New Method of Cryopreservation for Pancreatic Islets by Flash Freeze Apparatus

闪冻仪冷冻保存胰岛的新方法

• 批准号: 18591426
• 负责人: AKUTSU Naotake
• 金额: $ 2.57万
• 依托单位: Clinical research Center, Chiba-East National Hospital, National Hospital Organization
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591426/
• 关键词: Transplantation, Regeneration; Cell, Tissue; 外科

Pancreatic islet transplantation has been performed for the patients with insulin-dependent diabetes mellitus (IDDM) in Japan since 2004. However, the treatment has not yet been popular, because donor for islet transplantation has been very few. We are not able to transplant the islets if enough population of islet is not isolated from donor pancreas. In the case, we should do cryopreservation of islet for using it in future chance of transplantation. Cryopreservation of pancreatic islet has been developed in several years. However, function and recovery rate of islet after thawing are not fully satisfied. More studies are necessary to apply to clinical treatment. In this research, we searched for a solution of better cryopreservation methods for pancreatic islet.We developed a new apparatus for cryopreservation of pancreatic islet together with the Islet Preservation Team in Showa Tansan Co., LTD. The apparatus uniformly makes freeze particles of islet in a moment by mixing islet particles and liquid nitrogen. Consequently, freeze islets are thought to keep the structure and secretion function of islet after thawed out. With this apparatus, we were able to constantly prepare 0.5mm diameter freeze particles of CP-1/RPMI1640 freeze liquid and get the particles with 80% of collecting rate. However, with colloid solution of islets, freeze particles were not sufficiently made and the freeze islet particles were not inconveniently rescued from the apparatus. Now, the apparatus has been improved to overcome these problems.This apparatus would be a potent instrument for solution of several problems in cryopreservation of pancreatic islets.

自2004年以来，胰岛移植已在日本被用于治疗胰岛素依赖型糖尿病（IDDM）患者。然而，由于胰岛移植的供体很少，这种治疗方法尚未普及。如果不能从供体胰腺中分离出足够数量的胰岛，则无法进行胰岛移植。在这种情况下，我们应该对胰岛进行冷冻保存，以备将来移植时使用。胰岛的低温保存是近年来发展起来的。然而，解冻后胰岛的功能和恢复速度并不完全令人满意。需要更多的研究来应用于临床治疗。在本研究中，我们寻找一种更好的胰岛冷冻保存方法。我们与昭和Tansan株式会社的胰岛保存小组共同开发了一种新的胰岛冷冻保存装置。该装置通过将胰岛颗粒与液氮混合，在瞬间均匀地使胰岛颗粒冻结。因此，冷冻胰岛被认为在解冻后保持了胰岛的结构和分泌功能。利用该装置，我们可以连续制备直径0.5mm的CP-1/RPMI1640冷冻液的冷冻颗粒，并以80%的收集率获得颗粒。然而，胰岛的胶体溶液不能充分地制造出冷冻颗粒，也不能很方便地将冷冻的胰岛颗粒从仪器中解救出来。现在，为了克服这些问题，仪器得到了改进。该装置将成为解决胰岛低温保存中若干问题的有效工具。

项目成果

Acute cellular rejection occurring in the first day after living-related renal transplantation : a case repotr.

活体肾移植后第一天发生的急性细胞排斥反应：病例报告。

• 发表时间: 2006
• 期刊: Clin Transplant 20
• 作者: Maruyam M.;Kenmochi T.;Saigo K.;Akutsu N.;Iwashita C.;Ohtsuki K.;Kitamura H.;Joh K.
• 通讯作者: Joh K.

分離膵島の機能評価

离体胰岛的功能评估

• 发表时间: 2006
• 期刊: Organ Biology 13.2
• 作者: 丸山 通広;剣持 敬;西郷 健一;圷 尚武 他
• 通讯作者: 圷 尚武 他

Two Cases of Calcineurin Inhibitor-Associated Reversible Posterior Leuk oencephaloathy Syndrome in Renal Translant Reciients

肾移植受者钙调神经磷酸酶抑制剂相关可逆性后白细胞脑病综合征两例

• 发表时间: 2007
• 作者: 丸山 通広;剣持 敬;大月 和宣;Akutsu N;Akutsu N
• 通讯作者: Akutsu N

Two cases of APS.

APS 两例。

• 发表时间: 2007
• 作者: Akutsu N;Maruyama M;Saigo K;Iwashita C;Ootsuki K;Kenmochi T
• 通讯作者: Kenmochi T

Successful engraftment of cryopreserved human pancreatic islets into dialbetic nude mice.

冷冻保存的人胰岛成功植入糖尿病裸鼠体内。

• 发表时间: 2007
• 期刊: Low Temp Med 33
• 作者: Kenmochi T.;Maruyam M.;Mullen Y.;Saigo K.;Akutsu N.;Iwashita C.;Ohtsuki K.;Suzuki A.;Miyazaki M.
• 通讯作者: Miyazaki M.