Histological damage oflung allografts according to the magnitude of acute rejection in the reiso-transplant model

根据 reiso 移植模型中急性排斥程度的同种异体肺移植物的组织学损伤

• 批准号: 18591543
• 负责人: IWATA Hisashi
• 金额: $ 2.4万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591543/
• 关键词: Pulmonary surgery; lune transnlantation; lung regeneration; 拒絶反応

ABSTRACTBackground: Graft damage due to acute rejection has been reported to be one of the risk factors in the chronic stage of cardiac and renal allografts. This study was designed to elucidate the histological changes of grafts after ongoing acute allograft rejection was discontinued in lung re-iso-transplantation models.Methods: WKAH rat lungs were orthotopically transplanted into F344 recipients. Three days (Group 3A) and 5 days (Group 5A) after the first allotransplantation, the grafts were re-iso-transplanted back into the WKAH rats (Group 3RA and Group 5RA, respectively). Five days (Group 5I) after the first isotransplantation, the grafts were re-iso-transplanted back into the WKAH rats (Group 5RI). The grafts were removed 30 and 60 days after re-iso-transplantation and assessed histologically.Results: Typical acute allograft rejection developed in the 3A and 5A groups, and the changes were reduced after re-iso-transplantation, though they remained significantly greater in the 5RA group than in the 3RA and SRI groups. In intimal hyperplasia, the score of the graft 60 days after re-iso-transplantation in the 5RA group was significantly higher than in the SRI and 3RA groups. The changes in airway inflammation were significantly greater in the 5RA group than in the 3RA and SRI groups at 60 days. Peribronchiolar fibrosis was significantly greater in the 5RA and 3RA groups than in the SRI group.Conclusions: Acute rejection and airway inflammation corresponded to the magnitude of rejection before retransplantation; significant intimal hyperplasia developed in severe acute rejection, and peribronchiolar fibrosis occurred after the first acute rejection.

背景：急性排斥反应引起的移植物损伤已被报道为心脏和肾移植慢性期的危险因素之一。本研究旨在阐明肺再同种异体移植模型中持续急性排斥反应终止后移植物的组织学变化。方法：WKAH大鼠肺原位移植至F344受体。第一次同种异体移植后3d(3A组)和5d(5A组)将移植物重新等位移植回WKAH大鼠(3RA组和5RA组)。第一次同种异体移植后5天(5I组)，将移植物重新等同移植回WKAH大鼠体内(5RI组)。结果：3A组和5A组均出现典型的急性排斥反应，移植后3A组和5A组的急性排斥反应明显减轻，5RA组的急性排斥反应程度明显高于3RA组和SRI组。在内膜增生方面，5RA组移植后60d的移植物评分显著高于SRI组和3RA组。60d时，5RA组的气道炎症改变明显大于3RA组和SRI组。5RA组和3RA组细支气管周纤维化程度明显高于SRI组。结论：急性排斥反应和气道炎症与再次移植前的排斥反应程度相对应，重度急性排斥反应时发生明显的内膜增生，首次急性排斥反应后出现细支气管周纤维化。

项目成果

Histological damage of lung allografts according to the magnitude of acute rejection in the re-iso-transplant model

根据再异移植模型中急性排斥程度的同种异体肺移植物的组织学损伤

• 期刊: Journal of Heart and Lung Transplantation In Press
• 作者: Kyoichi Takaori;Tomoyuki Agui;Yoshiharu Miyamoto;Mitsuhiko Iwamoto;Hisashi Watanabe;Nobuhiko Tanigawa;Yutaro Egashira;Suguru Yonezawa.;Tsutomu Marui
• 通讯作者: Tsutomu Marui