A study of target recognition and axon branching m hanisms in the thalamomrocalpmjection

丘脑注射中目标识别和轴突分支机制的研究

• 批准号: 18300105
• 负责人: YAMAMOTO Nobuhiko
• 金额: $ 10.75万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18300105/
• 关键词: neocorex; thalamus; axon branching; layer; neural activity; 層構造

The thalamocortical (TC) projection in the mammalian brain is a well characterized system in terms of laminar specificity of neocortical circuits. To understand the mechanisms that underlie lamina-specific TC axon targeting, the role of extracellular and cell surface molecules which are expressed in the upper layers of the developing cortex was studied in vitro culture techniques. The result demonstrated that multiple upper layer molecules cooperated to produce stop behavior of TC axons in the target layer. Activity dependency of TC axon branching was also investigated in organotypic cocultures of the thalamus and cortex. TC axon branches were formed dynamically by addition and elimination during the second week in vitro, when spontaneous firing increased in thalamic and cortical cells. Pharmacological blockade of firing or synaptic activity reduced the remodeling process, in particular, branch addition in the target layer. Together, these findings suggest that TC axon targeting mechanisms involve the regulation with multiple lamina-specific molecules and modification of the molecular mechanisms via neural activity.

哺乳动物大脑中的丘脑皮质（TC）投射是一个很好的表征系统，在新皮层回路的层状特异性。为了了解板层特异性TC轴突靶向的机制，在体外培养技术中研究了在发育皮层的上层中表达的细胞外和细胞表面分子的作用。结果表明，多个上层分子协同作用，产生了靶层TC轴突的停止行为。TC轴突分支的活动依赖性也进行了研究，在丘脑和皮质的器官型共培养。TC轴突分支的形成动态添加和消除在第二周在体外，当自发放电增加在丘脑和皮层细胞。药理学阻断放电或突触活动减少了重塑过程，特别是靶层中的分支增加。总之，这些发现表明，TC轴突靶向机制涉及多个板层特异性分子的调节和通过神经活动的分子机制的修改。

项目成果

Activity-independent and dependent mechanisms for the formation of the thalamocortical projection

丘脑皮质投射形成的活动独立和依赖性机制

• 发表时间: 2007
• 作者: Yamamoto;N.;Uesaka;N.;Hayano;Y.;Yamada;A
• 通讯作者: A

Cooperative activity of multiple upper layer proteins for thalamocortical axon growth

• DOI: 10.1002/dneu.20592
• 发表时间: 2008-02-15
• 期刊: DEVELOPMENTAL NEUROBIOLOGY
• 影响因子: 3
• 作者: Maruyama, Takuro;Matsuura, Masahiro;Yamamoto, Nobuhiko
• 通讯作者: Yamamoto, Nobuhiko

Activity-dependent regulation of cortical axon branching

皮质轴突分支的活动依赖性调节

• 发表时间: 2006
• 期刊: Neuroscientist 12
• 作者: Uesaka N.;Yamamoto N
• 通讯作者: Yamamoto N

大脳皮質神経細胞におけるHDAC9の神経活動依存的な核・細胞質間移動による遺伝子発現と樹状突起形成の制御

大脑皮层神经元中 HDAC9 神经元活动依赖性核细胞质运动对基因表达和树突形成的调节

• 发表时间: 2007
• 作者: Sugo;N・Oshiro;H・Song;W・Yamamoto;N
• 通讯作者: N

The role of neural activity in cortical axon branching

神经活动在皮质轴突分支中的作用

• 发表时间: 2006
• 期刊: Neuroscientist 12
• 作者: Uesaka N;Ruthazer ES;Yamamoto N
• 通讯作者: Yamamoto N