CELL-BIOLOGICAL AND GRAPHIC STUDIES OF GROWTH DISTURBANCE AND SCLEROTIC CHANGE OF MASTOID AIR CELLS

乳突气囊生长障碍和硬化变化的细胞生物学和图形研究

• 批准号: 18591873
• 负责人: TAKAHASHI Haruo
• 金额: $ 2.44万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591873/
• 关键词: apoptosis; mastoid; osteoblast; osteoclast; otitis media; TUNEL staining; pneumatization

To examine the presence or absence of apoptotic participation in the process of normal development of the mastoid pneumatization in human, we performed immunohistochemical examinations of the tissue cells in the mastoid pneumatized bones harvested from 26 patients without history or signs of otitis media, who underwent mastoid surgery in our department clinic. We used the TdT-mediated dUTP nick end-labeling (TUNEL)method for observing apoptosis cells, which revealed TUNEL positive cells in 13 of 26 cases, and these cells were found in 84.6% of children under the age of 5,being significantly greater number than in those older than 6 years of age. Furthermore, in a sample from a child with otitis media, TUNEL positive cell was not observed. These results can be interpreted as having a good correlation between the presence or absence of TUNEL positive cells and growth of mastoid pneumatization, and indicated that apoptosis might contribute to the normal growth of mastoid pneumatization. By identifying those TUNEL positive cells and by examining cytokines related to those cells, the cell biological mechanisms of growth of pneumatization and thus the mechanism and protective measures of its inhibition.

为探讨细胞凋亡是否参与人乳突气化骨的正常发育过程，我们对26例无中耳炎病史或体征的患者进行了乳突气化骨组织细胞的免疫组织化学检测。采用TdT介导的dUTP缺口末端标记法(TUNEL)观察凋亡细胞，发现26例中有13例TUNEL阳性，其中5岁以下儿童占84.6%，明显高于6岁以上儿童。此外，在一个患有中耳炎的儿童的样本中，没有观察到TUNEL阳性细胞。以上结果提示，TUNEL阳性细胞的存在与乳突气化灶的生长密切相关，提示细胞凋亡可能参与了乳突气化灶的正常生长。通过对TUNEL阳性细胞的鉴定和与这些细胞相关的细胞因子的检测，探讨气雾化生长的细胞生物学机制，进而探讨气化气抑制的机制和保护措施。

项目成果

Otitis Media with Effusion

• DOI: 10.1891/9780826195197.0135
• 发表时间: 2019-01
• 期刊: Canadian Family Practice Guidelines
• 作者: J. Cash;Kathleen Bradbury-Golas

Apoptosis in the process of development of human mastoid pneumatization

人乳突气化发育过程中的细胞凋亡

• 作者: Fujiyama;D.;Fukuda;T.;Takahashi;H.;et. al.
• 通讯作者: et. al.

ヒト乳突蜂巣の発育におけるアポトーシスの関与の検討-予報

检查细胞凋亡在人类乳突蜂窝发育中的作用 - 预测

• 发表时间: 2008
• 期刊: 長崎医学会雑誌 83(印刷中)
• 作者: 藤山 大祐;高橋 晴雄;他
• 通讯作者: 他

ヒト乳突蜂巣の正常発育におけるアポトーシスの関与の検討

细胞凋亡参与人乳突蜂窝正常发育的研究

• 发表时间: 2007
• 作者: 藤山 大祐、高橋 晴雄;他
• 通讯作者: 他

Otitis media and pneumatization

中耳炎和气化

• 发表时间: 2008
• 期刊: J Otolaryngol Head Neck Surg(JOHNS) 24
• 作者: Fujiyama;D.;Fukuda;T.;Takahashi;H
• 通讯作者: H