Seeding of Vascular Grafts with Adipose Derived Adult Stem (ADAS) Cells

The long-term patency for infrapopliteal bypasses is not favorable in the case using a synthetic vascular graft. The main cause of the synthetic graft failure is that the pseudo-intima of the vascular graft is not covered with regenerated endothelial cells, which results to graft thrombosis in the early stage, or intirnal hyperplasia of the anasotmoses in the late stage. For endothelialization of the graft intima, seeding vascular grafts with endothelial cells had been developed. However, mature endothelial cells have a low proliferative capacity. Thus, seeding vascular grafts with endothelial cells has not been used in the usual clinical settings. Adipose錨erived adult stem (ADAS) cell is a somatic stem cell including fat tissue. ADAS cells are pluripotent cells to differenciate mesenchmal cells, like as fat, bone, muscle, cartilage, and abundantly exist in tissue. Recently, it was reported that ADAS cells can be diffrenciated to endothelial cells. Therefore, endothelialized ADAS culls may be an ideal cell source for a small caliber seeding synthetic graft for infrapopliteal bypass. The purpose of the present study was to develop a seeding vascular graft with ADAS cells. The tissue fragments, which were aspirated from rat subcutaneous adipose tissue, were incubated with cellagenase, and the digest was centrifuged, thereby separating the floating population of mature adipocytes from ADAS cells. Passage culture of ADAS cells was done, and we confirmed that ADAS cells had a high proliferative capacity until 5^<th> passage. After 2 or more passages in cultutu, the expression of endothelial immunophenotype has been investigated in the surface of ADAS cells. Also, we are measuring nitric oxide or prostacyclin production from ADAS cells.

The long-term patency for infrapopliteal bypasses is not favorable in the case using a synthetic vascular graft. The main cause of the synthetic graft failure is that the pseudo-intima of the vascular graft is not covered with regenerated endothelial cells, which results to graft thrombosis in the early stage, or intirnal hyperplasia of the anasotmoses in the late stage. For endothelialization of the graft intima, seeding vascular grafts with endothelial cells had been developed. However, mature endothelial cells have a low proliferative capacity. Thus, seeding vascular grafts with endothelial cells has not been used in the usual clinical settings. Adipose錨erived adult stem (ADAS) cell is a somatic stem cell including fat tissue. ADAS cells are pluripotent cells to differenciate mesenchmal cells, like as fat, bone, muscle, cartilage, and abundantly exist in tissue. Recently, it was reported that ADAS cells can be diffrenciated to endothelial cells. Therefore, endothelialized ADAS culls may be an ideal cell source for a small caliber seeding synthetic graft for infrapopliteal bypass. The purpose of the present study was to develop a seeding vascular graft with ADAS cells. The tissue fragments, which were aspirated from rat subcutaneous adipose tissue, were incubated with cellagenase, and the digest was centrifuged, thereby separating the floating population of mature adipocytes from ADAS cells. Passage culture of ADAS cells was done, and we confirmed that ADAS cells had a high proliferative capacity until 5^<th> passage. After 2 or more passages in cultutu, the expression of endothelial immunophenotype has been investigated in the surface of ADAS cells. Also, we are measuring nitric oxide or prostacyclin production from ADAS cells.