The Influence of the Pretectum on the Visual Thalamus
前顶盖对视觉丘脑的影响
基本信息
- 批准号:10748541
- 负责人:
- 金额:$ 55.68万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2023
- 资助国家:美国
- 起止时间:2023-09-01 至 2026-07-31
- 项目状态:未结题
- 来源:
- 关键词:AddressAffectAmblyopiaAnimalsAreaBehaviorBehavioralBrainButyric AcidsCalcium SignalingCell NucleusCellsClassificationComplexDarknessDepth PerceptionDiagnosisDiseaseDisinhibitionDorsalDyslexiaElectron MicroscopyEye MovementsFelis catusFluorescenceGeneticGlutamatesImageIn VitroInjectionsInterneuronsKnowledgeLabelLasersLateral Dorsal NucleusLateral Geniculate BodyLightLinkMammalsMediatingMedicalMethodsMiosis disorderModelingMorphologyMotionMotion PerceptionMotorMovementMusNeuronsNeurotransmittersOcular Motility DisordersParvalbuminsPathologic NystagmusPathway interactionsPatternPerceptionPlayPopulationPropertyPulvinar structureResearchResearch PersonnelRetinaRetinal Ganglion CellsRoleRunningSaccadesSchizophreniaSensoryShapesSignal TransductionSourceSpeedStrabismusSynapsesTechniquesTestingThalamic structureTransgenic OrganismsViralViral VectorVisionVisualVisual MotionWhole-Cell Recordingsactive visionarea striataawakecalcium indicatorcell typeextracellularganglion cellgazein vivoinsightmonocularnoveloptogeneticsrabies viral tracingreceptive fieldresponseretinogeniculatetooltransmission processtwo-photonvisual stimulusvisual tracking
项目摘要
PROJECT SUMMARY / ABSTRACT
The pretectum (PT) is a relatively unexplored brain area in mammals, and it is currently unknown
how the PT affects the visual properties of thalamic neurons that underly the complex integration
of vision and movement. Here, a team of investigators propose a multifaceted approach - from
synapse to behavior – to answer fundamental questions regarding PT circuits (Aim 1), the visual
and/or motor response properties of identified PT neurons (Aim 2), the effects of PT input on the
response properties of thalamic neurons (Aim 3), and the effects of the PT on visual behavior
(Aim 4). Each aim will utilize a combination of novel intersectional circuit labeling techniques,
neuronal activation, and recording strategies to probe two identified PT pathways: a GABAergic
pathway to the lateral geniculate nucleus (LGN) and the visual sector of the thalamic reticular
nucleus (vTRN), and a nonGABAergic pathway to the pulvinar nucleus (PUL) from PT neurons
that contain parvalbumin. Aim 1 will use cre-dependent monosynaptic rabies tracing to induce
the expression of the calcium indicator GCaMP to classify, using 2P imaging, the receptive field
properties and morphology of retinal ganglion cells that innervate PT-LGN/TRN and PT-PUL
neurons. Monosynaptic rabies tracing will also be used to characterize cortical and subcortical
brain inputs to PT-LGN/TRN and PT-PUL cells; identified PT inputs will subsequently be studied
using electron microscopy and in vitro whole cell recordings paired with optogenetic activation to
characterize their ultrastructure and synaptic properties. Aim 2 will use in vivo extracellular
recordings in awake animals paired with “opto-tagging” to determine the response properties of
PT-LGN/TRN and PT-PUL neurons. Aim 3 will use in vivo extracellular recordings from awake
animals paired with optogenetic activation of PT inputs to determine the effects of the PT inputs
on LGN, vTRN and PUL response properties. Finally, Aim 4 will use cre- and flp-dependent
chemogenetic activation of PT-LGN/TRN or PT-PUL neurons to determine if these cells affect
stereopsis and/or optokinetic responses. Collectively, these 4 lines of inquiry will provide key
information regarding the role of the PT in active vision.
项目摘要/摘要
顶盖前区(PT)是哺乳动物中相对未被探索的大脑区域,目前尚不清楚
PT如何影响复杂整合下丘脑神经元的视觉特性
视觉和运动的能力。在这里,一个调查小组提出了一个多方面的方法-从
突触行为-回答有关PT电路的基本问题(目标1),视觉
和/或运动反应特性的识别PT神经元(目的2),PT输入的影响,
丘脑神经元的反应特性(目的3),以及PT对视觉行为的影响
(Aim 4).每个目标将利用新的交叉电路标记技术的组合,
神经元激活和记录策略,以探测两个确定的PT途径:GABA能
外侧膝状体核(LGN)和丘脑网状核视觉区的通路
核(vTRN),以及从PT神经元到枕核(PUL)的非GABA能通路
含有小清蛋白目的1将使用依赖cre的单突触狂犬病示踪诱导
钙指示剂GCaMP的表达,使用2 P成像,感受野
支配PT-LGN/TRN和PT-PUL的视网膜神经节细胞的性质和形态
神经元单突触狂犬病示踪也将用于表征皮质和皮质下
PT-LGN/TRN和PT-PUL细胞的脑输入;随后将研究识别的PT输入
使用电子显微镜和体外全细胞记录与光遗传学激活配对,
表征它们的超微结构和突触特性。Aim 2将使用体内细胞外
记录在清醒的动物配对“光标记”,以确定响应特性
PT-LGN/TRN和PT-PUL神经元。Aim 3将使用清醒时的体内细胞外记录
与PT输入的光遗传学激活配对的动物,以确定PT输入的影响
LGN、vTRN和PUL响应特性。最后,目标4将使用cre和flp依赖
PT-LGN/TRN或PT-PUL神经元的化学发生激活,以确定这些细胞是否影响
立体视觉和/或视动反应。总的来说,这4条调查线将提供关键的
关于PT在主动视觉中的作用的信息。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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MARTHA E BICKFORD其他文献
MARTHA E BICKFORD的其他文献
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{{ truncateString('MARTHA E BICKFORD', 18)}}的其他基金
Midbrain Circuits for Perceptual Decision-Making
用于感知决策的中脑回路
- 批准号:
10792130 - 财政年份:2023
- 资助金额:
$ 55.68万 - 项目类别:
Midbrain circuits for perceptual decision-making
用于感知决策的中脑回路
- 批准号:
10216482 - 财政年份:2021
- 资助金额:
$ 55.68万 - 项目类别:
KY INBRE Admin Supplement: The Role of Platinum Leaving Ligands in Chemo-immunotherapeutic Resistance in Lung Cancer Models
KY INBRE 管理补充:铂离去配体在肺癌模型化疗免疫治疗耐药中的作用
- 批准号:
10399834 - 财政年份:2021
- 资助金额:
$ 55.68万 - 项目类别:
GABAergic circuit interactions within the behaving mouse dLGN
行为小鼠 dLGN 内 GABA 能电路的相互作用
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9449526 - 财政年份:2017
- 资助金额:
$ 55.68万 - 项目类别:
GABAergic circuit interactions within the behaving mouse dLGN
行为小鼠 dLGN 内 GABA 能电路的相互作用
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9568835 - 财政年份:2017
- 资助金额:
$ 55.68万 - 项目类别:
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