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The clarification and control of the mechanism. concerning metastasis and invasion in colorectal cancer.

机制的明确和控制。

基本信息

批准号：
18591473
负责人：
SATO Mikinori
金额：
$ 2.6万
依托单位：
Nagoya City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591473/
关键词：
adhesive molecule metastasis and invasion colorectal cancer transcrintional factor

项目摘要

In this study, we examined the role of c- kit receptor (KIT) signal transduction on the proliferation and invasion of colorectal cancer cells. We found that c- kit was expressed in two colorectal cancer cell lines as determined by RT-PCR, Western blot and flow cytometry. In KIT-positive lines, KIT was activated by stem cell factor (SCF). SCF enhanced cellular proliferation of positive lines as demonstrated by the WST-1 proliferation assay. Furthermore, SCF enhanced the invasive ability of KIT-positive cell lines. SCF stimulation upregulated p44/42 mitogen-activated protein kinase (MAPK) and Akt as shown by Western blot. We examined the roles played by p44/42 MAPK and phosphatidylinositol 3-kinase (PI3K)/Akt pathways in proliferation and invasion. PI3K/Akt activity strongly correlated with proliferation and invasion while p44/42 MAPK was correlated with only invasion. In conclusion, the SCF-enhanced proliferation and invasion of KIT-positive colorectal cancer cells is achieved mainly through the PI3K/Akt pathway.

本研究旨在探讨c-kit受体（KIT）信号转导在大肠癌细胞增殖和侵袭中的作用。RT-PCR、Western blot和流式细胞仪检测结果表明，两种大肠癌细胞系均表达c-kit。在KIT阳性细胞系中，KIT被干细胞因子（SCF）激活。WST-1细胞增殖实验表明SCF能促进阳性细胞系的增殖。此外，SCF增强了KIT阳性细胞系的侵袭能力。Western blot显示SCF刺激可上调p44/42丝裂原活化蛋白激酶（MAPK）和Akt表达。我们研究了p44/42 MAPK和磷脂酰肌醇3-激酶（PI 3 K）/Akt通路在增殖和侵袭中所起的作用。PI 3 K/Akt活性与细胞增殖和侵袭密切相关，而p44/42 MAPK活性仅与细胞侵袭相关。结论：SCF增强KIT阳性结直肠癌细胞的增殖和侵袭主要通过PI 3 K/Akt途径实现。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

The stem cell factor/c-kit receptor pathway enhances proliferation and invasion of pancreatic cancer cells.

干细胞因子/C-KIT受体途径增强了胰腺癌细胞的增殖和侵袭。

DOI：
10.1186/1476-4598-5-46
发表时间：
2006-10-18
期刊：
Molecular cancer
影响因子：
37.3
作者：
通讯作者：

Reactive Lymphoid Hyperplasia of the liver in a patient with colon cancer: report of two cases

DOI：
10.1186/1471-230x-6-25
发表时间：
2006-09-12
期刊：
BMC GASTROENTEROLOGY
影响因子：
2.4
作者：
Takahashi, Hiroki;Sawai, Hirozumi;Manabe, Tadao
通讯作者：
Manabe, Tadao

Stem Cell Factor/c-kit Receptor Signaling Enhances the Proliferation and Invasion of Colorectal Cancer Cells Through the PI3K/Akt Pathway

DOI：
10.1007/s10620-007-9759-7
发表时间：
2007-04
期刊：
Digestive Diseases and Sciences
影响因子：
3.1
作者：
Akira Yasuda;H. Sawai;Hiroki Takahashi;N. Ochi;Y. Matsuo;H. Funahashi;Mikinori Sato;Y. Okada;H. Takeyama;T. Manabe
通讯作者：
Akira Yasuda;H. Sawai;Hiroki Takahashi;N. Ochi;Y. Matsuo;H. Funahashi;Mikinori Sato;Y. Okada;H. Takeyama;T. Manabe

A new technique for intestinal isoperistaltic anastomosis utilizing a linear stapler for enlargement after anastomosis performed with a circular stapler

环形吻合器吻合后线性吻合器扩大肠道等蠕动吻合新技术