Roles of Monocytes/Macrophage for Acquired Immunity in Helicobacter pylori Infection

我们一直在研究巨噬细胞在幽门螺杆菌感染中的作用。我们认为获得性免疫可能在H.幽门感染为了研究幽门螺杆菌感染的免疫性，我们已经证明H.pylori-HSP60（热休克蛋白60 kDa）激活巨噬细胞，它是有用的抗原。我们已经证明了本资助的一些结果。1）HSP60通过TLR 2和TLR 4刺激和激活巨噬细胞。2）Apo-E基因敲除小鼠动脉粥样硬化是由H.幽门感染动脉硬化患者血清中存在抗H.幽门螺杆菌-HSP60。4）DNA芯片研究表明DEC 205是幽门螺杆菌感染所致胃炎患者巨噬细胞活化的重要受体。我们正在用PMA诱导的NOMO-1细胞研究DEC 205对巨噬细胞的作用。

We have been studying functions of macrophage in Hefrcobacter pylori inffection. Because we think that acquired immunity may play important roles in the diseases caused by H. pylori infection. To investigate immunity of H.pylori infection, we have shown the H.pylori- HSP60 (heat shock protein 60 kDa) activates macrophage and it is useful antigen.We have shown some results supporting by this Grant.1) HSP60 stimulated and activated a macrophage though TLR2 and TLR4. IL8 was produced from the macrophage depending on MAPK and NE-〓B activation.2) Arteriosclerosis in Apo-E KO mouse was progressed by H. pylori infection. The pathology was associated with immunity to HSP60 and T helper 1.3) Patients with arteriosclerosis possessed antibody to peptides from H. pylori-HSP60. The peptides might be available for diagnosis of arteriosclerosis.4) DNA chip study indicated that DEC205 is an important receptor for macrophage activation in patients with gastritis caused by H pylori infection. We are now studying function of DEC205 on macrophage using NOMO-1 cell differenciated by PMA.