权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

DOES HEAT SHOCK PROTEIN 27 INDUCE DIFFERENTIATION OF TISSUE STEM CELLS TO PROGENITOR CET IS?

热休克蛋白 27 是否会诱导组织干细胞向祖细胞 CET 分化？

基本信息

批准号：
18592006
负责人：
AMANO OSAMU
金额：
$ 2.37万
依托单位：
Meikai University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18592006/
关键词：
heat shock protein Hsp25 27 submandibular gland duct ligation acinar regeneration proliferation differentiation tissue stem cell rat Hsp27 口蓋発生唾液腺再生歯肉付着上皮アポトーシス

项目摘要

Heat shock protein 27 (Hsp27) has been suggested to participate in the cell proliferation and differentiation during tissue development. In fact, we have demonstrated the transient occurrence of Hsp27 during the differentiation of salivary gland acinar cells in postnatal rats. The purpose of the present study is to explore the potential role of Hsp27 in the proliferation and differentiation of the acinar cells during regeneration of the salivary gland. Using the experimental regeneration model of the rat submandibular gland after the release of duct ligation, the spatio-temporal localization of Hsp27 was investigated in immunohistochemistry in regenerating acini.No epithelial cells were immunoreactive for Hsp27 immediately after unligation, but Hsp27-immunoreactive cells were observed in regenerating acini located at the end portion of survived ductal tissues on the third day after unligation. The number of Hsp27-immunoreactive cells in regenerating acini reached its peak on the 5th day after unligation, and started to decline on the 7th day. They were undetectable on the 14th day. Importantly, the increase in the number of Hsp27-immunoreactive cells was preceded by the decline in the cell proliferative activity, and Hsp27-immunoreactivity declined and disappeared in conjunction with the progression of acinar cell differentiation, as judged by the double-immunostaining for Hsp27 and proliferating cell nuclear antigen, a cell proliferation marker, or glycine-rich protein-a, a specific marker of differentiated acinar cells.All the findings suggest that Hsp27 is expressed with the transition from the cell proliferation to differentiation of the acinar precursor cells during the regenerating process.

热休克蛋白27（Heat shock protein 27，Hsp 27）在组织发育过程中参与细胞增殖和分化。事实上，我们已经证明了在出生后大鼠的唾液腺腺泡细胞的分化过程中，热休克蛋白27的瞬时发生。本研究的目的是探讨热休克蛋白27在涎腺再生过程中腺泡细胞增殖和分化中的作用。应用大鼠颌下腺导管结扎后再生模型，用免疫组织化学方法研究了Hsp 27在再生腺泡中的时空定位，结果发现：结扎后即刻，再生腺泡上皮细胞中未见Hsp 27免疫反应阳性细胞，而结扎后第3天，在存活导管组织的末端部分再生腺泡中可见Hsp 27免疫反应阳性细胞。再生腺泡内Hsp 27免疫反应阳性细胞数在未结扎后第5天达到高峰，第7天开始下降。在第14天，它们是不可检测的。重要的是，Hsp 27免疫反应性细胞数量的增加之前是细胞增殖活性的下降，并且Hsp 27免疫反应性随着腺泡细胞分化的进展而下降和消失，如通过Hsp 27和增殖细胞核抗原（细胞增殖标记物）或富含甘氨酸的蛋白-a的双重免疫染色所判断的，提示Hsp 27在腺泡前体细胞增殖分化过程中有表达。