Epidemiology on piroplasmosis of demestic animals in Mongolia

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Babesiosis in domestic animals causes enormous economic losses throughout most of the world. In Mongolia, high prevalence of babesiosis was observed in horses but not known in cattle. In the present study, in vitro cultivation was used for isolation of Babesia parasites from Mongolian horses and geneticanalysis of isolated parasites has done. Detection of antibody and DMA fragments of bovine Babesia parasites were also analyzed.1. Antigen analysis of equine Babesia parasites and survey of antibodiesRecombinant proteins of B. equi (MEA-1) and B. caballi (BC48) were found to be useful as antigens for serodiagnostic methods such as ELISA or latex agglutination test. Both B. equi and B. caballi are endemic in horses in Central Mongolia as 81. 8% of horses were seropositive to both infections in 110 samples by IFAT. A total of 209 horse sera obtained from 2 different areas of Central Mongolia were examined by ELISA using GST-BC48. The prevalence of B. caballi infection in the 2 different ar … More eas was 90.3 % and 23.4 %.2. Isolation of equine Babesia parasites by in vitro cultivation.B. equi parasites were found in 33 samples from 40 blood samples. Analysis of DNA sequence of EMA-1 and EMA-2 suggests that B. equi has high genetic homology among isolates and that B. equi isolated in Mongolia has high homology in nucleotide and amino acid sequences with USDA strain.3. Survey on ticks transmittable Babesia parasitesEMA-1 gene of B. equi and BC48 gene of B. caballi were detected in all samples and seven of the 54 samples of Dermacenor nuttalli adult ticks by PCR, respectively.4. Survey on bovine babesiosisRecombinant rhoptry associated protein-1 (RAP-1) of Babesia bovis was produced and i used as antigen in ELISA. The ELISA was able to differentiate between B. bovis-infected sera and B. bigemina-infected sera or noninfected normal bovine sera. The results demonstrate that the recombinant RAP-1 expressed in high five insect cells might be a useful antigen for detection of antibodies to B. bovis. Less