Development of petide array for diagnosis fo important protozoan diseases using one drop of blood sample.

开发肽阵列，使用一滴血样诊断重要的原生动物疾病。

• 批准号: 18380181
• 负责人: IGARASHI Ikuo
• 金额: $ 11.92万
• 依托单位: Obihiro University of Agriculture and Veterinary Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18380181/
• 关键词: 重要原虫病; 網羅的; 診断; ペプチド(プロテイン)アレイ; 血清; 少量試料; 血清少量試料; プロテインアレイ; 少量; ペプチドアレイ

Protozoan diseases such as babesiosis cause great economical losses to animal industry world-wide. Many amimals are imported from foreign countries to Japan every year, and it is important to prevent invasion of infected these animals to Japan at the edge of the water. Objective of the present study was to develop the serodiagnostic test that was able to diagnose protozoan diseases with one drop of the blood sample. The protein array using recombinant antigens were successfully established to detect two bovine Babesia species at the same time. By adding other protozoan antigens, novel serological diagnostics will be established for diagnosis of many protozoan diseases at a time in the future.

原生动物疾病（例如Babesiosis）在全球范围内给动物行业造成了巨大的经济损失。每年都会从国外进口许多款项，重要的是要防止在水边缘侵入这些动物到日本。本研究的目的是开发血清诊断测试，该测试能够用一滴血液样本诊断原生动物疾病。成功建立了使用重组抗原的蛋白质阵列，以同时检测两种牛Babesia物种。通过添加其他原生动物抗原，将来将建立新的血清学诊断来诊断许多原生动物疾病。

项目成果

Development of loop-mediated isothermal amplification (LAMP) method for diagnosis of equine piroplasmosis

• DOI: 10.1016/j.vetpar.2006.08.014
• 发表时间: 2007-01-31
• 期刊: VETERINARY PARASITOLOGY
• 影响因子: 2.6
• 作者: Alhassan, Andy;Thekisoe, Oriel M. M.;Igarashi, Ikuo
• 通讯作者: Igarashi, Ikuo

Inhibitory effect of (-)Epigallocatechin3 gallate from green tea on the growth of Babesia parasites

绿茶中的(-)表没食子儿茶素3没食子酸酯对巴贝虫寄生虫生长的抑制作用

• 发表时间: 2009
• 作者: Abou-Laila M.;横山直明;五十嵐郁男
• 通讯作者: 五十嵐郁男

Growth inhibitory effectof terpene nerolidol against Babesia parasites

萜烯橙花叔醇对巴贝斯虫的生长抑制作用

• 发表时间: 2008
• 作者: Abou-Laila M;T. Sivakumar;N. Yokoyama;I. Igarashi
• 通讯作者: I. Igarashi

ウシバベシア2種を同時に検出可能なプロテインアレイの開発

开发可同时检测两种牛巴贝虫的蛋白质阵列

• 发表时间: 2010
• 作者: 玉城志緒;井関博;周麗佳;小山亜紀;横山直明;J.Sataporn;五十嵐郁男
• 通讯作者: 五十嵐郁男

Diagnostic real-time PCR assay for the quantitative detection of Theileria equi from equine

用于定量检测马泰勒虫的诊断性实时 PCR 测定

• 发表时间: 2008
• 期刊: Vet. Parasitol. 151
• 作者: Kim CM;Blanco LB;Alhassan A;Ise
• 通讯作者: Ise