The significance of expression of CD1 family by dendritic cells in human sarcoid lesions

人结节病灶中树突状细胞表达CD1家族的意义

• 批准号: 13670594
• 负责人: CHIDA Kingo
• 金额: $ 1.41万
• 依托单位: Hamamatsu University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670594/
• 关键词: Sarcoidosis; CD1; lipid antigens; granuloma formation; 肉芽種形成; CD1分子; 肉芽腫; 樹状細胞

To study whether or not lipid antigens are one of causative agents of sarcoidosis, we investigated the expression of the CD1 family, lipid antigen-presenting molecules, by dendritic cells in human sarcoid lesions.The following antibodies were used for immunohistochemical studies : anti-CD1a(O10), anti-CD1b(4A7.6.5), anti-CD1c(L161), and anti-CD83(IM2069). Biopsied specimens were obtained from 8 patients with sarcoidosis : skin lesion from 1 patient, lymph nodes from 2 patients, pulmonary lesion from 1 patient, and muscle lesions from 4 patients. CD1 proteins were positively expressed by dendritic cells in sarcoid lesions from 4 patients. These positive cells were distributed inside granulomas as well as around granulomas. There were no relations between the cases with CD1 positive cells and their disease activities. In addition, the experimental models on B57BL/6 mice showed granuloma formation that were compatible to sarcoid lesions by the administration of trechalose-6, 6'-dimycolate.These results indicate that the CD1 system may play an important role in the development of sarcoid lesions.

为了研究脂类抗原是否是结节病的致病因素之一，我们研究了CD1家族--脂类抗原递呈分子--在人类结节病变树突状细胞中的表达。活检标本取自8例结节病患者，其中皮肤病变1例，淋巴结病变2例，肺部病变1例，肌肉病变4例。4例结节样皮损中树突状细胞CD1蛋白表达阳性。阳性细胞分布于肉芽肿内及肉芽肿周围。CD1阳性细胞病例与疾病活动无关。此外，在B57BL/6小鼠上的实验模型显示，给予6，6‘-二羟甲基海藻糖可形成与结节样病变相容的肉芽肿，这些结果表明CD1系统在结节样病变的发生发展中可能起重要作用。

项目成果

Nakano Y. et al.: "Efficacy of a consensus protocol therapy in adults with acute, severe asthma"Ann Allergy, Asthma, Immunol. 90. 331-337 (2003)

Nakano Y. 等人：“共识方案治疗成人急性、重度哮喘的疗效”Ann Allergy、Asthma、Immunol。

Inui N, Kitagawa K, Miwa S, Hattori T, Chida K, Nakamura H, Kitagawa M: "High expression of Cks1 in human non-small cell lung carcinomas"Biochem Biophys Research Commun. 303. 978-984 (2003)

Inui N、Kitakawa K、Miwa S、Hattori T、Chida K、Nakamura H、Kitakawa M：“人类非小细胞肺癌中 Cks1 的高表达”Biochem Biophys Research Commun。

Yamada T, Uchiyama H, Nagata T, Uchijima M, Suda T, Chida K, Nakamura H, Koide Y: "Protective cytotoxic T lymphocyte responses induced by DNA immunization against immunodominant and subdominant epitopes of Listeria monocytogenesis are noncompetitive"Infec

Yamada T、Uchiyama H、Nagata T、Uchijima M、Suda T、Chida K、Nakamura H、Koide Y：“针对单核细胞生成李斯特菌的免疫显性和次显性表位的 DNA 免疫诱导的保护性细胞毒性 T 淋巴细胞反应是非竞争性的”Infec

Inui N, Suda T, Chida K, Nakamura H: "Th1/Th2 and Tc1/Tc2 profiles in peripheral blood and bronchoal veolar lavage fluid cells in pulmonary sarcoidosis"J Allergy Clin Immunol. 107(2). 924-925 (2001)

Inui N、Suda T、Chida K、Nakamura H：“肺结节病外周血和支气管肺泡灌洗液细胞中的 Th1/Th2 和 Tc1/Tc2 特征”J Allergy Clin Immunol。

Suda T, Chida K, Todate A, Ide K, Asada K, Nakamura Y, Suzuki K, Kuwata H, Nakamura H: "Oncostatin m production by human dendritic cells in response to bacterial products"Cytokine. 17(6). 335-340 (2002)

Suda T、Chida K、Todate A、Ide K、Asada K、Nakamura Y、Suzuki K、Kuwata H、Nakamura H：“人树突状细胞响应细菌产物产生制瘤素 m”细胞因子。