Real-time analysis of peritoneal function from Nitric Oxide (NO) and Reactive Oxygen Species (ROS)

实时分析一氧化氮 (NO) 和活性氧 (ROS) 的腹膜功能

• 批准号: 18590915
• 负责人: SASAKI Tamaki
• 金额: $ 2.44万
• 依托单位: Kawasaki Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590915/
• 关键词: CAPD; Nitric oxide(NO); NO sensor; Oxidative stress; Tetrahydrobionterin(BH_4); ADMA; tetrahydrobiopterin(BH4); Asymmetric

Nitric oxide (NO) plays a key role in the regulation of vascular tone and controls both local and systemic hemodynamics. Also, dysfunction of the peritoneal membrane has been suggested to be related to abnormal production and/or metabolism of endogenous NO. Tetrahydrobiopterin (BH_4) is a key cofactor to maintain the enzymatic activity of nitric oxide synthase (NOS). A decrease in BH_4 level may cause endothelial NOS uncoupling, resulting in reduced NO synthesis and enhanced superoxide release from NOS. The aim of the present study was thus to measure NO, BH_4 and BH_2 (an oxidized form of BH_4) concentrations in the dialysate effluent of CAPD patients. Eighteen patients with chronic renal failure undergoing peritoneal dialysis at our hospital participated in this study. We directly measured the concentration of NO in the CAPD dialysate using an NO sensor (amiNO-700, Innovative Instruments, Inc., USA). Both BH_4 and BH_2 concentrations were measured using HPLC. NO concentration was significantly higher in the dialysate after 4 hrs CAPD than in the dialysate immediately after an exchange of CAPD solution (8.3±3.2 nM vs. 3.3±0.9 nM; p<0.05). BH_4 concentration was also higher after a 4-hr CAPD than immediately after solution exchange (4.10±4.18 ng/ml vs. 0.29±0.85 ng/ml; p<0.05). BH2 concentration also showed the same trend (0.18±0.24 ng/ml vs. 0.07±0.10 ng/ml; p<0.05). In the CAPD therapy, both NO and BH_4 concentrations increased in the course of time. These results suggest that the bioavailability of peritoneum-derived NO is increased by CAPD therapy due to the increased BH_4 availability.

一氧化氮 (NO) 在调节血管张力并控制局部和全身血流动力学方面发挥着关键作用。此外，腹膜功能障碍也被认为与内源性 NO 的异常产生和/或代谢有关。四氢生物蝶呤 (BH_4) 是维持一氧化氮合酶 (NOS) 酶活性的关键辅助因子。 BH_4水平的降低可能导致内皮NOS解偶联，导致NO合成减少和NOS释放超氧化物增加。因此，本研究的目的是测量 CAPD 患者透析液流出物中的 NO、BH_4 和 BH_2（BH_4 的氧化形式）浓度。 18 名在我院接受腹膜透析的慢性肾功能衰竭患者参与了本研究。我们使用 NO 传感器（amiNO-700，Innovative Instruments，Inc.，美国）直接测量 CAPD 透析液中的 NO 浓度。使用 HPLC 测量 BH_4 和 BH_2 浓度。 4 小时 CAPD 后透析液中的 NO 浓度显着高于更换 CAPD 溶液后立即透析液中的 NO 浓度（8.3±3.2 nM vs. 3.3±0.9 nM；p<0.05）。 4小时CAPD后的BH_4浓度也比溶液交换后立即更高(4.10±4.18ng/ml vs.0.29±0.85ng/ml；p<0.05)。 BH2 浓度也显示出相同的趋势（0.18±0.24 ng/ml 对比 0.07±0.10 ng/ml；p<0.05）。在CAPD治疗中，NO和BH_4浓度随着时间的推移而增加。这些结果表明，由于 BH_4 可用性增加，CAPD 治疗增加了腹膜来源的 NO 的生物利用度。

项目成果

腹膜透析液中NOと関連物質の動態評価

腹膜透析液中NO及相关物质的动力学评价

• 发表时间: 2007
• 期刊: 信学技報IEICE Technical Report Vol.157,No.154
• 作者: 滝沢夏生;山本健一郎;松田雅人;薬師寺大二;福田誠;宮坂武寛;酒井清孝;高山 綾
• 通讯作者: 高山 綾

Direct Measurement of Nitric Oxide and Tetrahydrobiopterin Levels in Peritoneal Dialysate

直接测量腹膜透析液中一氧化氮和四氢生物蝶呤水平

• 发表时间: 2007
• 期刊: Perit Dial Int 27
• 作者: TAKAYAMA A.;et. al.
• 通讯作者: et. al.

Direct measurement of nitric oxide concentration in CAPD dialysate

直接测量 CAPD 透析液中的一氧化氮浓度

• 发表时间: 2008
• 期刊: Perit. Dial. Int. 29(1)
• 作者: S Mochizuki;A Takayama;T Sasaki;H Horike;N Kashihara;Y Ogasawara;F Kajiya;T Kitawaki;H Oka
• 通讯作者: H Oka

Direct Measurement of Nitric Oxide Levels in Peritoneal Dialysate

直接测量腹膜透析液中的一氧化氮水平

• 发表时间: 2008
• 期刊: PD Int
• 作者: Mochizuki Seiichi;et. al.
• 通讯作者: et. al.

Direct measurement of effluent nitric oxide levels in CAPD patients

直接测量 CAPD 患者流出物一氧化氮水平

• 发表时间: 2006
• 期刊: Perit Dial Int 26
• 作者: TAKAYAMA A.;et. al.;SASAKI T
• 通讯作者: SASAKI T