Leptomeningeal Collateral Growth alter Chronic Mild Cerebral Ischemia - Effect of Gianulocyte-macrophage Colony Stimulating Factor -

软脑膜侧枝生长改变慢性轻度脑缺血 - 巨噬细胞集落刺激因子的作用 -

• 批准号: 18590937
• 负责人: KITAGAWA Kazuo
• 金额: $ 2.43万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590937/
• 关键词: cerebral ischemia; collateral circulation; GM-CSF; chronic ischemia; arteriogenesis; 脳軟膜動脈吻合; 顆粒球マクロファージコロニー刺激因子; 脳梗塞; 脳血流; 慢性低灌流; 脳血流自動調節

Granulocyte-macrophage colony stimulating factor (GM-CSF) has been reported to accelerate collateral growth (arteriogenesis) at the circle of Willis in rat brain. However, the effect of GM-CSF on leptomeninegal collateral growth has not been established. We examined the effect of unilateral common carotid artery (CCA) occlusion and GM-CSF treatment on leptomeninegal collateral growth in mice.Adult mice were subjected to unilateral CCA occlusion or sham surgery followed by an alternate-day regimen of GM-CSF (20 μg/kg) or saline injection. On day 7, latex perfusion was performed in 1 set of mice to visualize the leptomeningeal vessels, and the number of Mac-2 (+) monocyte/macrophages on the dorsal surface of the brain was counted. In another set of mice, on day 7, permanent ipsilateral middle cerebral cerebral artery (MCA) occlusion was performed, and infarct volume was measured.Leptomeningeal collateral growth was observed after CCA occlusion, and that was enhanced by GM-CSF treatment. An increase in the number of Mac-2(+) cells on the surface of the brain occurred after CCA occlusion and was enhanced by GM-CSF treatment. Seven days after CCA occlusion, GM-CSF treatment decreased the infarct size attributable to subsequent MCA occlusion.After CCA occlusion, GM-CSF treatment enhanced leptomeningeal collateral growth and decreased the infarct size after MCA occlusion in mice.

粒细胞-巨噬细胞集落刺激因子（GM-CSF）已被报道可加速大鼠脑Willis环的侧支生长（动脉生成）。然而，GM-CSF对柔脑膜侧支生长的影响尚未确定。我们研究了单侧颈总动脉（CCA）闭塞和GM-CSF治疗对小鼠软脑膜侧支生长的影响。成年小鼠接受单侧CCA闭塞或假手术，随后隔日注射GM-CSF（20 μg/kg）或生理盐水。在第7天，在1组小鼠中进行乳胶灌注以使软脑膜血管可视化，并对脑背表面上的Mac-2（+）单核细胞/巨噬细胞的数量进行计数。在另一组小鼠中，在第7天，进行永久性同侧大脑中动脉（MCA）闭塞，测量梗死体积，观察CCA闭塞后软脑膜侧支生长，并且GM-CSF治疗增强。CCA闭塞后，大脑表面Mac-2（+）细胞数量增加，GM-CSF治疗后增加。CCA闭塞后7天，GM-CSF治疗可减少随后MCA闭塞引起的梗死面积，CCA闭塞后，GM-CSF治疗可促进小鼠软脑膜侧支生长，并减少MCA闭塞后的梗死面积。

项目成果

Granulocyte-macrophage colony stimulating factor treatment after common carotid artery occlusion enhances leptomeningeal collateral growth and reduces infarct size after focal cerebral ischemia

颈总动脉闭塞后粒细胞-巨噬细胞集落刺激因子治疗可增强局灶性脑缺血后软脑膜侧枝生长并减少梗塞面积

• 发表时间: 2008
• 作者: K.;Todo;K.;Kitagawa;T.;Sasaki;E.;Omura-Matsuoka;Y.;Terasaki;N.;Oyama;Y.;Yagita;M.;Hori
• 通讯作者: Hori

CREB and CRE-mediated gene expression in the ischemic brain.

CREB ​​和 CRE 介导的缺血脑中的基因表达。

• 发表时间: 2007
• 期刊: FEBS Journal, 274
• 作者: Todo K;Kitagawa K;Sasaki T;Omura-Matsuoka E;Terasaki Y;Oyama N;Yagita Y;Hori M;Kitagawa K.
• 通讯作者: Kitagawa K.

Granulocyte-Macrophage Colony-Stimulating Factor Enhances Leptomeningeal Collateral Growth Induced by Common Carotid Artery Occlusion

• DOI: 10.1161/strokeaha.107.503433
• 发表时间: 2008-06
• 期刊: Stroke
• 影响因子: 8.3
• 作者: K. Todo;K. Kitagawa;Tsutomu Sasaki;E. Omura-Matsuoka;Y. Terasaki;N. Oyama;Y. Yagita;M. Hori

Postischemic exercise decreases neurogenesis in the adult rat dentate gyrus

• DOI: 10.1016/j.neulet.2006.09.040
• 发表时间: 2006-11
• 期刊: Neuroscience Letters
• 影响因子: 2.5
• 作者: Y. Yagita;K. Kitagawa;Tsutomu Sasaki;Y. Terasaki;K. Todo;E. Omura-Matsuoka;M. Matsumoto;M. Hori

Bcl2 enhances survival of newborn neurons in the normal and ischemic hippocampus

• DOI: 10.1002/jnr.21036
• 发表时间: 2006-11-01
• 期刊: JOURNAL OF NEUROSCIENCE RESEARCH
• 影响因子: 4.2
• 作者: Sasaki, Tsutomu;Kitagawa, Kazuo;Hori, Masatsugu
• 通讯作者: Hori, Masatsugu