The Study of Cell Cycle Regulation and Epithelial-Mesenchymal Transition

细胞周期调控与上皮间质转化的研究

基本信息

  • 批准号:
    18590844
  • 负责人:
  • 金额:
    $ 2.17万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2006
  • 资助国家:
    日本
  • 起止时间:
    2006 至 2007
  • 项目状态:
    已结题

项目摘要

Background: Epithelial mesenchymal transition (EMT) is known as the process by which differentiated epithelial cells undergo phenotypic transition to mesenchymal cells. Possibly, this process may occur in certain airway fibrotic diseases involving airway remodeling.Objectives: To clarify whether airway epithelial cells can differentiate mesenchymal cells through EMT.Methods: Mouse tracheal epithelial cells (mTEC) from BALB/c mouse were isolated, and cultured in an air-liquid interface (ALI) system in the presence or absence of TGF-p I. The expression of a-smooth muscle actin (a-SMA), vimentin, zonula occludens-I (Zo-I) and occludin was examined by immunofluorescence staining and westem blotting. Production of matrix metalloproteinase (MMP)-9 in culture medium was measured by enzyme linked immunosorbent assay.Results: Immunofluorescent staining revealed that TOF-p I treatment for 14 days induced the expression of mesenchymal markers, a-SMA and vimentin, and decreased the expression.of epithelial markers, Zo-1 and occluding in mTEC. Additionally, a-SMA and Zo-1 were colocalized within the single cells treated with TGF-(1l. In western blotting analysis, TGF-p1 treatment significantly enhanced the expression of a-SMA and vimentin, while it significantly reduced the expression of Zo-1 and occluding over time. Concentrations of MMP-9 in the culture medium were significantly higher in mTEC treated with TOF-β1 than those non-treated.Conclusions: These data suggest that EMT was induced by TGF-βl in the primary culture of mTEC with the ALI system, leading to the possibility that airway epithelial cells participates in airway remodeling through EMT.
背景资料:上皮间质转化(EMT)是指上皮细胞向间质细胞转化的过程。方法:分离BALB/c小鼠气管上皮细胞(mTEC),在有或无TGF-β I的气液界面(air-liquid interface,ALI)培养体系中培养,观察MTEC的分化情况。免疫荧光染色和Westem印迹法检测α-平滑肌肌动蛋白(α-SMA)、波形蛋白(vimentin)、闭锁小带-I(Zo-I)和闭锁蛋白(occludin)的表达。结果:TOF-p I处理mTEC 14 d后,mTEC中基质金属蛋白酶-9(MMP-9)的表达明显增加,α-SMA和vimentin的表达明显增加,而上皮标志物ZO-1和occluding的表达明显减少。此外,α-SMA和Zo-1共定位在用TGF-β 1处理的单细胞内。在蛋白质印迹分析中,TGF-β 1处理显著增强了α-SMA和波形蛋白的表达,而随着时间的推移,它显著降低了Zo-1和occluding的表达。结论:TGF-β 1可诱导mTEC原代培养过程中发生EMT,提示气道上皮细胞可能通过EMT参与气道重塑。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Correlation between peripheral blood T-cell profiles and airway inflammation in atopic asthma
特应性哮喘患者外周血 T 细胞谱与气道炎症的相关性
  • DOI:
  • 发表时间:
    2006
  • 期刊:
  • 影响因子:
    0
  • 作者:
    shirai;T.;Suda;T.;et. al.
  • 通讯作者:
    et. al.
Japan idiopathic pulmonary fibrosis study group : Dietary fat and meat intake and idiopathic pulmonary fibrosis : a case-control study in Japan
日本特发性肺纤维化研究组:膳食脂肪和肉类摄入与特发性肺纤维化:日本的病例对照研究
  • DOI:
  • 发表时间:
    2006
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Sasaki;S.;Yokoyama;T.;Chida;K.;Azuma;A.;Suda;T.;Kudoh;S.;Sakamoto;N.;Okamoto;K.;Kobashi;G.;Washfo;M.;Inaba;Y.;Tanaka;H
  • 通讯作者:
    H
Lung dendritic cells have a potent capability to induce production of immunoglobulin A
Immunization with dendritic cells retrovirally transduced with mycobacterial antigen 85A gene elicits the specific cellular immunity including cytotoxic T-lymphocyte activity specific to an epitope on antigen 85A
用分枝杆菌抗原 85A 基因逆转录病毒转导的树突状细胞进行免疫可引发特异性细胞免疫,包括对抗原 85A 上的表位具有特异性的细胞毒性 T 淋巴细胞活性
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Ansari AA;Mayne AE;Onlamoon N;Pattanapanyasat K;Mori K;Villinger F.;大友一雄;大友一雄;大友 一雄;五島 敏芳;神立 孝一;岡田全司;岡田全司;岡田全司;岡田全司;岡田全司;岡田全司;岡田全司;岡田全司;岡田全司;岡田全司;岡田全司;岡田全司;岡田全司;岡田全司;岡田全司;岡田全司;岡田全司;岡田全司;岡田全司;岡田全司;岡田全司;岡田全司
  • 通讯作者:
    岡田全司
A validation and potential modification of the pneumonia severity index in elderly patients with community-acquired pneumonia
  • DOI:
    10.1111/j.1532-5415.2006.00825.x
  • 发表时间:
    2006-08-01
  • 期刊:
  • 影响因子:
    6.3
  • 作者:
    Naito, Tateaki;Suda, Takafumi;Nakamura, Hirotoshi
  • 通讯作者:
    Nakamura, Hirotoshi
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CHIDA Kingo其他文献

CHIDA Kingo的其他文献

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{{ truncateString('CHIDA Kingo', 18)}}的其他基金

The study on administration route of T-cell independent vaccinefrom the point view of bronchus-associated lymphoid tissue
从支气管相关淋巴组织角度研究T细胞非依赖性疫苗给药途径
  • 批准号:
    22590855
  • 财政年份:
    2010
  • 资助金额:
    $ 2.17万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Immunohistochemical study of cell cycle regulators in idiopathic pulmonary fibrosis
特发性肺纤维化细胞周期调节因子的免疫组织化学研究
  • 批准号:
    15590802
  • 财政年份:
    2003
  • 资助金额:
    $ 2.17万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The significance of expression of CD1 family by dendritic cells in human sarcoid lesions
人结节病灶中树突状细胞表达CD1家族的意义
  • 批准号:
    13670594
  • 财政年份:
    2001
  • 资助金额:
    $ 2.17万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
A Monoclonal Antibody Directed Against Activation Antigen on Alveolar Macrophages.
针对肺泡巨噬细胞激活抗原的单克隆抗体。
  • 批准号:
    01570427
  • 财政年份:
    1989
  • 资助金额:
    $ 2.17万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

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Immune evasion mechanisms and pathogenesis-related factors of mycoplasma in bovine tracheal epithelial cells.
牛气管上皮细胞支原体免疫逃避机制及发病相关因素
  • 批准号:
    23K05574
  • 财政年份:
    2023
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Novel transcription factor to regulate differentiation of tracheal epithelial cells.
调节气管上皮细胞分化的新型转录因子。
  • 批准号:
    09672239
  • 财政年份:
    1997
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DIFFERENTIATION AND DIFFERENTIATIVE FUNCTIONS OF TRACHEAL EPITHELIAL CELLS
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  • 批准号:
    4693202
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    $ 2.17万
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