Establishment on early detection of acute cellular rejection in small bowel transplantation using motility evaluation technique

运动评估技术早期检测小肠移植急性细胞排斥反应的建立

• 批准号: 12671174
• 负责人: HOSHINO Ken
• 金额: $ 2.24万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671174/
• 关键词: strain gauge force transducer; motility; small bowel transplantation; rat; Motility; strain gauge force transducer; 腸管運動

Experiment 1: Evaluation of motility of the small intestine in normal controls.1-(A)- by implanted transducer.[Method] An implanted type of strain gauge transducer which was located in the abdominal cavity was sutured at the gastric antrum, duodenum and jejunum in 5 Wistar rats. [Result] The rats survived from 0 day to 5days(mean 2.2 days). By autopsy, it was suggested that cause of death was the obstruction due to the size of the implanted transducer. [Discussion] We consider that it is difficult to apply implanted transducer to the rats in this stage.1-(B)- by the way of direct connection to the PCs.[Method] 3 transducer were sutured to the jejunum every 5cm. The wire was induced into the subcutaneously to the back. Transducers were directly connected to the installed computer (n=10). [Result] Migrating Motor Complex was observed in the fasting period. The parameter of duration, interval, amplitude was 3.6±0.96 (min), 6.8±2.4(min), 55.7±6.3(x1/1000(g)), respectively. [Discussion] We … More established the evaluation system of the rats intestinal motility in a way of directly connected manner.Experiment 2: Evaluation of motility of the small intestine in the operated rats.[Method] 15cm of jejunum was transected as Thiry-Vella loop with vasculature and the remain of the intestine was reanastomosed. 3 transducerwere sutured to the Thiry-Vella loop every 5cm apart in 9 Wistar rats. [Result] We successfully confirmed the Migrating Motor Complex in the fasting period. The parameter of duration, interval, amplitude was 4.0±0.8(min), 4.4±1.4(min), 83.2±21.1 (x1/1000(g)), respectively. [Discussion]The method we've established to evaluate the intestinal motility was able to apply to the post-surgical model in rats.Experiment 3: Evaluation of motility of the small intestine in the transplanted rats.[Method] Group I: Syngenic model: LEW to LEW small bowel transplantation was done heterotopically. Group II: Allogenic model: BN to LEW small bowel transplantation was done with immunosupression(FK506 0.64mg/kg/d) Group III: BN to LEW small bowel transplantation was done without immunosupression Three transducers were sutured to the transplanted grafts as Thiry-Vella loop every 5cm apart. [Result] In group I, high amplitude non-propagative contraction was seen in day 5, however, migrating motor complex was observed in day 7. The parameter of duration, interval, amplitude was 7.2±2.0(min), 11.2±2.3(min), 119.9±35.8 (x1/1000(g)), respectively. In group II, MMC was appeareded in day 10, whereas no MMC was seen in day 7. In group III, almost no contraction was seen during all the periods. [Discussion] We considerthat evaluation of the transplanted grafts is thought to be one mean of assessing rejection in stead of histological examination. Less

实验1：正常对照小肠运动的评价。1-（A）-通过植入换能器。[方法]将植入式应变传感器置于5只Wistar大鼠的腹腔内，分别缝合于胃窦、十二指肠和空肠。[结果]大鼠存活0~5d，平均2.2d。通过尸检，表明死亡原因是由于植入换能器的尺寸引起的阻塞。[讨论]我们认为在这个阶段很难将植入式换能器应用于大鼠。1-（B）-通过直接连接到PC的方式。[方法]将3个传感器每隔5cm与空肠缝合。将导丝导入背部皮下。传感器直接连接到安装的计算机（n = 10）。[结果]禁食期出现移行性复合运动。持续时间、间期、波幅分别为3.6 ± 0.96（min）、6.8 ± 2.4（min）、55.7 ± 6.3（x1/1000（g））。[讨论]我们 ...更多信息 实验二：手术大鼠小肠运动功能的评价。[方法]切断空肠15cm，形成带血管蒂的Thiry-Vella袢，将剩余的肠管缝合。9只Wistar大鼠，每隔5cm将3个传感器缝合在Thiry-Vella环上。[结果]成功证实了禁食期的移行性复合运动。持续时间、间期、波幅分别为4.0 ± 0.8（min）、4.4 ± 1.4（min）、83.2 ± 21.1（x1/1000（g））。[讨论]本研究建立的小肠动力学评价方法可应用于大鼠小肠移植后模型。实验三：移植大鼠小肠动力学评价。[方法] I组：同系模型：采用LEW对LEW异位小肠移植。第二组：同种异体模型：第三组：BN-LEW小肠移植，不加免疫抑制剂（FK506 0.64mg/kg/d）。[结果] I组第5天出现高振幅非传播性收缩，第7天出现移行性复合运动。持续时间、间期、波幅分别为7.2 ± 2.0（min）、11.2 ± 2.3（min）、119.9 ± 35.8（x1/1000（g））。Ⅱ组第10天出现MMC，第7天未见MMC。在第三组，几乎没有收缩被认为是在所有的时期。[讨论]我们认为移植移植物的评价是评估排斥反应的一种手段，而不是组织学检查。少