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Visualization of retinal ganglion cells ex vivo using full-field optical coherence tomography

使用全场光学相干断层扫描对视网膜神经节细胞进行离体可视化

基本信息

批准号：
18591917
负责人：
HANGAI Masanori
金额：
$ 2.57万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591917/
关键词：
optical coherence tomography glaucoma retinal ganglion cell retinal nerve fiber layer

项目摘要

Full-held optical coherence tomography (FF-OCT) using Halogen lamp was evaluated a vivo in pig eye cups. Effects of varying band widths of light source, fields of view and numbers of CCD pixel on imaging performance of retina were compared_ We fund a best setting composing 120 nm band width, 850 μm x850 μm, and 500 x 500 pixels of CCD for imaging retinal cellular structures from clinical point of view. The 120 nm band width gives a 2μm of axial resolution. In this setting, retinal ganglion cells were depicted as round hyporeflective spots. In addition, nerve fiber bindles were depicted in the retinal nerve fiber layer. Next, we tried to reconstruct 3-dimensional images by using volume rendering technique on serial OCT sections. The retinal ganglion cells were depicted hyporeflective balls. In addition, fiber-like structures were visualized in the retinal nerve fiber layer. Retinal ganglion cells are distributed in the 3-dimensional space. Therefore, it is impossible to count the number of retinal ganglion cells by using single sections. The 3-dimensional visualization of retinal ganglion cells allow for counting their numbers. The current OCT technology allows measurement of thickness of retinal nerve fiber layer. However, measurement of retinal nerve fiber layer thickness has the risk that the space in which retinal nerve fiber bundles are lost are also measured. Visualization of nerve fiber bundles by FF-OCT allows measurement of only the actual amount of nerve fiber bundles. Thus, FF-OCT at our setting opens a new avenue to study glaucomatous damage on retinal ganglion cells and nerve fiber bundles. If this technology works in live human eyes, it would make an epoch-making progress in the diagnosis of glaucoma. Our results give evidence supporting the motivation that clinical application of FF-OCT technology.

使用卤素灯的全持式光学相干断层扫描(FF-OCT)在猪眼杯上进行了活体评估。比较了不同光源带宽、不同视场和不同像素数对视网膜成像性能的影响。从临床角度出发，确定了由120 nm带宽、850μm×850μm、500×500像素的CCD500×500像素组成的视网膜细胞结构成像的最佳设置。120 nm的带宽提供了2μm的轴向分辨率。在此背景下，视网膜神经节细胞被描绘为圆形低反射点。此外，视网膜神经纤维层内可见神经纤维束。接下来，我们尝试在连续的OCT切片上使用体绘制技术重建三维图像。视网膜神经节细胞被描绘为低反射球。此外，视网膜神经纤维层内可见纤维样结构。视网膜神经节细胞分布于三维空间。因此，不可能用单个切片来计算视网膜神经节细胞的数量。视网膜神经节细胞的三维可视化允许对它们的数量进行计数。目前的OCT技术可以测量视网膜神经纤维层的厚度。然而，测量视网膜神经纤维层厚度有风险，即视网膜神经纤维束丢失的空间也会被测量。FF-OCT对神经纤维束的可视化仅允许测量神经纤维束的实际数量。因此，在我们的环境下，FF-OCT为研究青光眼对视网膜神经节细胞和神经纤维束的损害开辟了一条新的途径。如果这项技术在活体人眼中起作用，它将在青光眼诊断方面取得划时代的进步。我们的结果支持了FF-OCT技术临床应用的动机。