Adequate toothbrushing method following periodontal tissue regeneration

牙周组织再生后适当的刷牙方法

• 批准号: 18390564
• 负责人: MORITA Manabu
• 金额: $ 5.3万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18390564/
• 关键词: periodontal tissue regenerative therapy; initiation of tooth-brushing; experimental periodontitis; Newly-formed alveolar bone

The purpose of the present study was to determine an adequate stage for initiating tooth-brushing and optimal tooth-brushing force/duration following periodontal tissue regenerative therapy.Six beagle dogs were involved in the experiment First, the maxillary second premolar and mandibular third premolar were extracted. 4x4x4 mm of size, was formed between the maxillary first and third premolars and between the mandibular second and fourth premolars. Then, notch was marked on the root surface for recording the bottom of bone defect. In order to induce periodontitis, metal matrix was inserted in the area of bone defect The dogs were then kept for 8 weeks with being fed by soft food. After these treatments, the metal matrix were removed and periodontal tissue regenerative therapy was performed using Emdogain. Experimental teeth were divided into four groups according to the split-mouth design for each dog. The control tooth was given daily plaque removal using a hand instrument. The other three was given daily professional tooth-brushing following 1 day (1D), 3 days (3D), or 7 days (7D) after the periodontal regenerative therapy. The force and duration of tooth-brushing was set for 200g and 20 seconds, respectively. The stress gauze was used for controlling the tooth-brushing force.Histological observation found similar results with respect to gingival inflammation and remodeling of alveolar bone. No marked inflammation and down growth of sulcular epithelium was observed in all the groups. Newly-formed alveolar bone was found around the one-third of initial bone defect area. A little amount of newly formed cementum was observed around the notch. However, epithelial downgrowth was partially observed for 1D, 3D dogs.The results of this study indicate that tooth-brushing can be initiated next day after periodontal surgical treatment.

本研究的目的是确定牙周组织再生治疗后开始刷牙的适当阶段和最佳刷牙力/持续时间。实验涉及六只比格犬。首先，拔除上颌第二前磨牙和下颌第三前磨牙。大小为 4x4x4 毫米，形成于上颌第一和第三前磨牙之间以及下颌第二和第四前磨牙之间。然后，在牙根表面标记凹口以记录骨缺损的底部。为了诱发牙周炎，将金属基质植入骨缺损区域。然后将狗饲养8周，喂软食。经过这些治疗后，去除金属基质并使用 Emdogain 进行牙周组织再生治疗。根据每只狗的分口设计，实验牙齿被分为四组。对照牙齿每天使用手持器械去除牙菌斑。另外三人在牙周再生治疗后 1 天 (1D)、3 天 (3D) 或 7 天 (7D) 后每天进行专业刷牙。刷牙的力度和时间分别设定为200克和20秒。使用应力纱布来控制刷牙力。组织学观察发现牙龈炎症和牙槽骨重塑有类似的结果。所有组均未观察到明显的炎症和龈沟上皮的向下生长。在初始骨缺损区域的三分之一周围发现了新形成的牙槽骨。在切口周围观察到少量新形成的牙骨质。然而，在 1D、3D 狗中观察到部分上皮向下生长。这项研究的结果表明，牙周手术治疗后第二天就可以开始刷牙。