A basic study on the development of bioartificial pancreas using a highly differentiated human pancreatic beta cell line.

使用高度分化的人胰腺β细胞系开发生物人工胰腺的基础研究。

• 批准号: 18390347
• 负责人: KOBAYASHI Naoya
• 金额: $ 11.5万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18390347/
• 关键词: Diabetes; Cell Tissue; Tissue Eneineerin; Regenerative Medicine; Biotechnology

We originally established a highly differentiated human pancreatic beta cell line. Therefore, we performed a basic study on the development of an implantable typed bioartificial pancreas (BAP) using the cell line for the treatment of diabetes.1. The human pancreatic beta cell line was functionally cultured with a self-assembling peptide, PuraMatrix, to form islet-like struchture.2. We constructed a BAP device in which the inner side is composed of high-density polyethylene (HDPE) coated with cellular adhesive polypeptides and the outer side is the membrane of ethylene venyl alcohol (EVAL). Freshly isolated rat islets attached on the surface of HDPE very well.3. The BAP device was implanted in the intraabdominal cavity with be lapped by the greater omentum. When the outer side of the BAP device was coated by gelatinized bFGF, the number of the induced vessels around the device increased at 1.2-fold.4. When 2,000 of freshly isolated rat islets injected into the BAP device, function of the islets were maintained for 28 days in vitro.

我们最初建立了一个高度分化的人类胰腺β细胞系。因此，我们对利用该细胞系开发用于治疗糖尿病的植入式生物人工胰腺（BAP）进行了基础研究。用一种自组装肽（PuraMatrix）对人胰岛β细胞系进行功能培养，形成胰岛样结构。我们构建了一种BAP装置，其内层由高密度聚乙烯（HDPE）包被细胞粘附多肽，外层为乙烯乙烯醇（EVAL）膜。新鲜分离的大鼠胰岛在HDPE表面附着良好。BAP装置植入腹腔内，经大网膜包裹。当BAP装置外侧涂有糊化bFGF时，装置周围的诱导血管数量增加了1.2倍。将2000个新鲜分离的大鼠胰岛注射到BAP装置中，体外维持胰岛功能28天。

项目成果

新鮮分離イヌ膵島の機能的な培養法に関する基礎的研究.

犬新鲜分离胰岛功能培养方法的基础研究

• 发表时间: 2007
• 作者: Rivas-Carrillo;Jorge David;三木 厚;小林 直哉;窪田 康浩
• 通讯作者: 窪田 康浩

An extra-cellular matrix-coated intelligent fabric maintains and improves islet function

细胞外基质涂层的智能织物可维持和改善胰岛功能

• 发表时间: 2006
• 作者: Alejandro;Soto-Gutierrez
• 通讯作者: Soto-Gutierrez

埋め込み式バッグ型バイオ人工膵臓を用いた糖尿病犬治療.

使用植入式袋式生物人工胰腺治疗糖尿病犬。

• 发表时间: 2007
• 作者: Rivas-Carrillo;Jorge David;三木 厚;小林 直哉
• 通讯作者: 小林 直哉

ベイオ人工膵臓開発に向けた基礎的検討.

生物人工胰腺开发的基础研究。

• 发表时间: 2006
• 作者: Alejandro;Soto-Gutierrez;Jorge David Rivas-Carrillo;小林 直哉
• 通讯作者: 小林 直哉

Pancreas development and beta-cell differentiation of embryonic stem cells.

胰腺发育和胚胎干细胞的β细胞分化。

• 发表时间: 2007
• 期刊: Current medicinal chemistry 14
• 作者: Kishi Y;Sugawara Y;Tamura S;Kaneko J;Matsui Y;Makuuchi M;小林直哉;Jorge David Rivas-Carrillo
• 通讯作者: Jorge David Rivas-Carrillo