Integrated examination of the effects of general anesthetics on genomics, proteomics and metabolomics

全身麻醉对基因组学、蛋白质组学和代谢组学影响的综合检查

• 批准号: 20591820
• 负责人: SAKAMOTO Atsuhiro
• 金额: $ 3万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591820/
• 关键词: 麻酔薬; 遺伝子発現; 蛋白変動; 代謝物変動; 日内変動遺伝子

The effects of general anesthetics on the changes of internal gene expression, protein expression and metabolites were evaluated by genomics, proteomics, and metabolomics. The main results are as follows. (1) The general anesthetics affects the clock gene expression in the brain and influences circadian rhythm. (2) The inhalational anesthetic influences the change of protein expression in the brain more than intravenous anesthetic. (3) The intravenous anesthetic influences the brain metabolism more than inhalational anesthetic. These results suggested the need of the further molecular biologic examination according to the each biological system.

用基因组学、蛋白质组学和代谢组学的方法评价全麻药对脑内基因表达、蛋白质表达和代谢产物变化的影响。主要研究结果如下。(1)全麻药影响脑内时钟基因的表达，影响昼夜节律。(2)吸入麻醉药对脑内蛋白质表达变化的影响大于静脉麻醉药。(3)静脉麻醉药对脑代谢的影响大于吸入麻醉药。这些结果提示有必要根据不同的生物系统进行进一步的分子生物学检测。

项目成果

The general anesthetic sevoflurane effects the expression of clock gene mPer2 accompanying the change of NAD(+) level in the suprachiasmatic nucleus of mice.

全身麻醉药七氟烷影响小鼠视交叉上核时钟基因mPer2的表达并伴随NAD()水平的变化。

• 发表时间: 2011
• 期刊: Neuroscience Letter
• 作者: Ohe Y;Sakamoto A;et al.
• 通讯作者: et al.

The reciprocal relationship between heme oxygenase and nitric oxide syanthase in the organs of lipopolysaccharide-treated rodents.

脂多糖处理的啮齿动物器官中血红素加氧酶和一氧化氮合酶之间的相互关系。

• 发表时间: 2009
• 期刊: Biomedical Research 30
• 作者: Furuichi M.;Sakamoto A.;et al.
• 通讯作者: et al.

Expressions of genes encoding drug-metabolizing enzymes are altered after sevoflurane, isoflurane, propofol or dexmedetomidine anesthesia

• DOI: 10.2220/biomedres.30.17
• 发表时间: 2009-02-01
• 期刊: BIOMEDICAL RESEARCH-TOKYO
• 影响因子: 1.2
• 作者: Nakazato, Keiko;Yoshida, Yuko;Sakamoto, Atsuhiro
• 通讯作者: Sakamoto, Atsuhiro

Pattern recognition analysis of proton nuclear magnetic resonance spectra of brain tissue extracts from rats anesthetized with propofol or isoflurane.

• DOI: 10.1371/journal.pone.0011172
• 发表时间: 2010-06-17
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Kawaguchi H;Hirakawa K;Miyauchi K;Koike K;Ohno Y;Sakamoto A
• 通讯作者: Sakamoto A

セボフルラン、イソフルラン麻酔後のラット肝臓における薬物代謝系遺伝子発現への影響

七氟醚和异氟醚对异氟醚麻醉后大鼠肝脏药物代谢基因表达的影响

• 发表时间: 2008
• 作者: 中里桂子;坂本篤裕;他
• 通讯作者: 他