Study on Mechanism of a Direct Photosynthetic/Metabolic Bio-Fuel Cell (DPBFC) using Gene manipulated Bacteria and Prototyping of Flexible DPBFC

利用基因操纵细菌研究直接光合/代谢生物燃料电池（DPBFC）的机理以及柔性 DPBFC 的原型设计

• 批准号: 18360122
• 负责人: MORISHIMA Keisuke
• 金额: $ 7.64万
• 依托单位: Tokyo University of Agriculture and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18360122/
• 关键词: Fuel cell; DNA; Bacteria; Micromachine; Bioreactor

In our laboratory, we have developed a mediator-less direct photosynthetic/metabolic bio-fuel cell (DPBFC) in which microparticles of polyaniline were adopted as the electrode material to extract electrons from bacteria In this paper, we selected Rhodopseudomonas palustris as a new fuel source which is activated by organic compounds and emits hydrogen. To improve the electron generation efficiency of R.palustris, gene manipulation was applied so as to suppress hydrogen emission and more electrons will be stored inside of R.palustris. As a result, the power density obtained by the mutant was 18.3μW/cm^2, which is higher than the wild type. In addition, to extend the cell life, it seems necessary to supply a medium to bacteria continuously, therefore, we have developed a medium flow system using water-absorbing polymer. The average flow rate was 32nl/min and the cell life for the mutant became 12 hours, that is six times longer than the previous DPBFC. In addition, we have prototyped a thin type flexible DPBFC by using a heat-sealing film and thermocompression process. As a result, the developed thin type flexible DPBFC(50×50×1mm) could generate the maximum power 64μW(4.65μW/cm2) when purple photosynthetic bacteria (Rhodopesudomonas palustris) was used as a fuel source.

本实验室开发了一种无介质的直接光合/代谢生物燃料电池（DPBFC），该电池采用聚苯胺微粒作为电极材料从细菌中提取电子。本文选择了红假单胞菌（Rhodopseudomonas palustris）作为一种新的燃料源，该燃料源被有机化合物激活并释放氢。为了提高稻瘟神的电子生成效率，通过基因操作抑制了稻瘟神的氢发射，使稻瘟神体内储存了更多的电子。结果表明，突变体的功率密度为18.3μW/cm^2，高于野生型。此外，为了延长细胞寿命，似乎有必要持续为细菌提供介质，因此，我们开发了一种采用吸水聚合物的介质流系统。平均流速为32nl/min，突变体的细胞寿命为12小时，比之前的DPBFC延长了6倍。此外，我们还利用热封膜和热压工艺制作了一种薄型柔性DPBFC原型。结果表明，当紫色光合细菌（Rhodopesudomonas palustris）作为燃料源时，所研制的薄型柔性DPBFC（50×50×1mm）可产生64μW（4.65μW/cm2）的最大功率。

项目成果

Improving the performance of a direct photosynthetic/metabolic bio-fuel cell(DPBFC)using gene manipulated bacteria

使用基因操纵细菌提高直接光合/代谢生物燃料电池（DPBFC）的性能

• 发表时间: 2007
• 期刊: Journal of Micromechanics And Microengineering, Institute of Physics Publishing Vol.17, No.9
• 作者: Keisuke Morishima;Makoto Yoshida;Akihiro Furuya;Takeyuki Moriuchi;Masahiro Ota and Yuji Furukawa
• 通讯作者: Masahiro Ota and Yuji Furukawa

光合成細菌を用いたバイオフューエルセルのフレキシブル構造化

利用光合细菌的生物燃料电池的柔性结构

• 发表时间: 2008
• 作者: 森内 健行;角田 史郎;森島 圭祐;古川 勇二
• 通讯作者: 古川 勇二

Genetic Conversion of Bacteria and its Application to Micro Direct Photosynthetic/Metabolic Bio-Fuel Cell(DPMFC)

细菌的遗传转化及其在微型直接光合/代谢生物燃料电池（DPMFC）中的应用

• 发表时间: 2006
• 期刊: Proceedings of the 10^th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS), Tokyo, Japan, November,(2006) Volume 1
• 作者: Akihiro Furuya;Takeyuki Moriuchi;Makoto Yoshida;Masahiro Ota;Keisuke Morishima;Yuji Furukawa
• 通讯作者: Yuji Furukawa

Design prisnciple and prototyping of a direct photosynthetic/metabolic biofuel cell(DPMFC)

直接光合/代谢生物燃料电池（DPMFC）的设计原理和原型制作

• 发表时间: 2006
• 期刊: Journal Of Micromechanics And Microengineering, Institute Of Physics Publishing, Vol.16, No.9
• 作者: Yuji Furukawa;Takeyuki Moriuchi and Keisuke Morishima
• 通讯作者: Takeyuki Moriuchi and Keisuke Morishima

Improvement of power capability applying pyrolyzed carbon photoresist electrode for Micro Direct Photosynthetic/metabolic Bio-Fuel Cell(DPBFC)

应用热解碳光刻胶电极提高微型直接光合/代谢生物燃料电池（DPBFC）的功率能力

• 发表时间: 2007
• 作者: Takeyuki Moriuchi;Keisuke Morishima and Yuji Furukawa
• 通讯作者: Keisuke Morishima and Yuji Furukawa