一直在研究巨噬细胞在幽门螺杆菌感染中的作用。因为我们认为获得性免疫可能在幽门螺杆菌感染引起的疾病中起重要作用。为了研究幽门螺杆菌感染的免疫功能，我们发现HSP60(热休克蛋白60 kDa)能激活巨噬细胞，是一种有用的抗原。IL 8的产生依赖于MAPK和NE-〓B的激活。2)幽门螺杆菌感染导致ApoE KO小鼠动脉硬化。其病理机制与对HSP60和辅助性T细胞的免疫反应有关。1)动脉硬化症患者存在抗HSP60多肽抗体。4)DNA芯片研究表明，DEC205是幽门螺杆菌感染所致胃炎患者巨噬细胞活化的重要受体。我们正在用PMA分化的NOMO-1细胞研究DEC205对巨噬细胞的作用。

项目成果

Expression of endocytoric receptor DEC205 in mdcrophagei Helicobacter pylori infection

噬菌体幽门螺杆菌感染中内吞受体DEC205的表达

• 作者: Yokita;K.;Zhao;Y.;Fiijirnoto;K.;Ayada;K.;Hirai;K.;Ogama;K
• 通讯作者: K

Conversion of Flavodoxin from holoenayme to apoprotein during growth phase changes in Helicobacter pylori

幽门螺杆菌生长期变化过程中黄素氧还蛋白从全藻素转化为脱辅基蛋白

• 发表时间: 2007
• 期刊: J Bacteriol 189
• 作者: Shimomura H;Hayashi S;Yokota K;Oguma K;Hirai Y.
• 通讯作者: Hirai Y.

動脈硬化の検査方法

动脉硬化试验方法

• 发表时间: 2013

Antibodies against heat shock protein 60 derived from Helicobacter pylori: diagnostic implications hl cardiovascular disease.

源自幽门螺杆菌的热休克蛋白 60 抗体：心血管疾病的诊断意义。

• 发表时间: 2007
• 期刊: J Autoimmun 29
• 作者: Okada T;Ayada K;Usui S;Yokota K;Cui J;Kawahara Y;Inaba T;Hirohata S;Mizuno M;Yamamoto D;Kusachi S;Matsuura E;Oguma K.
• 通讯作者: Oguma K.

Helicobacter pylori heat-shock protein 60 induces interleukin-8 via a Toll-like receptor (TLR)2 and mitogen-activated protein (MAP) kinase pathway in human monocytes.

• DOI: 10.1099/jmm.0.46882-0
• 发表时间: 2007-02
• 期刊: Journal of medical microbiology
• 影响因子: 3
• 作者: Yingxin Zhao;K. Yokota;K. Ayada;Yumiko Yamamoto;Tomayuki Okada;Lianhua Shen;K. Oguma