Construction of Database of Rat and Mouse Genes for Three Dimensional Monomer and Complex Protein Structure

点击翻译按钮获取中文摘要

In the first, we improved the performance of our Full Automatic Modeling System (FAMS) and Full Automatic Modeling System for protein complex (FAMS Complex). These modeling systems made it possible to predict accurate three-dimensional protein structures. The FAMS showed successful performance for prediction of protein three-dimensional structures in 7th Community Wide Experiment on the Critical Assessment of Techniques for Protein Structure Prediction (CASP7), which assessed the accuracy of modeling methods. Moreover the FAMS Complex performed well in Community wide experiment on the comparative evaluation of protein-protein docking for structure prediction (CAPRI).Secondarily, we developed the evaluation systems which evaluate quality of predicted protein structures and pairwise-alignments of amino acid sequences. These methods provide information of confidence of predicted models.Finally, we constructed huge protein structure modeling database of Rat and Mouse genes, and released as RIKEN FAMSBASE (http://famshelp.gsc.riken.jp/famsbase/) to the public. In the RIKEN FAMSBASE, any researchers can search three-dimensional protein structure models of Rat and Mouse genes from amino acid sequence, a Gene Name, and keywords.