Correlation of motility and neuronal characteristics in intestinal allograft chronic rejection

同种异体肠慢性排斥反应中运动与神经元特征的相关性

• 批准号: 18591422
• 负责人: HOSHINO Ken
• 金额: $ 2.53万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591422/
• 关键词: small bowel transplantation; chronic rejection; motility; neuronal cell; interstitial cells of Cajal

【Aim】The aim of this study was to elucidate correlation between motility and neuronal integrity for chronic rejection after small bowel transplantation in rats.【Material and Method】Orthotopic SBTx was performed and recipients were divided following three groups; group 1: Allogenic SBTx from Brown Norway (BN) to Lewis (LEW) was done with FK506 at a dose of 2mg/kg, intramuscularly, day0-5, which is considered as chronic rejection model. group 2: Allogenic SBTx was also done with FK506 at a dose of 2mg/kg i.m., day0-9, which served as long-term functional model. group 3: Syngenic transplant was prepared from BN to BN without FK506. On day 80, three miniature strain gauge force transducers were set on the graft to evaluate motility pattern in vivo. Wholemount of the grafts were applied into cuprolinic blue staining and immunohistochemistry to assess population of neuronal cell and interstitial cells of Cajal (ICC). H-E histological examination of the intestinal grafts was also performed to … More define the chronic rejection.【Results】Histology of the Intestinal GraftsIn group 1, arterial wall thickening as vascular sclerotic findings and thickening in the muscular layer was observed in H-E staining which was fit in the definition of chronic rejection. Whereas the other 2 groups had no finding of rejection.Motility of the Intestinal GraftsThe parameter of MMC phase III has a characteristic alteration among these groups. The duration and interval of MMCphase III has a significant difference between group 1 and group 3. (syngenic model vs chronic rejection modelDuration ; 6.2±1.8 (min) vs 5.7±1.7 (min) (p<0.01), Interval ; 19.1±5.3(min) vs 16.7±4.3(min) (p<0.0001))Labeling-neuronal cells and ICCWe are still under investigation.[Conclusion] This study provides important characteristics on motility in chronically rejected intestinal allogtafts. The motility parameter including duration and interval of migrating motor complex indicates a key role for diagnosing chronic rejection. We are still under investigation on the alteration of the neuronal condition, but we believe these approaches will reveal the correlation with dysmotility of the grafts. Less

【目的】本研究旨在探讨大鼠小肠移植后慢性排斥反应的运动与神经元完整性的关系。【材料与方法】行原位SBTx，受者分为三组；1组：来自Brown Norway （BN）到Lewis （LEW）的同种异体SBTx用FK506给药，剂量为2mg/kg，肌注，第0-5天，视为慢性排斥模型。2组：同种异体SBTx也与FK506一起以2mg/kg i.m，第0-9天，作为长期功能模型。第3组：将BN同基因移植至不含FK506的BN。第80天，在移植物上放置3个微型应变式力传感器，以评估移植物在体内的运动模式。整块移植物经丙二酚蓝染色和免疫组化检测Cajal （ICC）神经细胞和间质细胞的数量。同时对移植物进行H-E组织学检查，进一步明确慢性排斥反应。【结果】1组大鼠动脉壁呈血管硬化样增厚，H-E染色见肌层增厚，符合慢性排斥反应的定义。而另外两组没有发现排斥反应。肠移植物的移动性MMC III期参数在这些组中有特征性的改变。mmciii期持续时间和间隔在1组和3组之间有显著差异。持续时间：6.2±1.8 (min) vs 5.7±1.7 (min) (p<0.01)，时间间隔；19.1±5.3(min) vs 16.7±4.3（min) (p<0.0001)）标记-神经元细胞和ICCWe仍在研究中。[结论]本研究提供了慢性排斥肠异体运动的重要特征。运动参数包括运动复合体迁移的持续时间和时间间隔，在慢性排斥反应的诊断中具有关键作用。我们仍在研究神经元状况的改变，但我们相信这些方法将揭示移植物运动障碍的相关性。少