Epidemiology on piroplasmosis of demestic animals in Mongolia

蒙古国家畜焦虫病流行病学

• 批准号: 11691167
• 负责人: IGARASHI Ikuo
• 金额: $ 4.22万
• 依托单位: Obihiro University of Agriculture and Veterinary Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11691167/
• 关键词: piroplasmosis; Mongolia; horse; cattle; antibody survey; isolation of parasites; diagnostic antigens; genetic analysis; バベシア原虫; 組み換え抗原; ELISA; PCR; 抗体

Babesiosis in domestic animals causes enormous economic losses throughout most of the world. In Mongolia, high prevalence of babesiosis was observed in horses but not known in cattle. In the present study, in vitro cultivation was used for isolation of Babesia parasites from Mongolian horses and geneticanalysis of isolated parasites has done. Detection of antibody and DMA fragments of bovine Babesia parasites were also analyzed.1. Antigen analysis of equine Babesia parasites and survey of antibodiesRecombinant proteins of B. equi (MEA-1) and B. caballi (BC48) were found to be useful as antigens for serodiagnostic methods such as ELISA or latex agglutination test. Both B. equi and B. caballi are endemic in horses in Central Mongolia as 81. 8% of horses were seropositive to both infections in 110 samples by IFAT. A total of 209 horse sera obtained from 2 different areas of Central Mongolia were examined by ELISA using GST-BC48. The prevalence of B. caballi infection in the 2 different ar … More eas was 90.3 % and 23.4 %.2. Isolation of equine Babesia parasites by in vitro cultivation.B. equi parasites were found in 33 samples from 40 blood samples. Analysis of DNA sequence of EMA-1 and EMA-2 suggests that B. equi has high genetic homology among isolates and that B. equi isolated in Mongolia has high homology in nucleotide and amino acid sequences with USDA strain.3. Survey on ticks transmittable Babesia parasitesEMA-1 gene of B. equi and BC48 gene of B. caballi were detected in all samples and seven of the 54 samples of Dermacenor nuttalli adult ticks by PCR, respectively.4. Survey on bovine babesiosisRecombinant rhoptry associated protein-1 (RAP-1) of Babesia bovis was produced and i used as antigen in ELISA. The ELISA was able to differentiate between B. bovis-infected sera and B. bigemina-infected sera or noninfected normal bovine sera. The results demonstrate that the recombinant RAP-1 expressed in high five insect cells might be a useful antigen for detection of antibodies to B. bovis. Less

家畜中的巴贝病在世界大部分地区造成巨大的经济损失。在蒙古，在马匹中观察到了辣椒症的高患病率，但在牛中不知道。在演讲研究中，使用体外培养来分离蒙古马匹的贝贝斯寄生虫，而分离的寄生虫的遗传分析已经完成。还分析了牛巴贝斯寄生虫的抗体和DMA片段的检测。1。马贝西亚寄生虫的抗原分析和对Equi（MEA-1）和B. caballi（BC48）的抗体重组蛋白的调查被发现可作为抗原的血清诊断方法，例如ELISA或乳胶凝集测试。在蒙古中部的马匹中，Equi和B. b. caballi都是内在的，为81。8％的马在110个样本中通过IFAT在110个样本中的两种感染都是血清的。 ELISA使用GST-BC48检查了从蒙古中部2个不同地区获得的209匹马血清。在2种不同的AR中，Caballi感染的患病率是90.3％和23.4％.2。通过体外栽培分离马贝氏寄生虫。在40个血液样本的33个样品中发现了寄生虫。对EMA-1和EMA-2的DNA序列的分析表明，B. Equi在分离株之间具有很高的遗传同源性，而B.在蒙古中分离的B.等效分离在具有USDA菌株的核苷酸和氨基酸序列中具有很高的同源性。3。在所有样品中，PCR分别检测到所有样品和54个Dermacenor nuttalli成人tick虫中的tick虫的调查。对牛babeia babesia bobesia babesia相关蛋白1（RAP-1）进行了牛Babesiosis Recispinans的调查，并生产了我在ELISA中用作抗原。 ELISA能够区分B. bovis感染的血清和B. bigemina感染的血清或未感染的正常牛血清。结果表明，在高五个昆虫细胞中表达的重组RAP-1可能是检测抗牛芽孢杆菌抗体的有用抗原。较少的

项目成果

Hirata, H., Ikadai, H., Yokoyama, N., Xpan, X., Fujisaki , K., Suzuki, N., Mikami, T. and Igarashi, I.: "Cloning of a truncated Babesia equi gene encoding a 82-kilodalton protein and its potential use in an enzyme-linked immunosorbent assay"J. Clin. Micro

Hirata, H.、Ikadai, H.、Yokoyama, N.、Xpan, X.、Fujisaki, K.、Suzuki, N.、Mikami, T. 和 Igarashi, I.：“克隆编码 a 的截短马巴贝虫基因

Ikadal H.,Osorio C.R.,Xuan X.,Igarashi I.et al.: "Detection of Babesia caballi infection by enzyme-linked immunosorbent assay using recombinant 48-kDa merozoite rhoptry protein"International Journal for Parasitology. 27(in press). (2000)

Ikadal H.、Osorio C.R.、Xuan X.、Igarashi I.等人：“使用重组 48-kDa 裂殖子菱形蛋白通过酶联免疫吸附测定检测巴贝虫感染”国际寄生虫学杂志。

Xuan,X.,Larsen,A.,Ikadai,H.,Tanaka,T.,Igarashi,I. et al.: "Expression of Babesia equi merozoite antigen-1 in insect cells by recombinant baculovirus and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay."J.Clin.Micorbiol.. 39.

旋，X.，拉森，A.，Ikadai，H.，田中，T.，五十岚，I。

Tanaka T.,Xuan X.,Ikadai H.,Igarashi I.et al.: "Expression of Babesia equi merozoite antigen-2 by recombinant baculovirus and its use in the ELISA"International Journal for Parasitology. 26. 1803-1808 (1999)

Tanaka T.、Xuan X.、Ikadai H.、Igarashi I.等人：“重组杆状病毒表达马贝贝虫裂殖子抗原 2 及其在 ELISA 中的应用”国际寄生虫学杂志。

Xuan, X., Larsen, A., Ikadai, H., Tanaka, T., Igarashi, I. Et al.: "Expression of Babesia equi merozoite antigen-1 in insect cells by recombinant baculovirus and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay"J. Clin. Micor

Xu, X., Larsen, A., Ikadai, H., Tanaka, T., Igarashi, I. 等人：“通过重组杆状病毒在昆虫细胞中表达马贝斯虫裂殖子抗原 1 并评估其诊断潜力