The effects of anesthesia on internal gene expression

采用实时荧光定量RT-PCR方法，观察吸入麻醉药（七氟醚、异氟醚）和静脉麻醉药（丙泊酚、右美托咪定）对大鼠脑、肺、肝组织基因表达的影响。吸入麻醉剂在麻醉期间抑制脑中的昼夜节律基因（Per 2、Dbp、Arc、Ergl、Krox 2、NGFI-B）表达，并且该作用在唤醒后持续24小时。静脉麻醉剂显示类似的效果，但不影响DBP的基因表达。在肺组织中，麻醉药在麻醉过程中增强了调节肺循环的ET-1、NOS 3和ADM基因的表达。在肝脏中，大多数麻醉药在麻醉过程中增强某些药物代谢酶（Cyp 7a 1，Cyp 2b 15，Por，Nrli 2，Ces 2，Ugtla 7，Abcb 1a，Abcc 2）的基因表达，并且在唤醒后24小时这种作用消失。本研究结果支持了我们以前用微阵列技术检测内源性基因表达的研究，表明：1）全身麻醉对麻醉过程中内源性基因表达有影响，这种影响可能在苏醒后持续存在;（2）不同麻醉方法和不同麻醉药对基因表达的影响不同，影响程度也不同。

Using realtime RT-PCR method, the effects of inhalation anesthetics (sevoflurane, isoflurane) and intravenous anesthetics (propofol, dexmedetomidine) on intrenal gene expression were measured in rats brain, lung, and liver. Inhalation anesthetics suppressed circadian genes (Per2, Dbp, Arc, Ergl, Krox2, NGFI-B) expression in the brain during anesthesia and the effects persisted 24 hrs after awakening. Intravenous anesthetics showed similar effects, but did not influence gene expression of Dbp. In the lung, anesthetics reinforced genes expression of ET-1, NOS3, and ADM, which regulated pulmonary circulation, during anesthesia. In the liver, most anesthetics reinforced gene expression of some drug metabolizing enzymes (Cyp7a1, Cyp2b15, Por, Nrli2, Ces2, Ugtla7, Abcb1a, Abcc2) during anesthesia and the effects disappeared 24 hrs after awakening. The pattern of the influences were partly different between inahaltion and intravenous anesthetics.The results of this study supported our previous study that examined the internal gene expression using microarray method, and showed that 1) general anesthesia affects the internal gene expression during anesthesia and the effects may persist after awakening, 2) the genes to be influenced by and the degree to influence gene expression are different between different anesthesia methods and between different anesthetics.