Study of intra-species diversity in Giardia intestinalig Assessment of a genotype distribution in isolated islands of Indonesia

肠贾第鞭毛虫种内多样性研究 印度尼西亚孤岛基因型分布评估

• 批准号: 18406007
• 负责人: TOKORO Masaharu
• 金额: $ 3.73万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18406007/
• 关键词: parasite; intestinal protozoon; Indonesia; intra-species diversity; molecular epidemiology; genotype; phvlogenv; zoonotic transmission; ジアルジア; 種内変異; インドネシア:パレスチナ; 地理的分布

We aimed to determine the status of intra-species genetic diversity of Giardia intestinalis and the factors that contribute to the maintenance of population diversity in this species. Giardia samples collected from various mammals (dog, pig, mouse and human) i n different geographical sites(Indonesia, Nepal and Japan) were analyzed using multilocus markers (18S small subunit ribosomal RNA, glutamate dehydrogenase and triosephosphate isomerase genes). In the human samples, various sub-genotypes within assemblage A and/or B were clearly classified ; however, no site-specific distribution of genotypes was observed. We also confirmed that assemblages C and D, which have been recognized as dog-specific genotypes, were present in a few human samples in a rural area of Indonesia. A comparison of the determined sequences of human and animal isolates collected at the same site specifically confirmed the presence of each sub-assemblage cluster within assemblage B.Taken together with previous indications of assemblage-level host specificities in G. intestinalis, these results demonstrate that host specificities could be partly responsible for the assemblage levels of genetic polymorphism in this pathogen, however the diversity within each assemblage especially assemblage B seemed not in the case. Although the present geographical distributions and isolations of the pathogen were not thought to be a driving force of its genetic diversity since the distribution did not indicate any locality, we showed here the possibility that niche separations in a site might be related to the diversity formation. Niche separation may enable Giardia to accumulate the unique mutations in the genome and maintain novel diversity within the clonal population.

本研究的目的是探讨贾第鞭毛虫种内遗传多样性的现状以及维持该种种群多样性的因素。采用多位点标记（18 S小亚基核糖体RNA、谷氨酸脱氢酶和磷酸丙糖异构酶基因）分析了从不同地理位置（印度尼西亚、尼泊尔和日本）的各种哺乳动物（狗、猪、小鼠和人）采集的贾第虫样品。在人类样本中，集合A和/或B内的各种亚基因型被明确分类;然而，没有观察到基因型的位点特异性分布。我们还证实，组合C和D，这已被确认为狗的特定基因型，存在于一些人的样本在印度尼西亚的农村地区。在同一地点收集的人和动物分离物的测定序列的比较明确地证实了组合B内每个亚组合簇的存在。这些结果表明，寄主特异性可能是造成该病原菌遗传多态性聚集水平的部分原因，而各聚集体尤其是聚集体B内的多样性则不一定是这种情况。虽然目前的地理分布和隔离的病原体被认为是其遗传多样性的驱动力，因为分布并不表明任何地方，我们在这里显示的可能性，在一个网站的生态位分离可能与多样性的形成。生态位分离可能使贾第虫积累基因组中的独特突变，并保持克隆种群内的新多样性。

项目成果

Genotyping of Cyclospora cayetansis: An assessment of zoonosis potential

卡耶坦环孢子虫的基因分型：人畜共患病潜力的评估

• 发表时间: 2007
• 作者: Tajima K;et. al.;Tomoyo Yoshida
• 通讯作者: Tomoyo Yoshida

Analysis of Cryptosporidium alternative oxidase

隐孢子虫替代氧化酶的分析

• 发表时间: 2006
• 作者: Harada;T
• 通讯作者: T

Parasitic Zoonoses in Asian-Pacific Regions 2006

2006年亚太地区寄生虫病

• 发表时间: 2006
• 作者: Hussein AIA;Nakamoto K;Yamaguchi T;Yoshida T;Tokoro M;Masaharu Tokoro
• 通讯作者: Masaharu Tokoro

クリプトスポリジウム症の治療又は予防薬

隐孢子虫病的治疗或预防

• 发表时间: 2008

Subgenotype analysis of Cryptosporidium parvum isolates from humans and animals in Japan using the 60-kDa glycoprotein gene sequences

• DOI: 10.1007/s00436-006-0140-0
• 发表时间: 2006-08-01
• 期刊: PARASITOLOGY RESEARCH
• 影响因子: 2
• 作者: Abe, Niichiro;Matsubayashi, Makoto;Iseki, Motohiro
• 通讯作者: Iseki, Motohiro