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Immunohistochemical analysis of TGF-βand Smad in glomerular damage

TGF-β和Smad在肾小球损伤中的免疫组织化学分析

基本信息

批准号：
11671062
负责人：
SASAKI Tamaki
金额：
$ 0.7万
依托单位：
KAWASAKI MEDICAL SCHOOL
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671062/
关键词：
TGF-β Smad glomerular epithelial cell mesotehlail cell Sclerosing peritonitis Smad

项目摘要

1) Immunohistochemical study of the expression of TGF-β and Smad in glomerular epithelial cells of various glomerular injury models. A mesangial proliferative GN model induced by an anti-Thy 1 antibody, a glomerular epithelial cell injury model induced by Puromycin Aminonucleoside in Wister rats, and a crescentic formation model induced by an anti- GBM antibody in WKY rats were made, and kidneys of the rats were removed and observed. In these models, TGF-β1 remained unchanged, but the expression of β2 and β3 increased in the cytoplasm of glomerular epithelial cells. All the Smads were observed in the cytoplasm of these cells.2) Immunohistochemical study of the expression of TGF- β and Smad in peritoneal sclerotic lesions Sclerosing peritonitis is a complication associated with a long-term CAPD.Localization of TGF-βwas examined in peritoneal biopsy tissues of sclerotic peritonitis using an immunohistochemical method. While TGF-β1, β2 and β3 were stained slightly in some normal peritoneal mesothelial cells, their receptors RI and R2 were stained in all normal peritoneal mesothelial cells. On the other hand, increased staining of TGF-β1, β2 andβ3 in peritoneal mesothelial cells was observed in cases of sclerosing peritonitis. With the expression of β2 and β3 being particularly strong. In the Smad family, which is the intracellular signaling system of TGF-β, Smad 4 was found in the mesothelial cells of normal peritoneum. There were also some mesothelial cells in which expression of the inhibiting type of Smad 6 and Smad 7 could be confirmed. Conclusion : Suppression of the Smad family (the intracellular signaling system of TGF-β) may be effective for suppressing the action of TGF-β and thereby preventing glomerular and peritoneal lesions.

1)不同肾小球损伤模型肾小球上皮细胞转化生长因子-β和Smad表达的免疫组织化学研究。建立抗Thy-1抗体诱导的系膜增生性肾炎模型、嘌呤霉素氨基核苷诱导的Wistar大鼠肾小球上皮细胞损伤模型、抗GBM抗体诱导的WKY大鼠新月体形成模型，取大鼠肾脏进行观察。在这些模型中，转化生长因子-β-1没有变化，但β-2和β-3在肾小球上皮细胞胞浆中的表达增加。2)腹膜硬化性病变中转化生长因子-β和Smad表达的免疫组织化学研究硬化性腹膜炎是一种与长期CAPD相关的并发症。转化生长因子-β-1、β-2和β-3在部分正常腹膜间皮细胞呈弱阳性表达，其受体RI和R2在所有正常腹膜间皮细胞均呈阳性表达。硬化性腹膜炎患者腹膜间皮细胞转化生长因子-β-1、β-2和β-3表达增强。其中β-2和β-3的表达尤为强烈。在转化生长因子-β的细胞内信号转导系统Smad4家族中，Smad4存在于正常腹膜间皮细胞中。间皮细胞中也可见抑制型Smad6和Smad7的表达。结论：抑制Smad家族(转化生长因子-β的细胞内信号系统)可能有效地抑制转化生长因子-β的作用，从而预防肾小球和腹膜病变。