Establishment on early detection of acute cellular rejection in small bowel transplantation using motility evaluation technique

实验一：评价正常对照组的小肠运动。1-(A)-埋藏式传感器。[方法]将埋藏式应变片传感器置于5只Wistar大鼠的胃窦、十二指肠和空肠缝合于胃窦、十二指肠和空肠。[结果]大鼠存活时间为0~5d，平均2.2d。通过尸检，死亡原因是由于植入的换能器的大小造成的梗阻。[讨论]我们认为植入换能器对现阶段的大鼠来说是困难的1-(B)-通过直接连接PC的方式。[方法]每隔5 cm在空肠缝合3个换能器。钢丝被引到背部的皮下。传感器直接连接到安装的计算机(n=10)。[结果]禁食期间观察到移行性运动复合体。时程、间期、波幅参数分别为3.6±0.96(Min)、6.8±2.4(Min)、55.7±6.3(x1/1000(G))。[讨论]We…实验2：手术大鼠小肠运动功能的评价。[方法]将15 cm的空肠横断成带血管蒂维拉环，将剩余的空肠重新吻合。取9只Wistar大鼠，每隔5 cm缝合3个探头至Thary-Vella环。[结果]我们成功地确认了禁食期间的移行性运动复合体。时程、间期、波幅参数分别为4.0±0.8(Min)、4.4±1.4(Min)、83.2±21.1(x1/1000(G))。[讨论]我们建立的评价肠运动功能的方法适用于大鼠术后模型。实验3：移植大鼠小肠运动功能的评价。[方法]I组：同种异位Lew to Lew小肠移植模型。II组：BN-Lew同种异体小肠移植模型：BN-Lew小肠移植加免疫抑制(FK506，0.64 mg/kg/d)；III组：BN-Lew小肠移植，不加免疫抑制，每隔5 cm缝合3个探头，形成Thry-Vella环。[结果]I组5d出现高波幅非传播性收缩，7d出现移行性运动复合体。时程、间期、波幅参数分别为7.2±2.0min、11.2±2.3min、119.9±35.8x1/1000(G)。II组在第10天出现MMC，第7天未见MMC。III组在所有时间段几乎未见收缩。[讨论]我们认为评估移植的移植物可以代替组织学检查作为评估排斥反应的一种手段。较少

Experiment 1: Evaluation of motility of the small intestine in normal controls.1-(A)- by implanted transducer.[Method] An implanted type of strain gauge transducer which was located in the abdominal cavity was sutured at the gastric antrum, duodenum and jejunum in 5 Wistar rats. [Result] The rats survived from 0 day to 5days(mean 2.2 days). By autopsy, it was suggested that cause of death was the obstruction due to the size of the implanted transducer. [Discussion] We consider that it is difficult to apply implanted transducer to the rats in this stage.1-(B)- by the way of direct connection to the PCs.[Method] 3 transducer were sutured to the jejunum every 5cm. The wire was induced into the subcutaneously to the back. Transducers were directly connected to the installed computer (n=10). [Result] Migrating Motor Complex was observed in the fasting period. The parameter of duration, interval, amplitude was 3.6±0.96 (min), 6.8±2.4(min), 55.7±6.3(x1/1000(g)), respectively. [Discussion] We … More established the evaluation system of the rats intestinal motility in a way of directly connected manner.Experiment 2: Evaluation of motility of the small intestine in the operated rats.[Method] 15cm of jejunum was transected as Thiry-Vella loop with vasculature and the remain of the intestine was reanastomosed. 3 transducerwere sutured to the Thiry-Vella loop every 5cm apart in 9 Wistar rats. [Result] We successfully confirmed the Migrating Motor Complex in the fasting period. The parameter of duration, interval, amplitude was 4.0±0.8(min), 4.4±1.4(min), 83.2±21.1 (x1/1000(g)), respectively. [Discussion]The method we've established to evaluate the intestinal motility was able to apply to the post-surgical model in rats.Experiment 3: Evaluation of motility of the small intestine in the transplanted rats.[Method] Group I: Syngenic model: LEW to LEW small bowel transplantation was done heterotopically. Group II: Allogenic model: BN to LEW small bowel transplantation was done with immunosupression(FK506 0.64mg/kg/d) Group III: BN to LEW small bowel transplantation was done without immunosupression Three transducers were sutured to the transplanted grafts as Thiry-Vella loop every 5cm apart. [Result] In group I, high amplitude non-propagative contraction was seen in day 5, however, migrating motor complex was observed in day 7. The parameter of duration, interval, amplitude was 7.2±2.0(min), 11.2±2.3(min), 119.9±35.8 (x1/1000(g)), respectively. In group II, MMC was appeareded in day 10, whereas no MMC was seen in day 7. In group III, almost no contraction was seen during all the periods. [Discussion] We considerthat evaluation of the transplanted grafts is thought to be one mean of assessing rejection in stead of histological examination. Less