Development of molecular memory system usingpeptide molecules

使用肽分子开发分子记忆系统

• 批准号: 18350063
• 负责人: KIMURA Shunsaku
• 金额: $ 10.18万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18350063/
• 关键词: self-assembly; helix peptide; cyclic peptide; dipole; STM; AFM; molecular device; molecular memory; 自己組織化膜; 走査トンネル顕微鏡; 分子メモリー; スイッチング

Thee helices were connected to a scaffold in a state where free rotation of the individual helix was allowed The peptide was spread on water, and the formed monolayer was transferred on gold STM image of the peptide on gold could not be obtained, probably because the peptides were not immobilized on the surface firmly. Then, the peptide was modified to possess a disulfide group at the N terminal of each helix. The modified peptide was incorporated into the self-assembled monolayer of dodecanethiolate. The modified peptide was observed successfully by STM. Notably, the modified peptide moved on gold in response to the applied bias voltage on the peptide. However, the peptide height remained constant. Anchoring the peptide via three points to gold should be the reason fix the constant height. On the other hand, peptide nanotubes were constructed by using cyclic β-peptides. Even though a single molecule image of cyclic β-peptide was still impossible, we observed the peptide nanotubes successfully by AFM. We tried to find mite condition to obtain a single peptide nanotube from these observations.

在允许单个螺旋自由旋转的状态下将螺旋连接到支架上。将肽铺展在水上，并将形成的单层转移到金上。不能获得肽在金上的STM图像，可能是因为肽没有牢固地固定在表面上。然后，将肽修饰为在每个螺旋的N末端具有二硫基。将修饰的肽掺入到十二硫醇盐的自组装单层中。利用扫描隧道显微镜对修饰肽进行了观察。值得注意的是，修饰的肽响应于肽上施加的偏置电压而在金上移动。然而，肽高度保持恒定。通过三个点将肽连接到金应该是固定恒定高度的原因。另一方面，利用环状β-肽构建肽纳米管。尽管环β-肽的单分子图像仍然是不可能的，我们成功地观察到肽纳米管的AFM。我们试图找到螨条件，以获得一个单一的肽纳米管从这些观察。

项目成果

Double Assembly Composed of Lectin Association with Columnar Molecular Assembly of Cyclic Tri-b-peptide Having Sugar Units

凝集素与含糖单元环三b肽柱状分子组装体组成的双重组装体

• 发表时间: 2007
• 期刊: Biomacromolecules 3
• 作者: F. Fujimura;Y. Horikawa;J. Sugiyama;T. Morita;S. Kimura
• 通讯作者: S. Kimura

Vertical and Directional Insertion of Helical Peptide into Lipid Bilaver Memberane

螺旋肽垂直定向插入脂质双膜

• 发表时间: 2007
• 期刊: Langmuir 23
• 作者: K.;Nakatani;T.;Morita;S.;Kimura
• 通讯作者: Kimura