Role of KLF15 on Energy metabolic regulation

1. KLF15基因在肥胖发展过程中的表达我们已经证明，在ob/ob小鼠和高脂饮食小鼠的肥胖发展过程中，KLF15基因的表达明显下调。脂肪细胞特异性KLF15转基因小鼠的构建我们成功构建了ap2启动子下的脂肪细胞特异性KLF15转基因小鼠。KLF15在转基因1系和转基因3系小鼠的白色脂肪组织中的含量分别增加了1倍和3倍。脂肪细胞特异性KLF15转基因小鼠正常咀嚼饮食代谢分析。野生型小鼠和KLF15转基因小鼠的白色脂肪组织质量在正常咀嚼饲料中无显著差异。然而，KLF15转基因小鼠血浆脂联素浓度降低。脂肪细胞特异性KLF15转基因小鼠高脂肪饮食代谢分析高脂肪饮食增加野生型小鼠脂肪细胞质量。相比之下，维持高脂肪饮食中脂肪细胞特异性KLF15转基因小鼠的体重和脂肪细胞质量并未增加。此外，口服糖耐量试验显示，脂肪细胞特异性KLF15转基因小鼠具有显著的糖耐量。脂肪细胞特异性KIF15转基因ob/ob小鼠的代谢分析我们产生了KLF15脂肪特异性过表达的ob/ob小鼠。发现脂肪细胞特异性过表达KLF15的ob/ob小鼠的ob/ob值小于其ob/ob值。这种体重上的差异至少部分是由于前者动物的白色脂肪组织较小。KLF15在SCD1表达中的作用饲喂高脂饲料导致白色脂肪组织中SCD1含量显著上调。在脂肪细胞特异性KLF15转基因小鼠中未观察到肥胖发展过程中SCD1的上调。综上所述，KLF15通过表达SCD1/调控脂肪细胞肥大

1. KLF15 expression during the development of obesityWe have shown that the expression of the KLF15 gene is markedly down-regulated during the development of obesity in ob/ob mice and high fat-diet mice.2. Construction of adipocyte-specific KLF15 transgenic miceWe have made the 'adipocyte-specific KLF15 transgenic mice under the aP2-promoter. The amounts of KLF15 were increased by an 〜1-fold or 〜3-fold increase in white adipose tissue in line 1 or line 3 transgenic mice, respectively3. Metabolic analysis in adipocyte-specific KLF15 transgenic mice fed a normal chaw diet. The mass of white adipose tissue did not differ significantly between wild-type mice and KLF15 transgenic mice fed a normal chaw diet. However, the plasma concentration of adiponectin decreased in KLF15 transgenic mice.4. Metabolic analysis in adipocyte-specific KLF15 transgenic mice fed a high fat dietThe high fat diet increased the adipocyte mass in wild-type mice. In contrast, maintain of adipocyte-specific KLF15 transgenic mice on the high fat diet did not increase body weight and adipocyte mass. Moreover, an oral glucose tolerance test revealed marked glucose tolerance in adipocyte-specific KLF15 transgenic mice.5. Metabolic analysis in adipocyte-specific KIF15 transgenic ob/ob miceWe generated ob/ob mice with adipocyte-specific overexpression of KLF15. ob/ob mice with adipocyte-specific overexpression of KLF15 were found to smaller the their ob/ob litermates. This difference in body weight was attributable at least in part to smaller white adipose tissue in the former animals.6. Role of KLF15 on SCD1 expressionFeeding with the high fat diet resulted in a marked up-regulation of the amount of SCD1 in white adipose tissue. This up-regulation of SCD1 during the development of obesity was not observed in adipocyte-specific KLF15 transgenic mice. In conclusion, KLF15 regulates adipocyte hypertrophy via the expression of SCD1/