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The effects of anesthesia on internal gene expression

麻醉对内部基因表达的影响

基本信息

批准号：
18591728
负责人：
SAKAMOTO Atsuhiro
金额：
$ 2.45万
依托单位：
Nippon Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591728/
关键词：
anesthetics anesthesia gene expression circadian gene 麻酔日内変動脳肺

项目摘要

Using realtime RT-PCR method, the effects of inhalation anesthetics (sevoflurane, isoflurane) and intravenous anesthetics (propofol, dexmedetomidine) on intrenal gene expression were measured in rats brain, lung, and liver. Inhalation anesthetics suppressed circadian genes (Per2, Dbp, Arc, Ergl, Krox2, NGFI-B) expression in the brain during anesthesia and the effects persisted 24 hrs after awakening. Intravenous anesthetics showed similar effects, but did not influence gene expression of Dbp. In the lung, anesthetics reinforced genes expression of ET-1, NOS3, and ADM, which regulated pulmonary circulation, during anesthesia. In the liver, most anesthetics reinforced gene expression of some drug metabolizing enzymes (Cyp7a1, Cyp2b15, Por, Nrli2, Ces2, Ugtla7, Abcb1a, Abcc2) during anesthesia and the effects disappeared 24 hrs after awakening. The pattern of the influences were partly different between inahaltion and intravenous anesthetics.The results of this study supported our previous study that examined the internal gene expression using microarray method, and showed that 1) general anesthesia affects the internal gene expression during anesthesia and the effects may persist after awakening, 2) the genes to be influenced by and the degree to influence gene expression are different between different anesthesia methods and between different anesthetics.

采用实时荧光定量RT-PCR方法，观察吸入麻醉药（七氟醚、异氟醚）和静脉麻醉药（丙泊酚、右美托咪定）对大鼠脑、肺、肝组织基因表达的影响。吸入麻醉剂在麻醉期间抑制脑中的昼夜节律基因（Per 2、Dbp、Arc、Ergl、Krox 2、NGFI-B）表达，并且该作用在唤醒后持续24小时。静脉麻醉剂显示类似的效果，但不影响DBP的基因表达。在肺组织中，麻醉药在麻醉过程中增强了调节肺循环的ET-1、NOS 3和ADM基因的表达。在肝脏中，大多数麻醉药在麻醉过程中增强某些药物代谢酶（Cyp 7a 1，Cyp 2b 15，Por，Nrli 2，Ces 2，Ugtla 7，Abcb 1a，Abcc 2）的基因表达，并且在唤醒后24小时这种作用消失。吸入麻醉剂和静脉麻醉剂的影响模式有部分不同。本研究结果支持了我们之前使用微阵列方法检测内部基因表达的研究，并表明1）全身麻醉影响麻醉期间内部基因表达，并且这种影响可能会持续醒来后，（2）不同麻醉方法和不同麻醉药对基因表达的影响不同，影响程度也不同。