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Study of genetic of atomic bomb radiation by using high density DNA microarray based-CGH

基于高密度DNA微阵列的CGH研究原子弹辐射遗传学

基本信息

批准号：
18310043
负责人：
TAKAHASHI Norio
金额：
$ 11.32万
依托单位：
Radiation Effects Research Foundation
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18310043/
关键词：
genetics genome radiation microarray transgenerational effects

项目摘要

We have studied the trans-generational effects of A-bomb radiation on human germ cells, that is, whether the mutation rate has increased significantly in the offspring of A-bomb survivors compared with controls at genomic DNA level. To enable genome-wide detection of de novo mutation, we have introduced DNA-microarray based comparative genomic hybridization(so-called, array CGH) method. In order to establish the experimental conditions, we conducted a pilot study in which we examined 80 offspring of atomic-bomb suivivors. The results of the pilot study revealed that the experimental conditions established by us could effectively identify one-copy decrease and increase from normal diploidy. The summary paper has been published in the Annals of Human Genetics, 79, 193-204, 2008 in this year. Moreover, we have developed the computer software to effectively process the huge amount of data created from out array system. By using this improved methodology, we had examined 225 offspring born to atomic-bomb survivors exposed more than 1 Gy. The arrays containing about 2,500 BAG(Bacterial Artificial Chromosome) -clones were used in this study. The first population screening has been completed. We have identified many putative copy-number variants. These putative variants are expected to be good recourses for examining the trans-generational effects of radiation. We will have an opportunity to assess whether the rate of mutations observed in exposed group is higher than that in those from unexposed group.

我们研究了原子弹辐射对人类生殖细胞的跨代效应，即在基因组DNA水平上，原子弹幸存者后代的突变率是否比对照组显著增加。为了能够全基因组检测新生突变，我们引入了基于dna微阵列的比较基因组杂交（所谓的阵列CGH）方法。为了建立实验条件，我们进行了一项初步研究，对80名原子弹幸存者的后代进行了检查。中试结果表明，我们建立的实验条件可以有效地鉴定正常二倍体的单倍体减少和单倍体增加。摘要论文发表在《人类遗传学年鉴》（Annals of Human Genetics） 2008年第79期，193-204期。此外，我们已经开发了计算机软件，以有效地处理从阵列系统产生的大量数据。通过使用这种改进的方法，我们检查了受辐射超过1gy的原子弹幸存者所生的225个后代。本研究使用了包含约2500个细菌人工染色体（BAG）克隆的阵列。第一次人口普查已经完成。我们已经确定了许多假定的拷贝数变体。这些假定的变异有望成为检查辐射跨代效应的良好资源。我们将有机会评估暴露组观察到的突变率是否高于未暴露组。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Genome-wide single nucleotide polymorphism microarray mapping for prediction of outcome of neuroblastoma patients.

全基因组单核苷酸多态性微阵列图谱用于预测神经母细胞瘤患者的结果。

DOI：
发表时间：
2007
期刊：
影响因子：
0
作者：
Hiyama;E.
通讯作者：
E.

Prediction of individual response to platinum/paclitaxel combination using novel marker genes in ovarian cancels.

使用卵巢取消中的新标记基因预测个体对铂/紫杉醇组合的反应。

DOI：
发表时间：
2006
期刊：
Mol Cancer Ther 5(3)
影响因子：
0
作者：
萩原良巳;岡田憲夫;多々納裕一;Hashimoto Y;Kohara K;Mashimo Y;Nakamura H;Takahashi N;Tazaki T;Ueno T;Fumoto S;Hamasaki K;Hashimoto Y;Kohara K;Nakamura H;Takahashi N;Tazaki T;濱崎幹也;Nakamura H;Tazaki T;Arifin M;Hamasaki K;Hiyama E;Matsuo T;Otani K;Tanimoto K;Tomizawa M;Yunokawa M;Otani K;Tomizawa M;Otani K;Arifin M;Hamasaki K;Hiyama E;Matsuo T;Tanimoto K;Tomizawa M;Yunokawa M;Hasegawa T;Hiyama E;Iehara T;Komatsu M;Nakayama T;Omi T;Shimokuni T;高橋規郎;Yamaoka H;Yanada S;Hasegawa T;Komatsu M
通讯作者：
Komatsu M

DNAマイクロアレイcomparative genomic hybridization(アレイCGH)法を用いたゲノム解析;第6報。試行調査の結果

使用DNA微阵列比较基因组杂交（阵列CGH）方法进行基因组分析；第6份报告。