Molecular dissection of transcription factor on bone formation

转录因子对骨形成的分子解析

• 批准号: 21592356
• 负责人: KAWAI Shinji
• 金额: $ 2.83万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592356/
• 关键词: 口腔生化学; 骨芽細胞; 細胞分化; トランスジェニックマウス; 転写因子; 骨形成; マイクロアレイ

To elucidate the in vivo function of Osr1, we generated transgenic mice overexpressing Osr1. Osr1 transgenic mice demonstrated delayed cranial sutures. Examinations of newborn Osr1 transgenic mice skeletons stained with alcian blue and alizarin red showed abnormalities in the skull and skeletal elements. Calvarial osteoblasts obtained from Osr1 transgenic mice showed abnormalities in osteoblast differentiation and proliferation, confirming that Osr1 is required for osteogenesis. Our genetic observations showed that the Osr1 gene plays a key role in osteoblastic cell proliferation.

为了阐明Osr1的体内功能，我们产生了过表达Osr1的转基因小鼠。Osr1转基因小鼠表现出延迟颅缝。用阿辛蓝和茜素红染色的新生Osr1转基因小鼠骨骼检查显示头骨和骨骼元素异常。从Osr1转基因小鼠获得的颅骨成骨细胞显示成骨细胞分化和增殖异常，证实Osr1是骨生成所必需的。我们的遗传学观察表明，Osr1基因在成骨细胞增殖中起着关键作用。

项目成果

Odd-skipped related 2 regulates genes related to proliferation and development.

• DOI: 10.1016/j.bbrc.2010.06.054
• 发表时间: 2010-07
• 期刊: Biochemical and biophysical research communications
• 影响因子: 3.1
• 作者: S. Kawai;Y. Abiko;A. Amano

Odd-skipped relatedl and Odd-skipped related2 are mutually regulated

奇数跳过相关1和奇数跳过相关2相互调节

• 发表时间: 2011
• 作者: Fujita K;Nozaki Y;Wada K;Yoneda M;Fujimoto Y;et al.;Shinji Kawai
• 通讯作者: Shinji Kawai

Cellular ATP Synthesis Mediated by Type III Sodium-dependent Phosphate Transporter Pit-1 Is Critical to Chondrogenesis

• DOI: 10.1074/jbc.m110.148403
• 发表时间: 2011-01-28
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Sugita, Atsushi;Kawai, Shinji;Yoneda, Toshiyuki
• 通讯作者: Yoneda, Toshiyuki

Odd-skipped related1とOdd-skipped related2の相互的な発現制御

奇数跳过相关1和奇数跳过相关2的相互表达调节

• 发表时间: 2011
• 作者: 河合伸治;天野敦雄
• 通讯作者: 天野敦雄

Expression of Odd-Skipped Related 2 in Dental Mesenchyme and Renal Corpuscle

奇数跳跃相关2在牙间充质和肾小体中的表达

• 发表时间: 2009
• 作者: Kawai S;Amano A
• 通讯作者: Amano A