A basic study on the development of bioartificial pancreas using a highly differentiated human pancreatic beta cell line.

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We originally established a highly differentiated human pancreatic beta cell line. Therefore, we performed a basic study on the development of an implantable typed bioartificial pancreas (BAP) using the cell line for the treatment of diabetes.1. The human pancreatic beta cell line was functionally cultured with a self-assembling peptide, PuraMatrix, to form islet-like struchture.2. We constructed a BAP device in which the inner side is composed of high-density polyethylene (HDPE) coated with cellular adhesive polypeptides and the outer side is the membrane of ethylene venyl alcohol (EVAL). Freshly isolated rat islets attached on the surface of HDPE very well.3. The BAP device was implanted in the intraabdominal cavity with be lapped by the greater omentum. When the outer side of the BAP device was coated by gelatinized bFGF, the number of the induced vessels around the device increased at 1.2-fold.4. When 2,000 of freshly isolated rat islets injected into the BAP device, function of the islets were maintained for 28 days in vitro.