Role of novel transcription factor on bone formation

新型转录因子对骨形成的作用

• 批准号: 18592032
• 负责人: KAWAI Shinji
• 金额: $ 2.44万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18592032/
• 关键词: Osteoblast; Differentiation; Tramsgenic mice; Transcription factor; Bone formation; Microarray

We report that Osr2 is one of the regulators of osteoblast function, since dominant-negative Osr2 transgenic mice exhibited decreased osteoblast activity, as well as delayed mineralization in calvarial and tibial bone tissues. Our results indicate that Osr2 functions in regulation of osteoblast proliferation. Molecular mechanisms that control bone formation have received attention with increasing knowledge related to genetic control of osteoblast differentiation. The odd-skipped related (Osr) gene is a zinc-finger transcription factor recently suggested to be involved in bone formation, though little is known about its role. To elucidate the in vivo function of Osr2, we generated transgenic mice overexpressing dominant-negative Osr2. In the present study, N-terminal deleted Osr2 was found to act as a dominant-negative mutant toward both Osr1 and Osr2. Dominant-negative Osr2 (Osr2ΔN) transgenic mice demonstrated delayed mineralization in calvarial and cortical bone tissues. Further, soft X-ray analysis of transgenic mice bones revealed distinctly increased radiolucency. Examinations of newborn Osr2ΔN transgenic mice skeletons stained with alcian blue and alizarin red showed reduced intensities in the skull and skeletal elements. Morphologically, calvaria and tibia of Osr2ΔN transgenic mice were composed of markedly thinner parietal and cortical bones, and lower numbers of osteoblastic cells on bone surfaces, indicating a reduced proliferation of osteoblasts. Further, calvarial osteoblasts obtained from Osr2ΔN transgenic mice showed highly attenuated osteoblast differentiation and proliferation, confirming that Osr2 is required for osteogenesis. Finally, results of Runx2-deficient cell assays suggested that Osr2 induces ALP expression, but to a lesser degree than Runx2-expressing cells. Our genetic observations showed that the Osr2 gene plays a key role in osteoblastic cell proliferation.

我们报告说，Osr 2是成骨细胞功能的调节器之一，因为显性负Osr 2转基因小鼠表现出成骨细胞活性下降，以及在颅骨和胫骨组织矿化延迟。我们的研究结果表明，Osr 2的功能在成骨细胞增殖的调节。随着对成骨细胞分化的遗传控制的认识不断增加，控制骨形成的分子机制受到关注。奇数跳跃相关基因（Osr）是一种锌指转录因子，最近被认为与骨形成有关，但对其作用知之甚少。为了阐明Osr 2的体内功能，我们产生了过表达显性负性Osr 2的转基因小鼠。在本研究中，N-末端缺失Osr 2被发现作为一个显性负突变体对Osr 1和Osr 2。显性阴性Osr 2（Osr 2 ΔN）转基因小鼠表现出颅骨和皮质骨组织矿化延迟。此外，转基因小鼠骨骼的软X射线分析显示射线可透性明显增加。用阿辛蓝和茜素红染色的新生Osr 2 ΔN转基因小鼠骨骼的检查显示头骨和骨骼元素的强度降低。在形态学上，Osr 2 ΔN转基因小鼠的颅骨和胫骨由明显变薄的顶骨和皮质骨组成，骨表面成骨细胞数量减少，表明成骨细胞增殖减少。此外，从Osr 2 ΔN转基因小鼠获得的颅骨成骨细胞显示出高度减弱的成骨细胞分化和增殖，证实Osr 2是骨生成所必需的。最后，Runx 2缺陷细胞测定的结果表明，Osr 2诱导ALP表达，但程度低于Runx 2表达细胞。我们的遗传学观察表明，Osr 2基因在成骨细胞增殖中起着关键作用。

项目成果

Distribution of Porphyromonas gingivalis fimA genotypes in cardiovascular specimens from Japanese patients

• DOI: 10.1111/j.1399-302x.2007.00406.x
• 发表时间: 2008-04-01
• 期刊: ORAL MICROBIOLOGY AND IMMUNOLOGY
• 作者: Nakano, K.;Inaba, H.;Ooshima, T.
• 通讯作者: Ooshima, T.

Molecular interaction of alanine-rich and proline-rich regions of cell surface prothin antigen PAc in Streptococcus mutans.

变形链球菌细胞表面蛋白抗原 PAc 富含丙氨酸和富含脯氨酸区域的分子相互作用。

• 发表时间: 2008
• 期刊: Oral Microbilolgy and Immunology (in press)
• 作者: Matsumoto-Nakano M;et. al.
• 通讯作者: et. al.

Disruption of epithelial barrier and impairment of cellular function by Porphyromonas gingivalis

• DOI: 10.2741/2363
• 发表时间: 2007-05-01
• 期刊: FRONTIERS IN BIOSCIENCE
• 作者: Amano, Atsuo

Serotype distribution of Streptococcus mutans, a pathogen of dental caries, in cardiovascular specimens from Japanese patients

日本患者心血管标本中龋齿病原体变形链球菌的血清型分布

• 发表时间: 2007
• 期刊: J Med Microbiol 56
• 作者: Nakano K;Nemoto H;Nomura R;Homma H;Yoshioka H;Shudo Y;Hata H;Toda K;Taniguchi K;Amano A;Ooshima T
• 通讯作者: Ooshima T

Virulence of porphyromonas gingivalis is altered by substitution of fimbria gene wity different genotype

不同基因型菌毛基因的替换改变了牙龈卟啉单胞菌的毒力

• 发表时间: 2006
• 期刊: Cell Microbiology 9(3)
• 作者: Kato T;et. al.
• 通讯作者: et. al.